ID   RHAS_KLEP3              Reviewed;         278 AA.
AC   B5XZ49;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=HTH-type transcriptional activator RhaS {ECO:0000255|HAMAP-Rule:MF_01534};
DE   AltName: Full=L-rhamnose operon regulatory protein RhaS {ECO:0000255|HAMAP-Rule:MF_01534};
GN   Name=rhaS {ECO:0000255|HAMAP-Rule:MF_01534}; OrderedLocusNames=KPK_5465;
OS   Klebsiella pneumoniae (strain 342).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=507522;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=342;
RX   PubMed=18654632; DOI=10.1371/journal.pgen.1000141;
RA   Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H.,
RA   Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y.,
RA   Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W.,
RA   Methe B.A.;
RT   "Complete genome sequence of the N2-fixing broad host range endophyte
RT   Klebsiella pneumoniae 342 and virulence predictions verified in mice.";
RL   PLoS Genet. 4:E1000141-E1000141(2008).
CC   -!- FUNCTION: Activates expression of the rhaBAD and rhaT operons.
CC       {ECO:0000255|HAMAP-Rule:MF_01534}.
CC   -!- SUBUNIT: Binds DNA as a dimer. {ECO:0000255|HAMAP-Rule:MF_01534}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01534}.
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DR   EMBL; CP000964; ACI10038.1; -; Genomic_DNA.
DR   AlphaFoldDB; B5XZ49; -.
DR   SMR; B5XZ49; -.
DR   EnsemblBacteria; ACI10038; ACI10038; KPK_5465.
DR   KEGG; kpe:KPK_5465; -.
DR   HOGENOM; CLU_000445_88_5_6; -.
DR   OMA; GHYPSHW; -.
DR   OrthoDB; 1268974at2; -.
DR   Proteomes; UP000001734; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   GO; GO:0019299; P:rhamnose metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.60.120.10; -; 1.
DR   HAMAP; MF_01534; HTH_type_RhaS; 1.
DR   InterPro; IPR003313; AraC-bd.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR037923; HTH-like.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR018062; HTH_AraC-typ_CS.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR   InterPro; IPR023609; Tscrpt_reg_HTH_RhaS.
DR   Pfam; PF02311; AraC_binding; 1.
DR   Pfam; PF12833; HTH_18; 1.
DR   PRINTS; PR00032; HTHARAC.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 2.
DR   SUPFAM; SSF51215; SSF51215; 1.
DR   PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Repeat; Rhamnose metabolism;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..278
FT                   /note="HTH-type transcriptional activator RhaS"
FT                   /id="PRO_1000200957"
FT   DOMAIN          174..272
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT   DNA_BIND        191..212
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT   DNA_BIND        239..262
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT   SITE            241
FT                   /note="Interaction with sigma-70"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT   SITE            250
FT                   /note="Interaction with sigma-70"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
SQ   SEQUENCE   278 AA;  32063 MW;  59D4A7AC7366F322 CRC64;
     MTILHSTDFF KAGISTVAIE PRLPQSAFPE HHHDFHEIVI VEQGTGIHVF NGQPYTIGGG
     SVCFIRDHDR HLYEHTDNLC LTNVLYRAPD AFRFLAGVSQ LLPQEQEGNY PSHWRVNQTV
     LQQVRHIVAQ MEAMGSETDT HAVASREILF MQLLVLLRKS SLAEEATNND ARLNQLLAWL
     EDHFAQEICW EEVAAQFSLS LRTLHRQLKQ QTGLTPQRYL NRVRLMKARH LLRHSDESVT
     DIAFRCGFGD SNHFSTLFRR EFDWSPRDIR QGRDAILQ