RHAS_SHIFL
ID RHAS_SHIFL Reviewed; 278 AA.
AC Q83PE0; Q7BZF3;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=HTH-type transcriptional activator RhaS {ECO:0000255|HAMAP-Rule:MF_01534};
DE AltName: Full=L-rhamnose operon regulatory protein RhaS {ECO:0000255|HAMAP-Rule:MF_01534};
GN Name=rhaS {ECO:0000255|HAMAP-Rule:MF_01534};
GN OrderedLocusNames=SF3982, S3766;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: Activates expression of the rhaBAD and rhaT operons.
CC {ECO:0000255|HAMAP-Rule:MF_01534}.
CC -!- SUBUNIT: Binds DNA as a dimer. {ECO:0000255|HAMAP-Rule:MF_01534}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01534}.
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DR EMBL; AE005674; AAN45416.1; -; Genomic_DNA.
DR EMBL; AE014073; AAP18783.1; -; Genomic_DNA.
DR RefSeq; NP_709709.1; NC_004337.2.
DR RefSeq; WP_000217105.1; NZ_WPGW01000095.1.
DR AlphaFoldDB; Q83PE0; -.
DR SMR; Q83PE0; -.
DR STRING; 198214.SF3982; -.
DR EnsemblBacteria; AAN45416; AAN45416; SF3982.
DR EnsemblBacteria; AAP18783; AAP18783; S3766.
DR GeneID; 1027382; -.
DR GeneID; 58391211; -.
DR KEGG; sfl:SF3982; -.
DR KEGG; sfx:S3766; -.
DR PATRIC; fig|198214.7.peg.4692; -.
DR HOGENOM; CLU_000445_88_5_6; -.
DR OMA; GHYPSHW; -.
DR OrthoDB; 1268974at2; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR GO; GO:0019299; P:rhamnose metabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 2.60.120.10; -; 1.
DR HAMAP; MF_01534; HTH_type_RhaS; 1.
DR InterPro; IPR003313; AraC-bd.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR037923; HTH-like.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR InterPro; IPR023609; Tscrpt_reg_HTH_RhaS.
DR Pfam; PF02311; AraC_binding; 1.
DR Pfam; PF12833; HTH_18; 1.
DR PRINTS; PR00032; HTHARAC.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; SSF46689; 2.
DR SUPFAM; SSF51215; SSF51215; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 3: Inferred from homology;
KW Activator; Cytoplasm; DNA-binding; Reference proteome; Repeat;
KW Rhamnose metabolism; Transcription; Transcription regulation.
FT CHAIN 1..278
FT /note="HTH-type transcriptional activator RhaS"
FT /id="PRO_0000194572"
FT DOMAIN 174..272
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT DNA_BIND 191..212
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT DNA_BIND 239..262
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT SITE 241
FT /note="Interaction with sigma-70"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
FT SITE 250
FT /note="Interaction with sigma-70"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01534"
SQ SEQUENCE 278 AA; 32361 MW; 64271150484E74EA CRC64;
MTVLHSVDFF PSCNASVAIE PRLPQADFPE HHHDFHEIVI VEHGTGIHVF NGQPYTITGG
TVCFVRDHDR HLYEHTDNLC LTNVLYRSPD RFQFLAGLNQ LLPQELDGQY PSHWRVNHSV
LQQVRQLVAQ MEQQEGENDL PSTASREILF MQLLLLLRKS SLQENLENSA SRLNLLLAWL
EDHFADEVNW DAVADQFSLS LRTLHRQLKQ QTGLTPQRYL NRLRLMKARH LLRHSEASVT
DIAYRCGFSD SNHFSTLFRR EFNWSPRDIR QGRDGFLQ
