ID   RHAS_ESCF3              Reviewed;         278 AA.
AC   B7LVD8;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=HTH-type transcriptional activator RhaS {ECO:0000255|HAMAP-Rule:MF_01534};
DE   AltName: Full=L-rhamnose operon regulatory protein RhaS {ECO:0000255|HAMAP-Rule:MF_01534};
GN   Name=rhaS {ECO:0000255|HAMAP-Rule:MF_01534}; OrderedLocusNames=EFER_3866;
OS   Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CCUG 18766 / IAM
OS   14443 / JCM 21226 / LMG 7866 / NBRC 102419 / NCTC 12128 / CDC 0568-73).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=585054;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35469 / DSM 13698 / BCRC 15582 / CCUG 18766 / IAM 14443 / JCM
RC   21226 / LMG 7866 / NBRC 102419 / NCTC 12128 / CDC 0568-73;
RX   PubMed=19165319; DOI=10.1371/journal.pgen.1000344;
RA   Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P.,
RA   Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H.,
RA   Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E.,
RA   Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C.,
RA   Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S.,
RA   Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J.,
RA   Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E.;
RT   "Organised genome dynamics in the Escherichia coli species results in
RT   highly diverse adaptive paths.";
RL   PLoS Genet. 5:E1000344-E1000344(2009).
CC   -!- FUNCTION: Activates expression of the rhaBAD and rhaT operons.
CC       {ECO:0000255|HAMAP-Rule:MF_01534}.
CC   -!- SUBUNIT: Binds DNA as a dimer. {ECO:0000255|HAMAP-Rule:MF_01534}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01534}.
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DR   EMBL; CU928158; CAQ91301.1; -; Genomic_DNA.
DR   RefSeq; WP_000163649.1; NC_011740.1.
DR   AlphaFoldDB; B7LVD8; -.
DR   SMR; B7LVD8; -.
DR   PRIDE; B7LVD8; -.
DR   EnsemblBacteria; CAQ91301; CAQ91301; EFER_3866.
DR   KEGG; efe:EFER_3866; -.
DR   HOGENOM; CLU_000445_88_5_6; -.
DR   OMA; GHYPSHW; -.
DR   OrthoDB; 1268974at2; -.
DR   BioCyc; EFER585054:EFER_RS19315-MON; -.
DR   Proteomes; UP000000745; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   GO; GO:0019299; P:rhamnose metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.60.120.10; -; 1.
DR   HAMAP; MF_01534; HTH_type_RhaS; 1.
DR   InterPro; IPR003313; AraC-bd.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR037923; HTH-like.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR   InterPro; IPR023609; Tscrpt_reg_HTH_RhaS.
DR   Pfam; PF02311; AraC_binding; 1.
DR   Pfam; PF12833; HTH_18; 1.
DR   PRINTS; PR00032; HTHARAC.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 2.
DR   SUPFAM; SSF51215; SSF51215; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Repeat; Rhamnose metabolism;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..278
FT                   /note="HTH-type transcriptional activator RhaS"
FT                   /id="PRO_1000200956"
FT   DOMAIN          174..272
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT   DNA_BIND        191..212
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT   DNA_BIND        239..262
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT   SITE            250
FT                   /note="Interaction with sigma-70"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
SQ   SEQUENCE   278 AA;  32181 MW;  43E252A5BEDE36C6 CRC64;
     MTKLHSVDFF PAQEVSVAIE PRLPQGAFPE HHHDFHEIVI VEHGTGIHVL NGQPYTISGG
     MVCFIRDSDR HMYEHTDNLN LTNVLYRSPD KFRFLAGLHQ LLPQECNGHY PSHWRIDQHT
     LQQVRQLITR LEQVSAGQDL STVANREILF MQLLVALRKN SLLEGDENTD ARLNHLIAWL
     EDHFAEAVSW EAIADRFALS LRTLHRQLKQ HTGLTPQRYL NRLRLVKARH LLRHSDDSVT
     HIAFSCGFAD SNHFSTLFRR EFGWSPREIR QGRDASLQ