RH48_ARATH
ID RH48_ARATH Reviewed; 798 AA.
AC Q9C8S9;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Probable DEAD-box ATP-dependent RNA helicase 48;
DE EC=3.6.4.13;
GN Name=RH48; OrderedLocusNames=At1g63250; ORFNames=F9N12.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17168887; DOI=10.1111/j.1467-7652.2004.00084.x;
RA Mingam A., Toffano-Nioche C., Brunaud V., Boudet N., Kreis M., Lecharny A.;
RT "DEAD-box RNA helicases in Arabidopsis thaliana: establishing a link
RT between quantitative expression, gene structure and evolution of a family
RT of genes.";
RL Plant Biotechnol. J. 2:401-415(2004).
RN [4]
RP GENE FAMILY.
RX PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA Xu R., Zhang S., Huang J., Zheng C.;
RT "Genome-wide comparative in silico analysis of the RNA helicase gene family
RT in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza
RT sativa.";
RL PLoS ONE 8:E78982-E78982(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. {ECO:0000305}.
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DR EMBL; AC022355; AAG52143.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34076.1; -; Genomic_DNA.
DR PIR; C96658; C96658.
DR RefSeq; NP_176514.1; NM_105004.3.
DR AlphaFoldDB; Q9C8S9; -.
DR SMR; Q9C8S9; -.
DR IntAct; Q9C8S9; 1.
DR STRING; 3702.AT1G63250.1; -.
DR iPTMnet; Q9C8S9; -.
DR PaxDb; Q9C8S9; -.
DR PRIDE; Q9C8S9; -.
DR ProteomicsDB; 236168; -.
DR EnsemblPlants; AT1G63250.1; AT1G63250.1; AT1G63250.
DR GeneID; 842631; -.
DR Gramene; AT1G63250.1; AT1G63250.1; AT1G63250.
DR KEGG; ath:AT1G63250; -.
DR Araport; AT1G63250; -.
DR TAIR; locus:2038431; AT1G63250.
DR eggNOG; KOG0342; Eukaryota.
DR HOGENOM; CLU_003041_26_6_1; -.
DR InParanoid; Q9C8S9; -.
DR OMA; MMPLIAR; -.
DR OrthoDB; 537587at2759; -.
DR PhylomeDB; Q9C8S9; -.
DR PRO; PR:Q9C8S9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C8S9; baseline and differential.
DR Genevisible; Q9C8S9; AT.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW RNA-binding.
FT CHAIN 1..798
FT /note="Probable DEAD-box ATP-dependent RNA helicase 48"
FT /id="PRO_0000239188"
FT DOMAIN 359..543
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 577..726
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 76..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 117..148
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 236..257
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 328..356
FT /note="Q motif"
FT MOTIF 491..494
FT /note="DEAD box"
FT COMPBIAS 129..148
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 372..379
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 798 AA; 89591 MW; 2DD59E4A9BFB9710 CRC64;
MYSLILRERS GSITGSLWNR ISSRNMGGGP RTFPGGLNKW QWKRMHEKKA REKENKLLDQ
EKQLYEARIR TEIRAKMWGN PDSGEKTAKS KQSHGPMSPK EHIKTLADRF MKAGAEDFWN
ENDGPVKKSD QGSRSGSDSI DSTSNSPIDV RRLVSATCDS MGKNRVFGSS RRGFSSMSRF
KRNESSCDEG DDFDAKKLDT LSPFSPKFAG TKEKVKSSRS VVGVIRNKGL FGRRKFRKND
SSTEEDSDEE GDEGKMIGWM DMRKTGSSAS LGNHDIKLTK RVNRNVTDEE LYPPLDINTV
REDLSKRKSV DNVMEEKQEP HDSIYSAKRF DESCISPLTL KALSASGILK MTRVQDATLS
ECLDGKDALV KAKTGTGKSM AFLLPAIETV LKAMNSGKGV NKVAPIFALI LCPTRELASQ
IAAEGKALLK FHDGIGVQTL IGGTRFKLDQ QRLESEPCQI LIATPGRLLD HIENKSGLTS
RLMALKLFIV DEADLLLDLG FRRDVEKIID CLPRQRQSLL FSATIPKEVR RVSQLVLKRD
HSYIDTIGLG CVETHDKVRQ SCIVAPHESH FHLVPHLLKE HINNTPDYKI IVFCSTGMVT
SLMYTLLREM KLNVREIHAR KPQLHRTRVS DEFKESNRLI LVTSDVSARG MNYPDVTLVI
QVGIPSDREQ YIHRLGRTGR EGKGGEGLLL IAPWERYFLD ELKDLPLEPI PAPDLDSIVK
HQVDQSMAKI DTSIKEAAYH AWLGYYNSVR ETGRDKTTLA ELANRFCHSI GLEKPPALFR
RTAVKMGLKG ISGIPIRK
