RH53_ORYSJ
ID RH53_ORYSJ Reviewed; 602 AA.
AC Q0D8N0;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 2.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=DEAD-box ATP-dependent RNA helicase 53;
DE EC=3.6.4.13;
GN OrderedLocusNames=Os07g0143700, LOC_Os07g05050;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q0D8N0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0D8N0-2; Sequence=VSP_024164;
CC Name=3;
CC IsoId=Q0D8N0-3; Sequence=VSP_024165, VSP_024166;
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX21/DDX50
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF20793.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP008213; BAF20793.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014963; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK068871; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015644679.1; XM_015789193.1. [Q0D8N0-1]
DR AlphaFoldDB; Q0D8N0; -.
DR SMR; Q0D8N0; -.
DR STRING; 4530.OS07T0143700-01; -.
DR iPTMnet; Q0D8N0; -.
DR PaxDb; Q0D8N0; -.
DR PRIDE; Q0D8N0; -.
DR GeneID; 4342387; -.
DR KEGG; osa:4342387; -.
DR eggNOG; KOG0331; Eukaryota.
DR HOGENOM; CLU_910846_0_0_1; -.
DR InParanoid; Q0D8N0; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR Genevisible; Q0D8N0; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0000373; P:Group II intron splicing; IBA:GO_Central.
DR GO; GO:0006364; P:rRNA processing; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Helicase; Hydrolase; Nucleotide-binding;
KW Reference proteome; RNA-binding.
FT CHAIN 1..602
FT /note="DEAD-box ATP-dependent RNA helicase 53"
FT /id="PRO_0000282476"
FT DOMAIN 115..288
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 317..462
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 33..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 503..602
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 84..112
FT /note="Q motif"
FT MOTIF 236..239
FT /note="DEAD box"
FT BINDING 128..135
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT VAR_SEQ 324..340
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_024164"
FT VAR_SEQ 324..330
FT /note="EHANGGK -> VSTLLNV (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_024165"
FT VAR_SEQ 331..602
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_024166"
FT CONFLICT 1
FT /note="M -> V (in Ref. 4; AK068871)"
FT /evidence="ECO:0000305"
FT CONFLICT 88
FT /note="R -> S (in Ref. 4; AK068871)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 602 AA; 65127 MW; CACC7FB89F24BB4E CRC64;
MFSLLSRALC AASSSPAAPR GRSLLAALLS PSASPLDPCR GPAAPEPPRR RAFHGSPSPL
GFRSTPASWS SPEAGAAVGG DDGLEVARLG ISPWIVERLA ARGITRLFPI QRAVLDPAMQ
GKDMIGRART GTGKTLAFGI PIMDRILRHN EKNGSGRNPL AIILAPTREL ARQVEKEFKE
SAPLDSLCVY GGVPISHQMR ALNYGVDVVV GTPGRIIDLL RRGVLNLSEI QFVVLDEADQ
MLAVGFDEDV EVIMENLPQN RQSMLFSATM PSWIRKITSK YLKDPIIIDL VGDEDQKLPE
GISLYSIASE HYGKPSILGP LIKEHANGGK CIVFTQTKRE ADRLAYAMGR SYACQALHGD
ISQNQRERTL SGFRDGRFNI LVATDVAARG LDIPNVDLVI HYELPNTSEL FVHRSGRTAR
AGKKGSAILI YTNDQARAVR IIEQDIGCKF TELPKIEVAD EASDMFNVVR DNRSRLAGSP
RTGGSSFGRG GYGGFGEGRS RGFGDFDGFG SSPNRGGRSR DAGSRYGSGF GDFRRPSNAF
GRSSSKQPDG FGFGDFGEGN FSRNGNRRSR SFDDSGSTRY SRRPNGFGTS DFGRSGGFDD
SN
