ID   RHAS_ECO7I              Reviewed;         278 AA.
AC   B7NUA1;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=HTH-type transcriptional activator RhaS {ECO:0000255|HAMAP-Rule:MF_01534};
DE   AltName: Full=L-rhamnose operon regulatory protein RhaS {ECO:0000255|HAMAP-Rule:MF_01534};
GN   Name=rhaS {ECO:0000255|HAMAP-Rule:MF_01534};
GN   OrderedLocusNames=ECIAI39_3090;
OS   Escherichia coli O7:K1 (strain IAI39 / ExPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=585057;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IAI39 / ExPEC;
RX   PubMed=19165319; DOI=10.1371/journal.pgen.1000344;
RA   Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P.,
RA   Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H.,
RA   Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E.,
RA   Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C.,
RA   Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S.,
RA   Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J.,
RA   Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E.;
RT   "Organised genome dynamics in the Escherichia coli species results in
RT   highly diverse adaptive paths.";
RL   PLoS Genet. 5:E1000344-E1000344(2009).
CC   -!- FUNCTION: Activates expression of the rhaBAD and rhaT operons.
CC       {ECO:0000255|HAMAP-Rule:MF_01534}.
CC   -!- SUBUNIT: Binds DNA as a dimer. {ECO:0000255|HAMAP-Rule:MF_01534}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01534}.
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DR   EMBL; CU928164; CAR19209.1; -; Genomic_DNA.
DR   RefSeq; WP_000217149.1; NC_011750.1.
DR   RefSeq; YP_002409020.1; NC_011750.1.
DR   AlphaFoldDB; B7NUA1; -.
DR   SMR; B7NUA1; -.
DR   STRING; 585057.ECIAI39_3090; -.
DR   EnsemblBacteria; CAR19209; CAR19209; ECIAI39_3090.
DR   KEGG; ect:ECIAI39_3090; -.
DR   PATRIC; fig|585057.6.peg.3204; -.
DR   HOGENOM; CLU_000445_88_5_6; -.
DR   OMA; GHYPSHW; -.
DR   Proteomes; UP000000749; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   GO; GO:0019299; P:rhamnose metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.60.120.10; -; 1.
DR   HAMAP; MF_01534; HTH_type_RhaS; 1.
DR   InterPro; IPR003313; AraC-bd.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR037923; HTH-like.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR018062; HTH_AraC-typ_CS.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR   InterPro; IPR023609; Tscrpt_reg_HTH_RhaS.
DR   Pfam; PF02311; AraC_binding; 1.
DR   Pfam; PF12833; HTH_18; 1.
DR   PRINTS; PR00032; HTHARAC.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 2.
DR   SUPFAM; SSF51215; SSF51215; 1.
DR   PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Repeat; Rhamnose metabolism;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..278
FT                   /note="HTH-type transcriptional activator RhaS"
FT                   /id="PRO_1000200954"
FT   DOMAIN          174..272
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT   DNA_BIND        191..212
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT   DNA_BIND        239..262
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT   SITE            241
FT                   /note="Interaction with sigma-70"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT   SITE            250
FT                   /note="Interaction with sigma-70"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
SQ   SEQUENCE   278 AA;  32402 MW;  71E981876A6F21A9 CRC64;
     MTVLHSVDFF PSGNASVAIE PRLPQADFPE HHHDFHEIVI VEHGTGIHVF NGQPYTITGG
     TVCFVRDHDR HLYEHTDNLC LTNVLYRSPD RFQFLAGLNQ LLPQEQDGQY PSHWRVNHSV
     LQQVRQLVAQ MEQQEEENDL PSTASREILF MQLLLLLRKS SLQENLENSA SRLNLLLAWL
     EDHFADEVNW DAVADQFSLS LRTLHRQLKQ KTGLTPQRYL NRLRLMKARH LLRHSEASVT
     DIAYRCGFSD SNHFSTLFRR EFNWSPRDIR QGRDGFLQ