ID   RHAM_RHILT              Reviewed;         106 AA.
AC   Q7BSH1;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=L-rhamnose mutarotase;
DE            EC=5.1.3.32 {ECO:0000269|PubMed:18156270};
DE   AltName: Full=Rhamnose 1-epimerase;
DE   AltName: Full=Type-3 mutarotase;
GN   Name=rhaM; Synonyms=rhaU;
OS   Rhizobium leguminosarum bv. trifolii.
OG   Plasmid pRleW14-2c.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=386;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PATHWAY.
RC   STRAIN=Rlt100;
RX   PubMed=15576793; DOI=10.1128/jb.186.24.8433-8442.2004;
RA   Richardson J.S., Hynes M.F., Oresnik I.J.;
RT   "A genetic locus necessary for rhamnose uptake and catabolism in Rhizobium
RT   leguminosarum bv. trifolii.";
RL   J. Bacteriol. 186:8433-8442(2004).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) IN COMPLEX WITH L-RHAMNOSE, FUNCTION,
RP   CATALYTIC ACTIVITY, ACTIVE SITE, REACTION MECHANISM, SUBUNIT, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=Rlt100;
RX   PubMed=18156270; DOI=10.1128/jb.01120-07;
RA   Richardson J.S., Carpena X., Switala J., Perez-Luque R., Donald L.J.,
RA   Loewen P.C., Oresnik I.J.;
RT   "RhaU of Rhizobium leguminosarum is a rhamnose mutarotase.";
RL   J. Bacteriol. 190:2903-2910(2008).
CC   -!- FUNCTION: Involved in the anomeric conversion of L-rhamnose.
CC       {ECO:0000269|PubMed:18156270}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-L-rhamnose = beta-L-rhamnose; Xref=Rhea:RHEA:25584,
CC         ChEBI:CHEBI:27586, ChEBI:CHEBI:27907; EC=5.1.3.32;
CC         Evidence={ECO:0000269|PubMed:18156270};
CC   -!- PATHWAY: Carbohydrate degradation; L-rhamnose degradation.
CC       {ECO:0000269|PubMed:15576793}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:18156270}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the rhamnose mutarotase family. {ECO:0000305}.
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DR   EMBL; AF085687; AAQ92411.1; -; Genomic_DNA.
DR   PDB; 2QLW; X-ray; 1.60 A; A/B=2-106.
DR   PDB; 2QLX; X-ray; 2.00 A; A/B=1-106.
DR   PDBsum; 2QLW; -.
DR   PDBsum; 2QLX; -.
DR   AlphaFoldDB; Q7BSH1; -.
DR   SMR; Q7BSH1; -.
DR   BioCyc; MetaCyc:MON-18536; -.
DR   BRENDA; 5.1.3.32; 5343.
DR   UniPathway; UPA00541; -.
DR   EvolutionaryTrace; Q7BSH1; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0062192; F:L-rhamnose mutarotase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019301; P:rhamnose catabolic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_01663; L_rham_rotase; 1.
DR   InterPro; IPR011008; Dimeric_a/b-barrel.
DR   InterPro; IPR013448; L-rhamnose_mutarotase.
DR   InterPro; IPR008000; Rham/fucose_mutarotase.
DR   Pfam; PF05336; rhaM; 1.
DR   SUPFAM; SSF54909; SSF54909; 1.
DR   TIGRFAMs; TIGR02625; YiiL_rotase; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Carbohydrate metabolism; Cytoplasm; Isomerase; Plasmid;
KW   Rhamnose metabolism.
FT   CHAIN           1..106
FT                   /note="L-rhamnose mutarotase"
FT                   /id="PRO_0000344593"
FT   ACT_SITE        24
FT                   /note="Proton donor"
FT   BINDING         20
FT                   /ligand="substrate"
FT   BINDING         43
FT                   /ligand="substrate"
FT   BINDING         78..79
FT                   /ligand="substrate"
FT   STRAND          3..12
FT                   /evidence="ECO:0007829|PDB:2QLW"
FT   HELIX           17..25
FT                   /evidence="ECO:0007829|PDB:2QLW"
FT   HELIX           29..38
FT                   /evidence="ECO:0007829|PDB:2QLW"
FT   STRAND          41..48
FT                   /evidence="ECO:0007829|PDB:2QLW"
FT   TURN            49..52
FT                   /evidence="ECO:0007829|PDB:2QLW"
FT   STRAND          53..61
FT                   /evidence="ECO:0007829|PDB:2QLW"
FT   HELIX           66..71
FT                   /evidence="ECO:0007829|PDB:2QLW"
FT   HELIX           73..82
FT                   /evidence="ECO:0007829|PDB:2QLW"
FT   HELIX           83..85
FT                   /evidence="ECO:0007829|PDB:2QLW"
FT   STRAND          100..105
FT                   /evidence="ECO:0007829|PDB:2QLW"
SQ   SEQUENCE   106 AA;  12292 MW;  4DE860C3A622C7F5 CRC64;
     MTLEKHAFKM QLNPGMEAEY RKRHDEIWPE LVDLLHQSGA SDYSIHLDRE TNTLFGVLTR
     PKDHTMASLP DHPVMKKWWA HMADIMATNP DNSPVQSDLV TLFHMP