RH45_ORYSJ
ID RH45_ORYSJ Reviewed; 947 AA.
AC Q0J7Y8;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 2.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=DEAD-box ATP-dependent RNA helicase 45;
DE EC=3.6.4.13;
GN OrderedLocusNames=Os08g0154200, LOC_Os08g05810;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX46/PRP5
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF22927.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP008214; BAF22927.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014964; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; Q0J7Y8; -.
DR SMR; Q0J7Y8; -.
DR STRING; 4530.OS08T0154225-00; -.
DR PaxDb; Q0J7Y8; -.
DR PRIDE; Q0J7Y8; -.
DR eggNOG; KOG0334; Eukaryota.
DR InParanoid; Q0J7Y8; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 3.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding; Coiled coil; Helicase; Hydrolase; Nucleotide-binding;
KW Reference proteome; RNA-binding.
FT CHAIN 1..947
FT /note="DEAD-box ATP-dependent RNA helicase 45"
FT /id="PRO_0000282502"
FT DOMAIN 316..494
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 479..647
FT /note="Helicase C-terminal"
FT REGION 1..132
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 159..221
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 658..710
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 854..879
FT /evidence="ECO:0000255"
FT MOTIF 285..313
FT /note="Q motif"
FT MOTIF 442..445
FT /note="DEAD box"
FT COMPBIAS 11..61
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 174..197
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 198..215
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 672..710
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 329..336
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 947 AA; 104832 MW; 3FAED1BAE01692F5 CRC64;
MEEEEVVVVV DEEESERRRQ KMIEEEKKRL DEEMELRRRR VKEWQEQKRL EEEEAKRREQ
EAAAGAGTPA AAAGADGDSN AGKKWTLDGE ESDEEGYKED SQNAEDDGGI TADLPSEVND
ANVAAPMEED EIDPLDAFMS SMVLPEVAKL ETAVASMESM PASNMGDKNG KSAKDAVSNG
DKKGQKKAMG RIMQGDDSDS DYDDDDDDEG GSKDEDDEEF MKRVKKTKVE KLAIVDHSKI
EYQPFRKNLY IEVKDITMMT GEEVATYRKN LELKVHGKDV PKPIKTWVQS GLTSKLLDTI
KKLGFEKPMP IQAQALPIIM SGRDCIGIAK TGSGKTLAFV LPMLRHVKDQ PPVVPGDGPI
GLIMAPTREL VVQIHSDIKK FAKSLGINCV AIYGGSGVAQ QISELKRGAE IVVCTPGRMI
DILCTSSGKI TNLRRVTFLV MDEADRMFDM GFEPQITRIV QNTRPDRQTV LFSAIFPRQV
EILARKVLTK PVEIQVGGRS VVNKDITQLV EVRPENERFL RLLELLGEWF DRGKILVFVH
SQDKCDSLLK DLFQRGYPCL SLHGGKDQTD RESTLADFKS NLELVVNYDV PNHYEDYVHR
VGRTGHAGRK GFAVTFISDE EERYAPDLAK ALELSEQAVP QDLKGLADRF MAKVKQGTEQ
AHGTGYGGSG FKFNEEEDEA RRSAKKAQAR EYGYEEDKSD SDSDEEGGVR KAGGDLAAQA
IAAAQAAATL AAAKAASNAN QQVQSTNAGS LLSIPVVANA PNNEATARAL QAALNIQQNL
ARIQAHVVPE HYEVELDIND FPQNARWKIT HKETLGPIQD WTEAAITTRG TFIPQGKIVG
ANERKLYLFI EGPTELSVKK AKSELKRVLE DCANHALNLP GSAQTGKTAN SEMQGFLVKV
FLGRWTAILV FLDDGVICNI RAEKLDKWQV RVFYVKVADP LGYSITE
