RH52A_ORYSJ
ID RH52A_ORYSJ Reviewed; 602 AA.
AC Q0DB53; Q69XK1;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 2.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=DEAD-box ATP-dependent RNA helicase 52A;
DE EC=3.6.4.13;
GN OrderedLocusNames=Os06g0602400, LOC_Os06g40020; ORFNames=P0486H12.18;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 119-602.
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX3/DED1
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF19920.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AP003615; BAD35456.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF19920.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014962; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK106474; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015641985.1; XM_015786499.1.
DR AlphaFoldDB; Q0DB53; -.
DR SMR; Q0DB53; -.
DR STRING; 4530.OS06T0602400-01; -.
DR PaxDb; Q0DB53; -.
DR PRIDE; Q0DB53; -.
DR GeneID; 4341457; -.
DR KEGG; osa:4341457; -.
DR eggNOG; KOG0335; Eukaryota.
DR HOGENOM; CLU_003041_16_3_1; -.
DR InParanoid; Q0DB53; -.
DR OrthoDB; 595675at2759; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR Genevisible; Q0DB53; OS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR CDD; cd17967; DEADc_DDX3_DDX4; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR044763; Ded1/Dbp1_DEADc.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW RNA-binding.
FT CHAIN 1..602
FT /note="DEAD-box ATP-dependent RNA helicase 52A"
FT /id="PRO_0000282450"
FT DOMAIN 115..305
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 328..485
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 9..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 492..521
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 552..602
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 84..112
FT /note="Q motif"
FT MOTIF 249..252
FT /note="DEAD box"
FT BINDING 128..135
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 602 AA; 63008 MW; 864D55BFAFD4D3B9 CRC64;
MAAAAAVAKS VEAGGEPGGG GGGAWSTVSR SGRSSYSAGG GVGGGKVGEL AEGLAGVEIG
GERRLDKYDI PVEVSGEDVP PPADGFEAAG LVEAVLRNVA RCGYESPTPV QRYSMPIALA
GRDLMACAQT GSGKTAAFCL PVVSGLVAAG GSGIGHRERS SFNRAAAKPR ALVLAPTREL
AAQINEEAKK FSFQTGLRVV VAYGGTPMYN QLRDLERGAD ILVATPGRLV DMVERSKVSL
EAIKYLVMDE ADRMLDMGFE PQIRKIVERM NMPRKSVRQT MLFSATFPPE IQRLASDFLS
NYIFITVGRV GSSTDLIMQK VELLSDGEKR GYLLDLLQRQ SVGVANSKLQ QPLTLVFVET
KREADSLRYW LYSKGFPATA IHGDRTQQER ESALRSFKTG LTPIMVATDV ASRGLDVPNV
AHVINYDLPK SIEDYVHRIG RTGRAGKAGS ATAFFTESDH SLAKGLLELM TEAKQDVPDW
LVQYAERPYY GGSSYGGRNR RSGGGGNRFA GRDFRQGSGG GYSGGGGGGG YSGGGGGGGY
SGGGGGYSGG GRGGGYSRGG RGGYSGGGGG GGGDPYRASA PPPRYYPSYP MGTADINASG
WD
