RHCL2_DANRE
ID RHCL2_DANRE Reviewed; 488 AA.
AC Q8JI14; A5LHA7; Q6NYR3;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Ammonium transporter Rh type C-like 2;
DE AltName: Full=Rhesus blood group family type C glycoprotein-like 2;
DE Short=Rh family type C glycoprotein-like 2;
DE Short=Rh type C glycoprotein-like 2;
DE AltName: Full=drRhcg1;
GN Name=rhcgl2; Synonyms=rhcg, rhcg1; ORFNames=zgc:77520;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=16227429; DOI=10.1073/pnas.0507886102;
RA Huang C.-H., Peng J.;
RT "Evolutionary conservation and diversification of Rh family genes and
RT proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:15512-15517(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=17686885; DOI=10.1152/ajpregu.00248.2007;
RA Nakada T., Hoshijima K., Esaki M., Nagayoshi S., Kawakami K., Hirose S.;
RT "Localization of ammonia transporter Rhcg1 in mitochondrion-rich cells of
RT yolk sac, gill, and kidney of zebrafish and its ionic strength-dependent
RT expression.";
RL Am. J. Physiol. 293:R1743-R1753(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Functions as an ammonia transporter. May play a role in the
CC elimination of ammonia in the gill (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homotrimer.
CC -!- SUBCELLULAR LOCATION: Apical cell membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: At larval stages, expressed only in the yolk sac
CC and gill. However, the kidney and the gills are major sites of
CC expression in adults. {ECO:0000269|PubMed:17686885}.
CC -!- SIMILARITY: Belongs to the ammonium transporter (TC 2.A.49) family. Rh
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH66492.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF398238; AAM90586.1; -; mRNA.
DR EMBL; AB286865; BAF63790.1; -; mRNA.
DR EMBL; BC066492; AAH66492.1; ALT_FRAME; mRNA.
DR AlphaFoldDB; Q8JI14; -.
DR SMR; Q8JI14; -.
DR IntAct; Q8JI14; 1.
DR STRING; 7955.ENSDARP00000053517; -.
DR PaxDb; Q8JI14; -.
DR ZFIN; ZDB-GENE-040426-2595; rhcgb.
DR eggNOG; KOG3796; Eukaryota.
DR InParanoid; Q8JI14; -.
DR PhylomeDB; Q8JI14; -.
DR PRO; PR:Q8JI14; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0016324; C:apical plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; ISS:ZFIN.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0097272; P:ammonium homeostasis; IMP:ZFIN.
DR GO; GO:0072488; P:ammonium transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR InterPro; IPR002229; RhesusRHD.
DR Pfam; PF00909; Ammonium_transp; 1.
DR PRINTS; PR00342; RHESUSRHD.
PE 2: Evidence at transcript level;
KW Ammonia transport; Cell membrane; Glycoprotein; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..488
FT /note="Ammonium transporter Rh type C-like 2"
FT /id="PRO_0000283586"
FT TOPO_DOM 1..21
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 43..73
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 95..98
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..139
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 161..162
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..191
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 192..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 215..232
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 254..264
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 286..298
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..319
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 320
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 342..356
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 378..409
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 410..430
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 431..488
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 60
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 29
FT /note="V -> K (in Ref. 1; AAM90586)"
FT /evidence="ECO:0000305"
FT CONFLICT 51
FT /note="N -> H (in Ref. 1; AAM90586)"
FT /evidence="ECO:0000305"
FT CONFLICT 58
FT /note="E -> D (in Ref. 1; AAM90586)"
FT /evidence="ECO:0000305"
FT CONFLICT 61
FT /note="I -> K (in Ref. 1; AAM90586)"
FT /evidence="ECO:0000305"
FT CONFLICT 124..125
FT /note="SP -> GS (in Ref. 3; AAH66492)"
FT /evidence="ECO:0000305"
FT CONFLICT 128
FT /note="D -> A (in Ref. 3; AAH66492)"
FT /evidence="ECO:0000305"
FT CONFLICT 136
FT /note="I -> F (in Ref. 1; AAM90586)"
FT /evidence="ECO:0000305"
FT CONFLICT 138
FT /note="N -> K (in Ref. 1; AAM90586)"
FT /evidence="ECO:0000305"
FT CONFLICT 148
FT /note="S -> G (in Ref. 2; BAF63790)"
FT /evidence="ECO:0000305"
FT CONFLICT 185
FT /note="R -> S (in Ref. 3; AAH66492)"
FT /evidence="ECO:0000305"
FT CONFLICT 188
FT /note="N -> H (in Ref. 3; AAH66492)"
FT /evidence="ECO:0000305"
FT CONFLICT 307..322
FT /note="Missing (in Ref. 3; AAH66492)"
FT /evidence="ECO:0000305"
FT CONFLICT 339
FT /note="T -> S (in Ref. 3; AAH66492)"
FT /evidence="ECO:0000305"
FT CONFLICT 387
FT /note="K -> G (in Ref. 3; AAH66492)"
FT /evidence="ECO:0000305"
FT CONFLICT 391..398
FT /note="SIEGSNVT -> YFKGPYGK (in Ref. 3; AAH66492)"
FT /evidence="ECO:0000305"
FT CONFLICT 403
FT /note="V -> I (in Ref. 3; AAH66492)"
FT /evidence="ECO:0000305"
FT CONFLICT 472
FT /note="P -> S (in Ref. 3; AAH66492)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 488 AA; 53753 MW; 3C20951AD9A49FF1 CRC64;
MGNCFGSRGI CDRPKNTNIR LSLPAVCFVW QVSMIILFGV FVRYNEEADT NWVYTKKEKN
ITSDIENDFY FRYPSFQDVH VMIFVGFGFL MTFLKRYSFG AVGFNFLIAA FGLQWALLMQ
GWFSPLGDDG KIKIGIENLI NADFCVASCL IAYGAVLGKV SPVQLLVMTL FGITLYAVEE
FIILRVLNAK DAGGSMVIHT FGAYYGLSIS RVLYRPNLNK SKHMNGSVYH SDVFAMIGTL
FLWMFWPSFN SAICNHGDGQ HRAAINTYLA LASTVLTTVA ISSMFEKTGK LDMVHIQNST
LAGGVAVGTA AEFMLMPYGS LIVGFFCGII STLGYIYLTP FLEERLKIQD TCGIHNLHAM
PGVIGGIVGA ISAAAASKEV YGDLGLKNIF SIEGSNVTRL PTVQGGYQAA ALCVALCFGI
GGGTFVGLVL KLPIWGDPAD EHCFNDEMYW EVPEDEESII PPVLSYNNHM IPNNKHEEMR
ETNFAEQS
