RHAS_ECOSE
ID RHAS_ECOSE Reviewed; 278 AA.
AC B6I4P8;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 1.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=HTH-type transcriptional activator RhaS {ECO:0000255|HAMAP-Rule:MF_01534};
DE AltName: Full=L-rhamnose operon regulatory protein RhaS {ECO:0000255|HAMAP-Rule:MF_01534};
GN Name=rhaS {ECO:0000255|HAMAP-Rule:MF_01534}; OrderedLocusNames=ECSE_4194;
OS Escherichia coli (strain SE11).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=409438;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SE11;
RX PubMed=18931093; DOI=10.1093/dnares/dsn026;
RA Oshima K., Toh H., Ogura Y., Sasamoto H., Morita H., Park S.-H., Ooka T.,
RA Iyoda S., Taylor T.D., Hayashi T., Itoh K., Hattori M.;
RT "Complete genome sequence and comparative analysis of the wild-type
RT commensal Escherichia coli strain SE11 isolated from a healthy adult.";
RL DNA Res. 15:375-386(2008).
CC -!- FUNCTION: Activates expression of the rhaBAD and rhaT operons.
CC {ECO:0000255|HAMAP-Rule:MF_01534}.
CC -!- SUBUNIT: Binds DNA as a dimer. {ECO:0000255|HAMAP-Rule:MF_01534}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01534}.
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DR EMBL; AP009240; BAG79718.1; -; Genomic_DNA.
DR RefSeq; WP_000217139.1; NC_011415.1.
DR AlphaFoldDB; B6I4P8; -.
DR SMR; B6I4P8; -.
DR EnsemblBacteria; BAG79718; BAG79718; ECSE_4194.
DR KEGG; ecy:ECSE_4194; -.
DR HOGENOM; CLU_000445_88_5_6; -.
DR OMA; GHYPSHW; -.
DR Proteomes; UP000008199; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR GO; GO:0019299; P:rhamnose metabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 2.60.120.10; -; 1.
DR HAMAP; MF_01534; HTH_type_RhaS; 1.
DR InterPro; IPR003313; AraC-bd.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR037923; HTH-like.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR InterPro; IPR023609; Tscrpt_reg_HTH_RhaS.
DR Pfam; PF02311; AraC_binding; 1.
DR Pfam; PF12833; HTH_18; 1.
DR PRINTS; PR00032; HTHARAC.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; SSF46689; 2.
DR SUPFAM; SSF51215; SSF51215; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 3: Inferred from homology;
KW Activator; Cytoplasm; DNA-binding; Repeat; Rhamnose metabolism;
KW Transcription; Transcription regulation.
FT CHAIN 1..278
FT /note="HTH-type transcriptional activator RhaS"
FT /id="PRO_1000200949"
FT DOMAIN 174..272
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT DNA_BIND 191..212
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT DNA_BIND 239..262
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT SITE 241
FT /note="Interaction with sigma-70"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT SITE 250
FT /note="Interaction with sigma-70"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
SQ SEQUENCE 278 AA; 32329 MW; 8537451592E77CF0 CRC64;
MTVLHSVDFF PSGNASVAIE PRLPQADFPE HHHDFHEIVI VEHGTGIHVF NGQPYTITGG
TVCFVRDHDR HLYEHTDNLC LTNVLYRSPD RFQFLAGLNQ LLPQELDGQY PSHWRVNHSV
LQQVRQLVAQ MEQQEGENDL PSTASREILF MQLLLLLRKS SLQENLENSA SRLNLLLAWL
EDHFADEVNW DAVAEQFSLS LRTLHRQLKQ QTGLTPQRYL NRLRLMKARH LLRHSEASVT
DIAYRCGFSD SNHFSTLFRR EFNWSPRDIR QGRDGFLQ
