ID   RHAR_PECAS              Reviewed;         284 AA.
AC   Q6DA21;
DT   12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 114.
DE   RecName: Full=HTH-type transcriptional activator RhaR {ECO:0000255|HAMAP-Rule:MF_01533};
DE   AltName: Full=L-rhamnose operon transcriptional activator RhaR {ECO:0000255|HAMAP-Rule:MF_01533};
GN   Name=rhaR {ECO:0000255|HAMAP-Rule:MF_01533}; OrderedLocusNames=ECA0442;
OS   Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia
OS   carotovora subsp. atroseptica).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Pectobacterium.
OX   NCBI_TaxID=218491;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SCRI 1043 / ATCC BAA-672;
RX   PubMed=15263089; DOI=10.1073/pnas.0402424101;
RA   Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J.,
RA   Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K.,
RA   Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J.,
RA   Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Norbertczak H.,
RA   Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S.,
RA   Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K.;
RT   "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora
RT   subsp. atroseptica and characterization of virulence factors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004).
CC   -!- FUNCTION: Activates expression of the rhaSR operon in response to L-
CC       rhamnose. {ECO:0000255|HAMAP-Rule:MF_01533}.
CC   -!- SUBUNIT: Binds DNA as a dimer. {ECO:0000255|HAMAP-Rule:MF_01533}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01533}.
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DR   EMBL; BX950851; CAG73357.1; -; Genomic_DNA.
DR   RefSeq; WP_011092064.1; NC_004547.2.
DR   AlphaFoldDB; Q6DA21; -.
DR   SMR; Q6DA21; -.
DR   STRING; 218491.ECA0442; -.
DR   DNASU; 2884602; -.
DR   EnsemblBacteria; CAG73357; CAG73357; ECA0442.
DR   KEGG; eca:ECA0442; -.
DR   PATRIC; fig|218491.5.peg.447; -.
DR   eggNOG; COG1917; Bacteria.
DR   eggNOG; COG4977; Bacteria.
DR   HOGENOM; CLU_000445_88_5_6; -.
DR   OMA; NREVGMT; -.
DR   OrthoDB; 1268974at2; -.
DR   Proteomes; UP000007966; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   GO; GO:0019299; P:rhamnose metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.60.120.10; -; 1.
DR   HAMAP; MF_01533; HTH_type_RhaR; 1.
DR   InterPro; IPR003313; AraC-bd.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   InterPro; IPR023699; Tscrpt_act_RhaR.
DR   InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR   Pfam; PF02311; AraC_binding; 1.
DR   Pfam; PF12833; HTH_18; 1.
DR   PRINTS; PR00032; HTHARAC.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 2.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Reference proteome; Repeat;
KW   Rhamnose metabolism; Transcription; Transcription regulation.
FT   CHAIN           1..284
FT                   /note="HTH-type transcriptional activator RhaR"
FT                   /id="PRO_0000194554"
FT   DOMAIN          181..279
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01533"
FT   DNA_BIND        198..219
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01533"
FT   DNA_BIND        246..269
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01533"
FT   SITE            248
FT                   /note="Interaction with sigma-70"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01533"
SQ   SEQUENCE   284 AA;  32761 MW;  1440E4FE10C039B4 CRC64;
     MATAIRGLKL QTEDYFLTDK NAVMVAERHP QPVFPLHHHD FDELVIVWRG NGLHLWNDVP
     YRITRGDMFY VSAHDRHSYE SVHELELDNI LYIRNRLTLS ADWQTLLPSG ELPQSQRHWC
     LGSEGMDTIR EKVDALTQEC MKSDALSLQL SEALLLQIAL LAARYRHSPD SPQLADAHQL
     DMLMNALRAS IAAPFRFEAF CEQHHFSARS LRSRFKEQTG MSVPHYLRQL RLCKAMELLR
     YDLQTIGDVA ALCGFEDSNY FSVVFHQAFG VSPSAYRQRF LNVE