ID   RH50_ORYSJ              Reviewed;         641 AA.
AC   Q0DVX2; A0A0P0VS39; B7EBC5; Q10SX0; Q8H7P8;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2007, sequence version 2.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=DEAD-box ATP-dependent RNA helicase 50;
DE            EC=3.6.4.13;
GN   OrderedLocusNames=Os03g0108600, LOC_Os03g01830;
GN   ORFNames=OJ1384D03.13, OsJ_09113;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [7]
RP   FUNCTION, AND INDUCTION BY BIOTIC AND ABIOTIC DEFENSE STIMULI.
RX   PubMed=18441339; DOI=10.1093/jxb/ern072;
RA   Li D., Liu H., Zhang H., Wang X., Song F.;
RT   "OsBIRH1, a DEAD-box RNA helicase with functions in modulating defence
RT   responses against pathogen infection and oxidative stress.";
RL   J. Exp. Bot. 59:2133-2146(2008).
CC   -!- FUNCTION: Probably involved in resistance to biotic and abiotic
CC       stresses. Confers tolerance to oxidative stress and mediates
CC       pathogenesis-related (PR) genes expression. Exhibits RNA-dependent
CC       ATPase and ATP-dependent RNA helicase activities in vitro.
CC       {ECO:0000269|PubMed:18441339}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- INDUCTION: In leaves after treatment with defense-related signal
CC       chemicals such as benzothiadiazole (BTH), salicylic acid (SA), l-
CC       aminocyclopropane-1-carboxylic acid (ethylene-precursor), and jasmonic
CC       acid (JA). Also up-regulated during incompatible interaction with the
CC       blast fungus Magnaporthe grisea. {ECO:0000269|PubMed:18441339}.
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC       family of RNA helicases and controls ATP binding and hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN62787.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAF10616.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AC113930; AAN62787.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; DP000009; ABF93566.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAF10616.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AP014959; BAS81898.1; -; Genomic_DNA.
DR   EMBL; CM000140; EEE58182.1; -; Genomic_DNA.
DR   EMBL; AK065776; BAG89672.1; -; mRNA.
DR   AlphaFoldDB; Q0DVX2; -.
DR   SMR; Q0DVX2; -.
DR   STRING; 4530.OS03T0108600-01; -.
DR   PaxDb; Q0DVX2; -.
DR   PRIDE; Q0DVX2; -.
DR   EnsemblPlants; Os03t0108600-01; Os03t0108600-01; Os03g0108600.
DR   Gramene; Os03t0108600-01; Os03t0108600-01; Os03g0108600.
DR   eggNOG; KOG0331; Eukaryota.
DR   HOGENOM; CLU_003041_24_1_1; -.
DR   InParanoid; Q0DVX2; -.
DR   OMA; AYFERDR; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000007752; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; Q0DVX2; OS.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IDA:UniProtKB.
DR   GO; GO:0006968; P:cellular defense response; IDA:UniProtKB.
DR   GO; GO:0071369; P:cellular response to ethylene stimulus; IEP:UniProtKB.
DR   GO; GO:0071395; P:cellular response to jasmonic acid stimulus; IEP:UniProtKB.
DR   GO; GO:0071446; P:cellular response to salicylic acid stimulus; IEP:UniProtKB.
DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Plant defense;
KW   Reference proteome; RNA-binding.
FT   CHAIN           1..641
FT                   /note="DEAD-box ATP-dependent RNA helicase 50"
FT                   /id="PRO_0000282519"
FT   DOMAIN          271..452
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          487..641
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   REGION          86..115
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          129..189
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          197..216
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           240..268
FT                   /note="Q motif"
FT   MOTIF           399..402
FT                   /note="DEAD box"
FT   COMPBIAS        149..184
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         284..291
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   641 AA;  70803 MW;  466E8B0956FF2028 CRC64;
     MEVAGAQAGI LPLLLRHPAS LRGSLSLSCG GARRSWAAAA ATAEGGGGEE GRGYERVPMD
     TPGAYRLVDR ATGRSVIVWG GTDDVSMPSP AVLSTTTRVP DRPKENGRST SIGNFGRLKA
     QKVKVLARRS AHLKREDSGR ISTSRFSESP SDESDEDGTY FERDRARNTR QNSRSRDDKT
     RGAHSLNSVL RQYRGADDLD FPGSEATSGS KRWGNISDVT FGRQNQRQKG PLDSGFFSRR
     SFKEIGCSDE ILGALRSFGF PRPSHIQAMA YRPVLEGKSC IIGDQSGSGK TLAYLCPVVQ
     NLRKEEVEGL HRSSPRNPRV VVLTPTAELA SQVLNNCRSI SKSGVPFRSM VATGGFRQKT
     QLESLDQELD VLIATPGRFL YLLQEGFVQL NNLRCVVLDE VDILYGEESF EQVLHQLITV
     APLTTQYLFV TATLPLDIYN KVVETFPDCE LIMGPGVHRT SSRLEEILVD CSGDDNEEKN
     PETAFSNKKS ALVKIIEESP VRKTIIFCNK IETCRKVENA LRRVDRKASQ IKVLPFHAAL
     DQQQRIANIK EFLNKQTADS MFLVCTDRAS RGIDFANVNH VVLFDYPRDP SEYVRRVGRT
     ARGASGNGKA FVFAVGKQVS LARRVMERNI KGHPLHDVPC V