ID   RHAS_PECAS              Reviewed;         279 AA.
AC   Q6DA22;
DT   12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=HTH-type transcriptional activator RhaS {ECO:0000255|HAMAP-Rule:MF_01534};
DE   AltName: Full=L-rhamnose operon regulatory protein RhaS {ECO:0000255|HAMAP-Rule:MF_01534};
GN   Name=rhaS {ECO:0000255|HAMAP-Rule:MF_01534}; OrderedLocusNames=ECA0441;
OS   Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia
OS   carotovora subsp. atroseptica).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Pectobacterium.
OX   NCBI_TaxID=218491;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SCRI 1043 / ATCC BAA-672;
RX   PubMed=15263089; DOI=10.1073/pnas.0402424101;
RA   Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J.,
RA   Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K.,
RA   Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J.,
RA   Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Norbertczak H.,
RA   Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S.,
RA   Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K.;
RT   "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora
RT   subsp. atroseptica and characterization of virulence factors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004).
CC   -!- FUNCTION: Activates expression of the rhaBAD and rhaT operons.
CC       {ECO:0000255|HAMAP-Rule:MF_01534}.
CC   -!- SUBUNIT: Binds DNA as a dimer. {ECO:0000255|HAMAP-Rule:MF_01534}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01534}.
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DR   EMBL; BX950851; CAG73356.1; -; Genomic_DNA.
DR   RefSeq; WP_011092063.1; NC_004547.2.
DR   AlphaFoldDB; Q6DA22; -.
DR   SMR; Q6DA22; -.
DR   STRING; 218491.ECA0441; -.
DR   DNASU; 2884603; -.
DR   EnsemblBacteria; CAG73356; CAG73356; ECA0441.
DR   GeneID; 57207295; -.
DR   KEGG; eca:ECA0441; -.
DR   PATRIC; fig|218491.5.peg.446; -.
DR   eggNOG; COG1917; Bacteria.
DR   eggNOG; COG2169; Bacteria.
DR   HOGENOM; CLU_000445_88_5_6; -.
DR   OMA; GHYPSHW; -.
DR   OrthoDB; 1268974at2; -.
DR   Proteomes; UP000007966; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   GO; GO:0019299; P:rhamnose metabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.60.120.10; -; 1.
DR   HAMAP; MF_01534; HTH_type_RhaS; 1.
DR   InterPro; IPR003313; AraC-bd.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR037923; HTH-like.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR018062; HTH_AraC-typ_CS.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR   InterPro; IPR023609; Tscrpt_reg_HTH_RhaS.
DR   Pfam; PF02311; AraC_binding; 1.
DR   Pfam; PF12833; HTH_18; 1.
DR   PRINTS; PR00032; HTHARAC.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 2.
DR   SUPFAM; SSF51215; SSF51215; 1.
DR   PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Reference proteome; Repeat;
KW   Rhamnose metabolism; Transcription; Transcription regulation.
FT   CHAIN           1..279
FT                   /note="HTH-type transcriptional activator RhaS"
FT                   /id="PRO_0000194566"
FT   DOMAIN          175..273
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT   DNA_BIND        192..213
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT   DNA_BIND        240..263
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT   SITE            251
FT                   /note="Interaction with sigma-70"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
SQ   SEQUENCE   279 AA;  31707 MW;  410C2BDA187E1411 CRC64;
     MTLLRGDDFF TSRAVTVAVE PRTPQTAFPE HYHDFWEIVL VEQGAGVHVF NDQPYALCSG
     SVFFVRDNDR HLFEDVEGLC LTNMLYRSPR GFRFLSDIAA FLPYGPNGEW QGQWQVNAAG
     MQQLKQSLNS LAGLAQSDAP EAIAASESLF LQILVQLRQH CFQTEGNGSE RQGVQALLGW
     LQNNYSEEVN WGGLADQFSL PLRTLHRQLK QHTGMTPQRY LNRLRLLEAR RRLQQSDDSI
     TTIAHACGFS DSNHFSTQFR KAFSQAPKSL RHQAFSREE