RHB1_SCHPO
ID RHB1_SCHPO Reviewed; 185 AA.
AC O94363;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=GTP-binding protein rhb1;
DE AltName: Full=GTP-binding protein Rheb homolog;
DE Flags: Precursor;
GN Name=rhb1; ORFNames=SPBC428.16c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION.
RX PubMed=10835385; DOI=10.1093/genetics/155.2.611;
RA Mach K.E., Furge K.A., Albright C.F.;
RT "Loss of Rhb1, a Rheb-related GTPase in fission yeast, causes growth arrest
RT with a terminal phenotype similar to that caused by nitrogen starvation.";
RL Genetics 155:611-622(2000).
CC -!- FUNCTION: Regulates entry into stationary phase when extracellular
CC nitrogen levels are adequate for growth. {ECO:0000269|PubMed:10835385}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}; Cytoplasmic side {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rheb family.
CC {ECO:0000305}.
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DR EMBL; CU329671; CAA22291.1; -; Genomic_DNA.
DR PIR; T40468; T40468.
DR RefSeq; NP_595194.1; NM_001021101.2.
DR AlphaFoldDB; O94363; -.
DR SMR; O94363; -.
DR BioGRID; 277370; 17.
DR STRING; 4896.SPBC428.16c.1; -.
DR iPTMnet; O94363; -.
DR MaxQB; O94363; -.
DR PaxDb; O94363; -.
DR PRIDE; O94363; -.
DR EnsemblFungi; SPBC428.16c.1; SPBC428.16c.1:pep; SPBC428.16c.
DR GeneID; 2540853; -.
DR KEGG; spo:SPBC428.16c; -.
DR PomBase; SPBC428.16c; rhb1.
DR VEuPathDB; FungiDB:SPBC428.16c; -.
DR eggNOG; KOG0395; Eukaryota.
DR HOGENOM; CLU_041217_9_8_1; -.
DR InParanoid; O94363; -.
DR OMA; SARHNEN; -.
DR PhylomeDB; O94363; -.
DR Reactome; R-SPO-165159; MTOR signalling.
DR Reactome; R-SPO-9639288; Amino acids regulate mTORC1.
DR PRO; PR:O94363; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0031234; C:extrinsic component of cytoplasmic side of plasma membrane; ISO:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0019003; F:GDP binding; IDA:PomBase.
DR GO; GO:0005525; F:GTP binding; IDA:UniProtKB.
DR GO; GO:0003924; F:GTPase activity; IDA:UniProtKB.
DR GO; GO:0043539; F:protein serine/threonine kinase activator activity; IGI:PomBase.
DR GO; GO:1905589; P:positive regulation of L-arginine import across plasma membrane; IGI:PomBase.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IBA:GO_Central.
DR GO; GO:0038202; P:TORC1 signaling; IGI:PomBase.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR020849; Small_GTPase_Ras-type.
DR PANTHER; PTHR24070; PTHR24070; 1.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51421; RAS; 1.
PE 3: Inferred from homology;
KW Cell membrane; GTP-binding; Lipoprotein; Membrane; Methylation;
KW Nucleotide-binding; Prenylation; Reference proteome.
FT CHAIN 1..182
FT /note="GTP-binding protein rhb1"
FT /id="PRO_0000082712"
FT PROPEP 183..185
FT /note="Removed in mature form"
FT /evidence="ECO:0000250"
FT /id="PRO_0000281369"
FT MOTIF 35..43
FT /note="Effector region"
FT BINDING 13..20
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 60..64
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 119..122
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT MOD_RES 182
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250"
FT LIPID 182
FT /note="S-farnesyl cysteine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 185 AA; 20515 MW; 3DBC3E352C122C71 CRC64;
MAPIKSRRIA VLGSRSVGKS SLTVQYVENH FVESYYPTIE NTFSKNIKYK GQEFATEIID
TAGQDEYSIL NSKHSIGIHG YVLVYSITSK SSFEMVKIVR DKILNHTGTE WVPIVVVGNK
SDLHMQRAVT AEEGKALANE WKCAWTEASA RHNENVARAF ELIISEIEKQ ANPSPPGDGK
GCVIA
