RHAR_ECOBW
ID RHAR_ECOBW Reviewed; 282 AA.
AC C5A074;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 1.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=HTH-type transcriptional activator RhaR {ECO:0000255|HAMAP-Rule:MF_01533};
DE AltName: Full=L-rhamnose operon transcriptional activator RhaR {ECO:0000255|HAMAP-Rule:MF_01533};
GN Name=rhaR {ECO:0000255|HAMAP-Rule:MF_01533}; OrderedLocusNames=BWG_3576;
OS Escherichia coli (strain K12 / MC4100 / BW2952).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=595496;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MC4100 / BW2952;
RX PubMed=19376874; DOI=10.1128/jb.00118-09;
RA Ferenci T., Zhou Z., Betteridge T., Ren Y., Liu Y., Feng L., Reeves P.R.,
RA Wang L.;
RT "Genomic sequencing reveals regulatory mutations and recombinational events
RT in the widely used MC4100 lineage of Escherichia coli K-12.";
RL J. Bacteriol. 191:4025-4029(2009).
CC -!- FUNCTION: Activates expression of the rhaSR operon in response to L-
CC rhamnose. {ECO:0000255|HAMAP-Rule:MF_01533}.
CC -!- SUBUNIT: Binds DNA as a dimer. {ECO:0000255|HAMAP-Rule:MF_01533}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01533}.
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DR EMBL; CP001396; ACR62908.1; -; Genomic_DNA.
DR RefSeq; WP_001336056.1; NC_012759.1.
DR AlphaFoldDB; C5A074; -.
DR SMR; C5A074; -.
DR KEGG; ebw:BWG_3576; -.
DR HOGENOM; CLU_000445_88_5_6; -.
DR OMA; NREVGMT; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR GO; GO:0019299; P:rhamnose metabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 2.60.120.10; -; 1.
DR HAMAP; MF_01533; HTH_type_RhaR; 1.
DR InterPro; IPR003313; AraC-bd.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR InterPro; IPR023699; Tscrpt_act_RhaR.
DR InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR Pfam; PF02311; AraC_binding; 1.
DR Pfam; PF12833; HTH_18; 1.
DR PRINTS; PR00032; HTHARAC.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; SSF46689; 2.
DR SUPFAM; SSF51182; SSF51182; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 3: Inferred from homology;
KW Activator; Cytoplasm; DNA-binding; Repeat; Rhamnose metabolism;
KW Transcription; Transcription regulation.
FT CHAIN 1..282
FT /note="HTH-type transcriptional activator RhaR"
FT /id="PRO_1000215402"
FT DOMAIN 179..277
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01533"
FT DNA_BIND 196..217
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01533"
FT DNA_BIND 244..267
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01533"
FT SITE 246
FT /note="Interaction with sigma-70"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01533"
SQ SEQUENCE 282 AA; 32373 MW; 5563D7B0773CEC9D CRC64;
MAHQLKLLKD DFFASDQQAV AVADRYPQDV FAEHTHDFCE LVIVWRGNGL HVLNDRPYRI
TRGDLFYIHA DDKHSYASVN DLVLQNIIYC PERLKLNLDW QGAIPGFNAS AGQPHWRLGS
MGMAQARQVI GQLEHESSQH VPFANEMAEL LFGQLVMLLN RHRYTSDSLP PTSSETLLDK
LITRLAASLK SPFALDKFCD EASCSERVLR QQFRQQTGMT INQYLRQVRV CHAQYLLQHS
RLLISDISTE CGFEDSNYFS VVFTRETGMT PSQWRHLNSQ KD
