RHAR_ECO57
ID RHAR_ECO57 Reviewed; 282 AA.
AC Q8X7B3; Q7A995;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=HTH-type transcriptional activator RhaR {ECO:0000255|HAMAP-Rule:MF_01533};
DE AltName: Full=L-rhamnose operon transcriptional activator RhaR {ECO:0000255|HAMAP-Rule:MF_01533};
GN Name=rhaR {ECO:0000255|HAMAP-Rule:MF_01533};
GN OrderedLocusNames=Z5450, ECs4833;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Activates expression of the rhaSR operon in response to L-
CC rhamnose. {ECO:0000255|HAMAP-Rule:MF_01533}.
CC -!- SUBUNIT: Binds DNA as a dimer. {ECO:0000255|HAMAP-Rule:MF_01533}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01533}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG59100.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAB38256.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE005174; AAG59100.1; ALT_INIT; Genomic_DNA.
DR EMBL; BA000007; BAB38256.1; ALT_INIT; Genomic_DNA.
DR PIR; A98233; A98233.
DR PIR; H86079; H86079.
DR RefSeq; NP_312860.2; NC_002695.1.
DR RefSeq; WP_001302243.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; Q8X7B3; -.
DR SMR; Q8X7B3; -.
DR STRING; 155864.EDL933_5233; -.
DR EnsemblBacteria; AAG59100; AAG59100; Z5450.
DR EnsemblBacteria; BAB38256; BAB38256; ECs_4833.
DR GeneID; 915065; -.
DR KEGG; ece:Z5450; -.
DR KEGG; ecs:ECs_4833; -.
DR PATRIC; fig|386585.9.peg.5051; -.
DR eggNOG; COG1917; Bacteria.
DR eggNOG; COG4977; Bacteria.
DR HOGENOM; CLU_000445_88_5_6; -.
DR OMA; NREVGMT; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR GO; GO:0019299; P:rhamnose metabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 2.60.120.10; -; 1.
DR HAMAP; MF_01533; HTH_type_RhaR; 1.
DR InterPro; IPR003313; AraC-bd.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR InterPro; IPR023699; Tscrpt_act_RhaR.
DR InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR Pfam; PF02311; AraC_binding; 1.
DR Pfam; PF12833; HTH_18; 1.
DR PRINTS; PR00032; HTHARAC.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; SSF46689; 2.
DR SUPFAM; SSF51182; SSF51182; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 3: Inferred from homology;
KW Activator; Cytoplasm; DNA-binding; Reference proteome; Repeat;
KW Rhamnose metabolism; Transcription; Transcription regulation.
FT CHAIN 1..282
FT /note="HTH-type transcriptional activator RhaR"
FT /id="PRO_0000194553"
FT DOMAIN 179..277
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01533"
FT DNA_BIND 196..217
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01533"
FT DNA_BIND 244..267
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01533"
FT SITE 246
FT /note="Interaction with sigma-70"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01533"
SQ SEQUENCE 282 AA; 32346 MW; F78121C953BA38E8 CRC64;
MAHQLKLLKD DFFASDQQAV AVADRYPQDV FAEHTHDFCE LVIVWRGNGL HVLNDRPYRI
TRGDLFYIHA DDKHSYASVN DLVLQNIIYC PERLKLNLDW QGAIPGFSAS AGQPHWRLGS
MGMAQARQVI GQLEHESSQH VPFANEMAEL LFGQLVMLLN RHRYTSDSLP PTSSETLLDK
LITRLAASLK SPFALDKFCD EASCSERVLR QQFRQQTGMT INQYLRQVRV CHAQYLLQHS
RLLISDISTE CGFEDSNYFS VVFTRETGMT PSQWRHLNSQ KD
