RHAS_SALTY
ID RHAS_SALTY Reviewed; 278 AA.
AC P0A2S9; P27029;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=HTH-type transcriptional activator RhaS {ECO:0000255|HAMAP-Rule:MF_01534};
DE AltName: Full=L-rhamnose operon regulatory protein RhaS {ECO:0000255|HAMAP-Rule:MF_01534};
GN Name=rhaS {ECO:0000255|HAMAP-Rule:MF_01534}; Synonyms=rhaC2;
GN OrderedLocusNames=STM4048;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RX PubMed=1657713; DOI=10.1016/0378-1119(91)90511-9;
RA Nishitani J., Wilcox G.;
RT "Cloning and characterization of the L-rhamnose regulon in Salmonella
RT typhimurium LT2.";
RL Gene 105:37-42(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Activates expression of the rhaBAD and rhaT operons.
CC {ECO:0000255|HAMAP-Rule:MF_01534}.
CC -!- SUBUNIT: Binds DNA as a dimer. {ECO:0000255|HAMAP-Rule:MF_01534}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01534}.
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DR EMBL; X57299; CAA40556.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL22888.1; -; Genomic_DNA.
DR PIR; JQ1288; JQ1288.
DR RefSeq; NP_462929.1; NC_003197.2.
DR RefSeq; WP_000217112.1; NC_003197.2.
DR AlphaFoldDB; P0A2S9; -.
DR SMR; P0A2S9; -.
DR STRING; 99287.STM4048; -.
DR PaxDb; P0A2S9; -.
DR DNASU; 1255575; -.
DR EnsemblBacteria; AAL22888; AAL22888; STM4048.
DR GeneID; 1255575; -.
DR KEGG; stm:STM4048; -.
DR PATRIC; fig|99287.12.peg.4265; -.
DR HOGENOM; CLU_000445_88_5_6; -.
DR OMA; GHYPSHW; -.
DR PhylomeDB; P0A2S9; -.
DR BioCyc; SENT99287:STM4048-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR GO; GO:0019299; P:rhamnose metabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 2.60.120.10; -; 1.
DR HAMAP; MF_01534; HTH_type_RhaS; 1.
DR InterPro; IPR003313; AraC-bd.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR037923; HTH-like.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR InterPro; IPR023609; Tscrpt_reg_HTH_RhaS.
DR Pfam; PF02311; AraC_binding; 1.
DR Pfam; PF12833; HTH_18; 1.
DR PRINTS; PR00032; HTHARAC.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; SSF46689; 2.
DR SUPFAM; SSF51215; SSF51215; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 3: Inferred from homology;
KW Activator; Cytoplasm; DNA-binding; Reference proteome; Repeat;
KW Rhamnose metabolism; Transcription; Transcription regulation.
FT CHAIN 1..278
FT /note="HTH-type transcriptional activator RhaS"
FT /id="PRO_0000194571"
FT DOMAIN 174..272
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT DNA_BIND 191..212
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT DNA_BIND 239..262
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT SITE 241
FT /note="Interaction with sigma-70"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT SITE 250
FT /note="Interaction with sigma-70"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT CONFLICT 100..104
FT /note="QLLPQ -> HCCR (in Ref. 1; CAA40556)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 278 AA; 32089 MW; A8451BF20C1F4297 CRC64;
MTVLHSVDFF PSGKAPVAIE PRLPQAAFPE HHHDFHEIVI VEHGTGIHVF NGQPYTISGG
TVCFVRDHDR HLYEHTDNLC LTNVLWRSPD AFQFLAGLDQ LLPQEQDGYY PSHWRVNQSV
LQQVRQLVGL MERAGDGMDA PAVANREILF MQLLVLLRRS SLMEGATNND AKLNQLMAWL
EDHFAEEVCW EAVAEQFSLS LRTLHRQLKQ HTGLTPQRYL NRLRLIKARH LLRHSDHSVT
EIAYRCGFGD SNHFSTLFRR EFNWSPRDIR QGRDAIIQ
