RHCG_RAT
ID RHCG_RAT Reviewed; 497 AA.
AC Q7TNN9;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Ammonium transporter Rh type C;
DE AltName: Full=Rhesus blood group family type C glycoprotein;
DE Short=Rh family type C glycoprotein;
DE Short=Rh type C glycoprotein;
GN Name=Rhcg;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=16227429; DOI=10.1073/pnas.0507886102;
RA Huang C.-H., Peng J.;
RT "Evolutionary conservation and diversification of Rh family genes and
RT proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:15512-15517(2005).
RN [2]
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=12138130; DOI=10.1097/01.asn.0000025280.02386.9d;
RA Eladari D., Cheval L., Quentin F., Bertrand O., Mouro I., Cherif-Zahar B.,
RA Cartron J.-P., Paillard M., Doucet A., Chambrey R.;
RT "Expression of RhCG, a new putative NH(3)/NH(4)(+) transporter, along the
RT rat nephron.";
RL J. Am. Soc. Nephrol. 13:1999-2008(2002).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=12595489; DOI=10.1097/01.asn.0000050413.43662.55;
RA Quentin F., Eladari D., Cheval L., Lopez C., Goossens D., Colin Y.,
RA Cartron J.-P., Paillard M., Chambrey R.;
RT "RhBG and RhCG, the putative ammonia transporters, are expressed in the
RT same cells in the distal nephron.";
RL J. Am. Soc. Nephrol. 14:545-554(2003).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=16434569; DOI=10.1152/ajprenal.00459.2005;
RA Seshadri R.M., Klein J.D., Smith T., Sands J.M., Handlogten M.E.,
RA Verlander J.W., Weiner I.D.;
RT "Changes in subcellular distribution of the ammonia transporter, Rhcg, in
RT response to chronic metabolic acidosis.";
RL Am. J. Physiol. 290:F1443-F1452(2006).
CC -!- FUNCTION: Functions as an electroneutral and bidirectional ammonium
CC transporter. May regulate transepithelial ammonia secretion (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homotrimer.
CC -!- SUBCELLULAR LOCATION: Apical cell membrane
CC {ECO:0000269|PubMed:12138130, ECO:0000269|PubMed:12595489,
CC ECO:0000269|PubMed:16434569}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:12138130, ECO:0000269|PubMed:12595489,
CC ECO:0000269|PubMed:16434569}. Note=Also detected at the basolateral
CC membrane and in subapical vesicles. Chronic acidosis induces
CC relocalization to the apical cell membrane.
CC -!- TISSUE SPECIFICITY: Expressed by connecting tubule cells and
CC intercalated cells of the collecting duct in kidney (at protein level).
CC {ECO:0000269|PubMed:12138130}.
CC -!- PTM: N-glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ammonium transporter (TC 2.A.49) family. Rh
CC subfamily. {ECO:0000305}.
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DR EMBL; AY129073; AAN07791.1; -; mRNA.
DR RefSeq; NP_898876.1; NM_183053.1.
DR AlphaFoldDB; Q7TNN9; -.
DR SMR; Q7TNN9; -.
DR STRING; 10116.ENSRNOP00000021109; -.
DR GlyGen; Q7TNN9; 1 site.
DR PaxDb; Q7TNN9; -.
DR GeneID; 293048; -.
DR KEGG; rno:293048; -.
DR UCSC; RGD:727859; rat.
DR CTD; 51458; -.
DR RGD; 727859; Rhcg.
DR eggNOG; KOG3796; Eukaryota.
DR InParanoid; Q7TNN9; -.
DR OrthoDB; 910733at2759; -.
DR PhylomeDB; Q7TNN9; -.
DR Reactome; R-RNO-444411; Rhesus glycoproteins mediate ammonium transport.
DR PRO; PR:Q7TNN9; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0016324; C:apical plasma membrane; IDA:UniProtKB.
DR GO; GO:0009925; C:basal plasma membrane; IDA:RGD.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:UniProtKB.
DR GO; GO:0031410; C:cytoplasmic vesicle; IDA:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0030506; F:ankyrin binding; ISS:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR GO; GO:0097272; P:ammonium homeostasis; IBA:GO_Central.
DR GO; GO:0072488; P:ammonium transmembrane transport; IDA:UniProtKB.
DR GO; GO:0006873; P:cellular ion homeostasis; ISS:UniProtKB.
DR GO; GO:0006885; P:regulation of pH; ISO:RGD.
DR GO; GO:0070634; P:transepithelial ammonium transport; ISS:UniProtKB.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR InterPro; IPR002229; RhesusRHD.
DR Pfam; PF00909; Ammonium_transp; 1.
DR PRINTS; PR00342; RHESUSRHD.
PE 1: Evidence at protein level;
KW Ammonia transport; Cell membrane; Glycoprotein; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..497
FT /note="Ammonium transporter Rh type C"
FT /id="PRO_0000283584"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..61
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..93
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 115..125
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 146..151
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 175..179
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..219
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 241..251
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 273..282
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 304
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 326..340
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..361
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 362..395
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 396..416
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 417..497
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 48
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 497 AA; 54731 MW; C696D975833F3B67 CRC64;
MAWNTNLRGR LPITCLILQV TMVVLFGVFV RYDIQADAHW WLEKKRKNIS SDVENEFYYR
YPSFEDVHAM VFVGFGFLMT YLQRYGFSAV GFNFLLAAFG IQWALLMQGW FHFFEEGHIL
LSVENLIQAD FCVASTCVAF GAVLGKISPM QLLIMTFFQV TLFTVNEFIL LNLIEAKDAG
GSMTIHTFGA YFGLTVTWIL YRKNLEQSKQ RQSSVYHSDL FAMIGTLFLW IYWPSFNSAS
SFHGDTQHRA ALNTYLSLAA SVLTTVAVSS VIHKKGKLDM VHIQNATLAG GVGVGTAAEM
MLTPYGALIV GFFCGILSTL GFAYLSPFLE SRLRIQDTCG IHNLHGIPGI IGGIVGAVTA
AYSSPDVYGE PGIVHSFGFG GYKADWTKRM QGRSQIFGLL LSLAMALVGG IIVGFILKLP
FWGQASDENC FEDAIYWEVP EEVNTVYIPE DLAHKHSTSL VPAIPLVLST PSASIVPPVP
PTPPASLATV TSSSLVH
