RHBG_BOVIN
ID RHBG_BOVIN Reviewed; 455 AA.
AC Q95M77; A3KMW3;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Ammonium transporter Rh type B;
DE AltName: Full=Rhesus blood group family type B glycoprotein;
DE Short=Rh family type B glycoprotein;
DE Short=Rh type B glycoprotein;
GN Name=RHBG;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=11358367; DOI=10.1006/bcmd.2000.0355;
RA Huang C.-H., Liu P.Z.;
RT "New insights into the Rh superfamily of genes and proteins in erythroid
RT cells and nonerythroid tissues.";
RL Blood Cells Mol. Dis. 27:90-101(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Hereford; TISSUE=Fetal skin;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Functions as a specific ammonium transporter. {ECO:0000250}.
CC -!- SUBUNIT: Interacts (via C-terminus) with ANK2 and ANK3; required for
CC targeting to the basolateral membrane. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Basolateral cell membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}. Cytoplasmic vesicle membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q95M77-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q95M77-2; Sequence=VSP_036401, VSP_036402;
CC -!- PTM: N-glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ammonium transporter (TC 2.A.49) family. Rh
CC subfamily. {ECO:0000305}.
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DR EMBL; AY013259; AAK14649.1; -; mRNA.
DR EMBL; BC133318; AAI33319.1; -; mRNA.
DR RefSeq; NP_777148.1; NM_174723.2.
DR RefSeq; XP_005203673.1; XM_005203616.3. [Q95M77-1]
DR AlphaFoldDB; Q95M77; -.
DR SMR; Q95M77; -.
DR STRING; 9913.ENSBTAP00000046932; -.
DR PaxDb; Q95M77; -.
DR Ensembl; ENSBTAT00000050191; ENSBTAP00000046932; ENSBTAG00000012234. [Q95M77-1]
DR GeneID; 282709; -.
DR KEGG; bta:282709; -.
DR CTD; 57127; -.
DR VEuPathDB; HostDB:ENSBTAG00000012234; -.
DR eggNOG; KOG3796; Eukaryota.
DR GeneTree; ENSGT00950000182844; -.
DR HOGENOM; CLU_021386_0_0_1; -.
DR InParanoid; Q95M77; -.
DR OMA; IFVRYNH; -.
DR OrthoDB; 910733at2759; -.
DR TreeFam; TF314450; -.
DR Reactome; R-BTA-444411; Rhesus glycoproteins mediate ammonium transport.
DR Proteomes; UP000009136; Chromosome 3.
DR Bgee; ENSBTAG00000012234; Expressed in urethra and 85 other tissues.
DR ExpressionAtlas; Q95M77; baseline and differential.
DR GO; GO:0046658; C:anchored component of plasma membrane; IEA:Ensembl.
DR GO; GO:0016323; C:basolateral plasma membrane; ISS:UniProtKB.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0014731; C:spectrin-associated cytoskeleton; ISS:UniProtKB.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0030506; F:ankyrin binding; IEA:Ensembl.
DR GO; GO:0097272; P:ammonium homeostasis; IBA:GO_Central.
DR GO; GO:0072488; P:ammonium transmembrane transport; ISS:UniProtKB.
DR GO; GO:0070634; P:transepithelial ammonium transport; IEA:Ensembl.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR InterPro; IPR002229; RhesusRHD.
DR Pfam; PF00909; Ammonium_transp; 1.
DR PRINTS; PR00342; RHESUSRHD.
PE 2: Evidence at transcript level;
KW Alternative splicing; Ammonia transport; Cell membrane;
KW Cytoplasmic vesicle; Glycoprotein; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..455
FT /note="Ammonium transporter Rh type B"
FT /id="PRO_0000283594"
FT TOPO_DOM 1..13
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..61
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..86
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..124
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 146..151
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 173..179
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..219
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 241..253
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 275..277
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 278..298
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 299
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 321..343
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 344..364
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 365..393
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 394..414
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 415..455
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 416..424
FT /note="Interaction with ANK3"
FT /evidence="ECO:0000250"
FT MOTIF 429..432
FT /note="Basolateral sorting signal"
FT /evidence="ECO:0000250"
FT CARBOHYD 49
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 168
FT /note="F -> FVLLSLLEVVLFGLNEF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11358367"
FT /id="VSP_036401"
FT VAR_SEQ 281..326
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11358367"
FT /id="VSP_036402"
FT CONFLICT 208..209
FT /note="Missing (in Ref. 2; AAI33319)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 455 AA; 49193 MW; 4BAB3BBE2D26E9EE CRC64;
MAWSPRHSAG RRLQLPLLCL LLQGATAILF AVFVRYNRET DAALWHWGNH SNADNEFYFR
YPSFQDVHAM IFVGFGFLMV FLQRYGFGSV GFTFLLAAFA LQWSTLIQGF FHSFRGGYIL
VGMESMINAD FCAGAVLISF GAVLGKTGPV QLLLMALLEV VLFGLNEFVL LSLLEVKDAG
GSMTIHTFGA YFGLILSRVL YRPQLEKSKH RQGSVYHSDL FAMIGTIFLW IFWPSFNSAP
TALGDGQHRT ALNTYYSLTA STLSTFALSA LVGGDGRLDM VHVQNAALAG GVVVGTSAEM
MLTPFGALAA GFLAGAISTL GYKFVTPILE SKLKVQDTCG VHNLHGMPGV LGALLGGLVA
GLATREAYGD GLESVFPLIA EGQRSATSQA MHQLFGLFVT LTFASVGGGL GGLLLRLPIL
DSPPDSQCYE DQIYWEVPGE HEHLAQGSEE TETQA
