RH52B_ORYSJ
ID RH52B_ORYSJ Reviewed; 638 AA.
AC Q6Z4K6; B9FW25; Q9MAW8;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=DEAD-box ATP-dependent RNA helicase 52B;
DE EC=3.6.4.13;
DE AltName: Full=OsPL10b;
GN Name=PL10B; OrderedLocusNames=Os07g0202100, LOC_Os07g10250;
GN ORFNames=OsJ_23477 {ECO:0000312|EMBL:EEE66762.1}, OSJNBa0081K20.14;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 217-330.
RA Mochizuki K.;
RT "PL10-like genes in rice.";
RL Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX3/DED1
CC subfamily. {ECO:0000305}.
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DR EMBL; AP005171; BAC83834.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF21049.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT00508.1; -; Genomic_DNA.
DR EMBL; CM000144; EEE66762.1; -; Genomic_DNA.
DR EMBL; AK101736; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AB042644; BAA95705.1; -; mRNA.
DR RefSeq; XP_015646994.1; XM_015791508.1.
DR AlphaFoldDB; Q6Z4K6; -.
DR SMR; Q6Z4K6; -.
DR STRING; 4530.OS07T0202100-01; -.
DR PaxDb; Q6Z4K6; -.
DR PRIDE; Q6Z4K6; -.
DR EnsemblPlants; Os07t0202100-01; Os07t0202100-01; Os07g0202100.
DR GeneID; 4342669; -.
DR Gramene; Os07t0202100-01; Os07t0202100-01; Os07g0202100.
DR KEGG; osa:4342669; -.
DR eggNOG; KOG0335; Eukaryota.
DR InParanoid; Q6Z4K6; -.
DR OMA; NHGNSRL; -.
DR OrthoDB; 595675at2759; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000007752; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR ExpressionAtlas; Q6Z4K6; baseline and differential.
DR Genevisible; Q6Z4K6; OS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR CDD; cd17967; DEADc_DDX3_DDX4; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR044763; Ded1/Dbp1_DEADc.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW RNA-binding.
FT CHAIN 1..638
FT /note="DEAD-box ATP-dependent RNA helicase 52B"
FT /id="PRO_0000282451"
FT DOMAIN 200..384
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 411..562
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..129
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 565..638
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 169..197
FT /note="Q motif"
FT MOTIF 328..331
FT /note="DEAD box"
FT BINDING 213..220
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT CONFLICT 231
FT /note="I -> F (in Ref. 5; AK101736)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 638 AA; 67172 MW; 4B5B2438F37A5819 CRC64;
MRSSWADSAA NAEESAPAAA ANHGNSRLPR SSYVPPHLRG QAAPAAPAQA GALPSAAAAA
QPSVGQPGVV GGPRWAGIVN GGGGGGGGSV GGSRQGFGAG GRGGGGGGGG GAWNSRPGGW
DRRDREPDPF ANSEAAEVDF EGENTGINFE AYEDIPVETS GHDVPPPANT FAEIDLGDAL
NENIRRCKYV KPTPVQRYAI PISIAGRDLM ACAQTGSGKT AAFCFPIISG IMRSRPPPRS
RGSRTAYPLA LILSPTRELS VQIHEEARKF AYQTGVKVVV AYGGAPITQQ LRELERGVEI
LVATPGRLMD LLERARVSLQ MIKYLALDEA DRMLDMGFEP QIRKIVEQMD MPPRGERQTM
LFSATFPKEI QRMASDFLAD YIFLAVGRVG SSTDLIVQRV EFVLDADKRS YLMDLLHAQR
ANGTHGKQAL TLVFVETKRG ADALENWLYN NGFPATSIHG DRTQQEREYA LRSFKSGATP
ILVATDVAAR GLDIPHVAHV INFDLPNDID DYVHRIGRTG RAGKSGLATA FFNESNTPLA
RPLSELMQEA NQEVPQWLER YAARSSFGGG GGRNRRSGGG ARFGGRDFRR DRGSGGGGYG
GGGGGYGGGG YGGGGGGGGY GGGSSYGGGG QGFSSAWD
