RHBG_CANLF
ID RHBG_CANLF Reviewed; 458 AA.
AC Q4VUI0; Q8HYQ5; Q8HYQ6;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Ammonium transporter Rh type B;
DE AltName: Full=Rhesus blood group family type B glycoprotein;
DE Short=Rh family type B glycoprotein;
DE Short=Rh type B glycoprotein;
GN Name=RHBG;
OS Canis lupus familiaris (Dog) (Canis familiaris).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX NCBI_TaxID=9615;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Kidney;
RX PubMed=16227429; DOI=10.1073/pnas.0507886102;
RA Huang C.-H., Peng J.;
RT "Evolutionary conservation and diversification of Rh family genes and
RT proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:15512-15517(2005).
CC -!- FUNCTION: Functions as a specific ammonium transporter. {ECO:0000250}.
CC -!- SUBUNIT: Interacts (via C-terminus) with ANK2 and ANK3; required for
CC targeting to the basolateral membrane. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Basolateral cell membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}. Cytoplasmic vesicle membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q4VUI0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q4VUI0-2; Sequence=VSP_024339;
CC -!- PTM: N-glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ammonium transporter (TC 2.A.49) family. Rh
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN76726.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AY622224; AAV40851.1; -; mRNA.
DR EMBL; AF447922; AAN76725.1; -; mRNA.
DR EMBL; AF447923; AAN76726.1; ALT_INIT; mRNA.
DR RefSeq; NP_001003017.2; NM_001003017.2. [Q4VUI0-1]
DR AlphaFoldDB; Q4VUI0; -.
DR SMR; Q4VUI0; -.
DR STRING; 9612.ENSCAFP00000033031; -.
DR Ensembl; ENSCAFT00030002003; ENSCAFP00030001775; ENSCAFG00030001157. [Q4VUI0-2]
DR Ensembl; ENSCAFT00030002016; ENSCAFP00030001785; ENSCAFG00030001157. [Q4VUI0-1]
DR Ensembl; ENSCAFT00040037227; ENSCAFP00040032431; ENSCAFG00040020141. [Q4VUI0-2]
DR Ensembl; ENSCAFT00845016469; ENSCAFP00845012807; ENSCAFG00845009336. [Q4VUI0-2]
DR Ensembl; ENSCAFT00845016511; ENSCAFP00845012842; ENSCAFG00845009336. [Q4VUI0-1]
DR GeneID; 403538; -.
DR KEGG; cfa:403538; -.
DR CTD; 57127; -.
DR VEuPathDB; HostDB:ENSCAFG00845009336; -.
DR eggNOG; KOG3796; Eukaryota.
DR GeneTree; ENSGT00950000182844; -.
DR HOGENOM; CLU_021386_0_0_1; -.
DR InParanoid; Q4VUI0; -.
DR OMA; IFVRYNH; -.
DR OrthoDB; 910733at2759; -.
DR Reactome; R-CFA-444411; Rhesus glycoproteins mediate ammonium transport.
DR Proteomes; UP000002254; Chromosome 7.
DR Bgee; ENSCAFG00000016811; Expressed in testis and 46 other tissues.
DR GO; GO:0046658; C:anchored component of plasma membrane; IEA:Ensembl.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:UniProtKB.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0014731; C:spectrin-associated cytoskeleton; ISS:UniProtKB.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0030506; F:ankyrin binding; IEA:Ensembl.
DR GO; GO:0097272; P:ammonium homeostasis; IBA:GO_Central.
DR GO; GO:0072488; P:ammonium transmembrane transport; IDA:UniProtKB.
DR GO; GO:0070634; P:transepithelial ammonium transport; IEA:Ensembl.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR InterPro; IPR002229; RhesusRHD.
DR Pfam; PF00909; Ammonium_transp; 1.
DR PRINTS; PR00342; RHESUSRHD.
PE 2: Evidence at transcript level;
KW Alternative splicing; Ammonia transport; Cell membrane;
KW Cytoplasmic vesicle; Glycoprotein; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..458
FT /note="Ammonium transporter Rh type B"
FT /id="PRO_0000283595"
FT TOPO_DOM 1..13
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..61
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..90
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..124
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 146..151
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 173..179
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..219
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 241..250
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 274..279
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 303..306
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 307..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 330..343
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 344..364
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 365..393
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 394..414
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 415..458
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 416..424
FT /note="Interaction with ANK3"
FT /evidence="ECO:0000250"
FT MOTIF 429..432
FT /note="Basolateral sorting signal"
FT /evidence="ECO:0000250"
FT CARBOHYD 49
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..63
FT /note="MARSPRRAGAPRLQLPLLCLLLQGATAILFAVFVRYNHETDAALWHWGNHSN
FT LDNEFYFRYPS -> MEQHASHPNEKAKEESNAVPLCRAKPSPSFINLQASSPPATFLN
FT IQRTKLPSG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16227429"
FT /id="VSP_024339"
SQ SEQUENCE 458 AA; 49523 MW; F966A0DDE22774CA CRC64;
MARSPRRAGA PRLQLPLLCL LLQGATAILF AVFVRYNHET DAALWHWGNH SNLDNEFYFR
YPSFQDVHVM VFVGFGFLMA FLQRYGFSSV GFTFLLAAFA LQWSTLIQGF FHSLHGGYIH
VSVNSMINAD FCAGAVLISF GAILGKTGPA QLLLMTVLEV ALFGINEFVL LNLLQVKDAG
GSMTIHTFGA YFGLVLSRVL YRPQLEKSKH RQCSVYHSDL FAMIGTIFLW IFWPSFNSAP
TTLGDGQHRT ALNTYYSLSA STLGTFAMSA LVGERGRLDM VHIQNAALAG GVVVGTAGEM
MLTPFGALAA GFLAGTVSTL GYKFFTPILE AKFKVQDTCG VHNLHGIPGV LGALLGVLVV
GLATHEAYGE GLGSVFPLIA KGQRTAMSQA MYQLFGLFVT LMFASVGGAL GGLLLKLPCL
GSPADCQCYE DQVYWEVPGE HEDAAQGPLK AEEPDTQA
