ID   RHBL6_TOXGO             Reviewed;         531 AA.
AC   Q2PP52;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=Rhomboid-like protease 6;
DE            EC=3.4.21.105;
GN   Name=ROM6;
OS   Toxoplasma gondii.
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC   Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma.
OX   NCBI_TaxID=5811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=RH;
RA   Dowse T.J., Soldati D.;
RT   "Rhomboid-like protease 6 from Toxoplasma gondii.";
RL   Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Putative serine protease involved in intramembrane
CC       proteolysis and the subsequent release of polypeptides from their
CC       membrane anchors. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Cleaves type-1 transmembrane domains using a catalytic dyad
CC         composed of serine and histidine that are contributed by different
CC         transmembrane domains.; EC=3.4.21.105;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase S54 family. {ECO:0000305}.
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DR   EMBL; DQ314573; ABC40721.1; -; mRNA.
DR   AlphaFoldDB; Q2PP52; -.
DR   VEuPathDB; ToxoDB:TGARI_245590A; -.
DR   VEuPathDB; ToxoDB:TGCAST_245590A; -.
DR   VEuPathDB; ToxoDB:TGCOUG_245590; -.
DR   VEuPathDB; ToxoDB:TGDOM2_245590; -.
DR   VEuPathDB; ToxoDB:TGFOU_245590; -.
DR   VEuPathDB; ToxoDB:TGGT1_245590A; -.
DR   VEuPathDB; ToxoDB:TGMAS_245590; -.
DR   VEuPathDB; ToxoDB:TGME49_245590; -.
DR   VEuPathDB; ToxoDB:TGP89_245590A; -.
DR   VEuPathDB; ToxoDB:TGPRC2_245590; -.
DR   VEuPathDB; ToxoDB:TGRH88_060210; -.
DR   VEuPathDB; ToxoDB:TGRUB_245590; -.
DR   VEuPathDB; ToxoDB:TGVAND_245590A; -.
DR   VEuPathDB; ToxoDB:TGVEG_245590; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1540.10; -; 1.
DR   InterPro; IPR022764; Peptidase_S54_rhomboid_dom.
DR   InterPro; IPR035952; Rhomboid-like_sf.
DR   Pfam; PF01694; Rhomboid; 1.
DR   SUPFAM; SSF144091; SSF144091; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Membrane; Protease; Serine protease; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..531
FT                   /note="Rhomboid-like protease 6"
FT                   /id="PRO_0000239079"
FT   TRANSMEM        277..297
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        307..327
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        367..387
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        407..427
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        440..460
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        484..504
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          150..190
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        152..175
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        419
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        493
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   531 AA;  58688 MW;  D94F4FA78169D990 CRC64;
     MKKSAGPFIA SWRPFVGLPV SLERGRTFSR VLGICTYTPA IRRQMLLSGV SVIPPPLSKN
     FFRHAHLTGF SPFRSSCRRL FAQNGSDVAP LVSRIPNQSV APGDTQWSVS PMGVHCEHWL
     KTRCLPGLPA AVFGSHQQVE AALLQRGLSP AKDASERVRS ATREDLVRRE RPTSEAGSHP
     PLPSRSSVSA FQLRSRCDPA VSGPSDSAPC GLSQTSFSLR SSSSSGATTR FSVPAVCPVF
     LCRSLPRLSG RFSRTRFGKQ RKRTEEQEDS KDFDTGPLSS GSIFTVLVGS LLFFDVLRSG
     LYEHRKLLGA FLMTNAAVFT GWRLAATAGN GTWWTFLMRH FVLCRENLAR ARIYTFVTSS
     LSHKSTGHLI FNLFVINQLF RLLSFDLSDR DFASLFGLAA LCGGLGHLLV SRQPVLGASA
     FAYALLWTEA TRHSREMFRI LPIPFFPLTA LQLVQVAMCL EAAMAFLARP AFLARFPASR
     LLRFAQGISW SGHLGGLAAG CLYSWYKRRV EGDRSWQSFS ELCRTYGTSD W