RH5_ORYSJ
ID RH5_ORYSJ Reviewed; 512 AA.
AC Q6YS30; A0A0P0X5C0; Q7XXP9;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=DEAD-box ATP-dependent RNA helicase 5;
DE EC=3.6.4.13;
GN OrderedLocusNames=Os07g0301200, LOC_Os07g20580; ORFNames=B1114D08.16-1;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 105-512, AND SUBCELLULAR LOCATION.
RC TISSUE=Panicle;
RX PubMed=15659629; DOI=10.1105/tpc.104.028456;
RA Moriguchi K., Suzuki T., Ito Y., Yamazaki Y., Niwa Y., Kurata N.;
RT "Functional isolation of novel nuclear proteins showing a variety of
RT subnuclear localizations.";
RL Plant Cell 17:389-403(2005).
CC -!- FUNCTION: ATP-dependent RNA helicase required for 60S ribosomal subunit
CC synthesis. Involved in efficient pre-rRNA processing, predominantly at
CC site A3, which is necessary for the normal formation of 25S and 5.8S
CC rRNAs (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX5/DBP2
CC subfamily. {ECO:0000305}.
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DR EMBL; AP006529; BAC84904.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF21338.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT01081.1; -; Genomic_DNA.
DR EMBL; AB110202; BAC78594.1; -; mRNA.
DR RefSeq; XP_015647459.1; XM_015791973.1.
DR AlphaFoldDB; Q6YS30; -.
DR SMR; Q6YS30; -.
DR STRING; 4530.OS07T0301200-01; -.
DR iPTMnet; Q6YS30; -.
DR PaxDb; Q6YS30; -.
DR PRIDE; Q6YS30; -.
DR EnsemblPlants; Os07t0301200-01; Os07t0301200-01; Os07g0301200.
DR GeneID; 4342981; -.
DR Gramene; Os07t0301200-01; Os07t0301200-01; Os07g0301200.
DR KEGG; osa:4342981; -.
DR eggNOG; KOG0331; Eukaryota.
DR InParanoid; Q6YS30; -.
DR OMA; KKKTHDM; -.
DR OrthoDB; 471730at2759; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR ExpressionAtlas; Q6YS30; baseline and differential.
DR Genevisible; Q6YS30; OS.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR GO; GO:0006364; P:rRNA processing; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Coiled coil; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing.
FT CHAIN 1..512
FT /note="DEAD-box ATP-dependent RNA helicase 5"
FT /id="PRO_0000282453"
FT DOMAIN 123..300
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 333..476
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 45..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 14..45
FT /evidence="ECO:0000255"
FT MOTIF 94..120
FT /note="Q motif"
FT MOTIF 248..251
FT /note="DEAD box"
FT COMPBIAS 51..66
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 136..143
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 512 AA; 55623 MW; F7A79AF0D5BAA330 CRC64;
MGRSMLPEQQ EDVSRKSKKE KKSKKDKKRK LEAEAEVVVV EAAAATSTDE ATKSSKKKRA
KGDLGQGEEA ENGGGKVVAV TGKGSADAKY APLSSFAATA LPPQVLDCCK GFERPSPIQA
YAWPYLLDGR DFIGIAATGS GKTIAFGVPA LMHVRRKMGE KSAKKGVPRV LVLSPTRELA
QQIADVLCEA GAPCGISSVC LYGGTSKGPQ ISALKSGVDI VIGTPGRMKD LIEMGICRLN
DVSFVVLDEA DRMLDMGFEP EVRAILSQTA SVRQTVMFSA TWPPAVHQLA QEFMDPNPIK
VVIGSEDLAA NHDVMQIVEV LDDRSRDSRL VALLDKYHKA QRNRVLVFVL YKREATRVET
MLQRRGWSAV SVHGDKAQHD RTKALSLFKE GSCPLMIATD VASRGLDIPD VEVVINYSYP
LTTEDYVHRI GRTGRAGKKG VAHTFFTQEN KGLAGELVNV LREAGQVVPP ALTKFGTHVK
KKESQIYGSH FKEIKADAPK STKITFGDSD ED
