RHBL3_MOUSE
ID RHBL3_MOUSE Reviewed; 404 AA.
AC P58873;
DT 20-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Rhomboid-related protein 3;
DE EC=3.4.21.105;
DE AltName: Full=Ventrhoid transmembrane protein;
GN Name=Rhbdl3; Synonyms=Rhbdl4, Vrho;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=11900977; DOI=10.1016/s0925-4773(01)00655-4;
RA Jaszai J., Brand M.;
RT "Cloning and expression of Ventrhoid, a novel vertebrate homologue of the
RT Drosophila EGF pathway gene Rhomboid.";
RL Mech. Dev. 113:73-77(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May be involved in regulated intramembrane proteolysis and
CC the subsequent release of functional polypeptides from their membrane
CC anchors. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Cleaves type-1 transmembrane domains using a catalytic dyad
CC composed of serine and histidine that are contributed by different
CC transmembrane domains.; EC=3.4.21.105;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: At 8 dpc, expression is limited to the developing
CC central nervous system (CNS). From 9 dpc on detected in the ventral
CC forebrain, pretectum, dorsal diencephalon, metencephalon, the ventral
CC spinal neural tube, in the ectoderm of the developing mandibular arches
CC and the developing hindgut.
CC -!- SIMILARITY: Belongs to the peptidase S54 family. {ECO:0000305}.
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DR EMBL; AJ313479; CAC86144.1; -; mRNA.
DR EMBL; BC054784; AAH54784.1; -; mRNA.
DR EMBL; BC056969; AAH56969.1; -; mRNA.
DR CCDS; CCDS25131.1; -.
DR RefSeq; NP_631974.1; NM_139228.3.
DR AlphaFoldDB; P58873; -.
DR SMR; P58873; -.
DR STRING; 10090.ENSMUSP00000017836; -.
DR BindingDB; P58873; -.
DR MEROPS; S54.006; -.
DR iPTMnet; P58873; -.
DR PhosphoSitePlus; P58873; -.
DR PaxDb; P58873; -.
DR PRIDE; P58873; -.
DR ProteomicsDB; 253269; -.
DR Antibodypedia; 71802; 6 antibodies from 6 providers.
DR DNASU; 246104; -.
DR Ensembl; ENSMUST00000017836; ENSMUSP00000017836; ENSMUSG00000017692.
DR GeneID; 246104; -.
DR KEGG; mmu:246104; -.
DR UCSC; uc007klt.2; mouse.
DR CTD; 162494; -.
DR MGI; MGI:2179276; Rhbdl3.
DR VEuPathDB; HostDB:ENSMUSG00000017692; -.
DR eggNOG; KOG0027; Eukaryota.
DR eggNOG; KOG2289; Eukaryota.
DR GeneTree; ENSGT00940000158838; -.
DR HOGENOM; CLU_048023_2_1_1; -.
DR InParanoid; P58873; -.
DR OMA; KFDPGST; -.
DR OrthoDB; 1253228at2759; -.
DR PhylomeDB; P58873; -.
DR TreeFam; TF313540; -.
DR BioGRID-ORCS; 246104; 0 hits in 73 CRISPR screens.
DR ChiTaRS; Rhbdl3; mouse.
DR PRO; PR:P58873; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; P58873; protein.
DR Bgee; ENSMUSG00000017692; Expressed in ventricular zone and 191 other tissues.
DR Genevisible; P58873; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1540.10; -; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR022764; Peptidase_S54_rhomboid_dom.
DR InterPro; IPR017213; Peptidase_S54_rhomboid_met.
DR InterPro; IPR035952; Rhomboid-like_sf.
DR Pfam; PF01694; Rhomboid; 1.
DR PIRSF; PIRSF037470; Rhomboid; 1.
DR SUPFAM; SSF144091; SSF144091; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 2: Evidence at transcript level;
KW Hydrolase; Membrane; Protease; Reference proteome; Repeat; Serine protease;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..404
FT /note="Rhomboid-related protein 3"
FT /id="PRO_0000206178"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..358
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 371..391
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 34..69
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 70..105
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT ACT_SITE 278
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 343
FT /evidence="ECO:0000250"
SQ SEQUENCE 404 AA; 45266 MW; D738F903C45AC524 CRC64;
MGEHPSPGPA VAACAEAERI EELEPEAEER LPAAPEDHWK VLFEKFDPGS TGYISTGKFR
SLLESHSSKL DPHKKEVLLA LADSHADGQI CYQDFVNLMS NKRSNSFRQA ILQGNRRLSS
KALLEEKGLS LSQRLIRHVA YETLPREIDR KWYYDSYTCC PPPWFMITIT LLEVALFLYN
GVLLDQFVLQ VTHPRYLKNS LVYHPQLRAQ AWRYVTYIFM HAGVEQLGLN VALQLLVGVP
LEMVHGATRI GLVYVAGVVA GSLAVSVADM TAPVVGSSGG VYALVSAHLA NIVMNWSGMK
CQFKLLRMAV ALICMSMEFG RAVWLRFHPS AYPPCPHPSF VAHLGGVAVG ITLGVVVLRN
YEQRLQDQSL WWIFVTMYTI FVLFAVFWNI FAYTLLDLKL PPAP
