RH38_ORYSJ
ID RH38_ORYSJ Reviewed; 505 AA.
AC Q10RI7; A0A0P0VTA7; Q8H8C3;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=DEAD-box ATP-dependent RNA helicase 38;
DE EC=3.6.4.13;
GN OrderedLocusNames=Os03g0158200, LOC_Os03g06220; ORFNames=OJ1134F05.6;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: ATP-dependent RNA helicase essential for mRNA export from the
CC nucleus. Plays an important role in the positive regulation of CBF/DREB
CC transcription factors (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus {ECO:0000250}.
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX19/DBP5
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN06835.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF10934.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC099401; AAN06835.1; ALT_SEQ; Genomic_DNA.
DR EMBL; DP000009; ABF94071.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF10934.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014959; BAS82383.1; -; Genomic_DNA.
DR RefSeq; XP_015631989.1; XM_015776503.1.
DR AlphaFoldDB; Q10RI7; -.
DR SMR; Q10RI7; -.
DR STRING; 4530.OS03T0158200-01; -.
DR PaxDb; Q10RI7; -.
DR PRIDE; Q10RI7; -.
DR EnsemblPlants; Os03t0158200-01; Os03t0158200-01; Os03g0158200.
DR GeneID; 4331680; -.
DR Gramene; Os03t0158200-01; Os03t0158200-01; Os03g0158200.
DR KEGG; osa:4331680; -.
DR eggNOG; KOG0332; Eukaryota.
DR HOGENOM; CLU_003041_1_0_1; -.
DR InParanoid; Q10RI7; -.
DR OMA; DFKNLCM; -.
DR OrthoDB; 608788at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q10RI7; OS.
DR GO; GO:0010494; C:cytoplasmic stress granule; IBA:GO_Central.
DR GO; GO:0005635; C:nuclear envelope; IEA:EnsemblPlants.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003729; F:mRNA binding; IEA:EnsemblPlants.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR GO; GO:0016973; P:poly(A)+ mRNA export from nucleus; IBA:GO_Central.
DR GO; GO:0009737; P:response to abscisic acid; IEA:EnsemblPlants.
DR GO; GO:0009409; P:response to cold; IEA:EnsemblPlants.
DR GO; GO:0009408; P:response to heat; IEA:EnsemblPlants.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; Helicase; Hydrolase; mRNA transport;
KW Nucleotide-binding; Nucleus; Reference proteome; RNA-binding; Transport.
FT CHAIN 1..505
FT /note="DEAD-box ATP-dependent RNA helicase 38"
FT /id="PRO_0000282472"
FT DOMAIN 134..310
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 338..493
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 100..129
FT /note="Q motif"
FT MOTIF 254..257
FT /note="DEAD box"
FT COMPBIAS 10..28
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 147..154
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 505 AA; 55855 MW; 7EDD95B2C3180D28 CRC64;
MADGGKPPTP EKKSWADVEE EEEAKAKAAA AAEAASSSSS NEPAVDAQAK QIEALSLSVP
EEHGGSGGGG DDQGPPLLDD SDESQIQAVT SGGTVYESAA AFEDLKLTPE LLKGLHDEMG
FSRPSKIQAV TLPMILTPPY KDLIAQAHNG SGKTTCFVLG MLSRVDPNRK VTQAICICPT
RELAQQNKSV LMRMGKFTGI TCACAIPPAQ KDYVPIAKMP KITDQVVIGT SGTLMKWINH
KKILTNDIKI LVFDEADHML AEDGFRSDSE RIMRDIQRSA GGCQVLLFSA TFNERVKDFV
TRVIKDGNQI FVKKEELTLE KVKQYKVQVP DERAKIAVIK DKIFEFGQKV GQVIIFVRTK
QSTKDVHNAL TLEDYVCSSI QGSLDQSERE KIIQEFKNGY TKVLISTDVL ARGFDQAQVN
LVINYDMPIK FGTRDEPDYE VYLHRIGRAG RFGRKGAVFN LLCGETDNTV MRKIETYFQH
NVPEVRNWQS EEDFERALKD AGLVE
