RHD3_CANAL
ID RHD3_CANAL Reviewed; 204 AA.
AC Q5A5U4; A0A1D8PLZ0;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 25-MAY-2022, entry version 77.
DE RecName: Full=Cell wall protein RHD3;
DE AltName: Full=LDG family protein 7;
DE AltName: Full=Predicted GPI-anchored protein 29;
DE AltName: Full=Repressed during hyphae development protein 3;
DE Flags: Precursor;
GN Name=RHD3; Synonyms=LDG7, PGA29; OrderedLocusNames=CAALFM_C404050CA;
GN ORFNames=CaO19.12765, CaO19.5305;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP INDUCTION.
RX PubMed=12388749; DOI=10.1091/mbc.e02-05-0272;
RA Nantel A., Dignard D., Bachewich C., Harcus D., Marcil A., Bouin A.P.,
RA Sensen C.W., Hogues H., van het Hoog M., Gordon P., Rigby T., Benoit F.,
RA Tessier D.C., Thomas D.Y., Whiteway M.;
RT "Transcription profiling of Candida albicans cells undergoing the yeast-to-
RT hyphal transition.";
RL Mol. Biol. Cell 13:3452-3465(2002).
RN [5]
RP PREDICTION OF GPI-ANCHOR.
RX PubMed=12845604; DOI=10.1002/yea.1007;
RA De Groot P.W., Hellingwerf K.J., Klis F.M.;
RT "Genome-wide identification of fungal GPI proteins.";
RL Yeast 20:781-796(2003).
RN [6]
RP INDUCTION.
RX PubMed=15387822; DOI=10.1111/j.1365-2958.2004.04214.x;
RA Lan C.Y., Rodarte G., Murillo L.A., Jones T., Davis R.W., Dungan J.,
RA Newport G., Agabian N.;
RT "Regulatory networks affected by iron availability in Candida albicans.";
RL Mol. Microbiol. 53:1451-1469(2004).
RN [7]
RP INDUCTION.
RX PubMed=16455273; DOI=10.1016/j.fgb.2005.12.002;
RA Castillo L., Martinez A.I., Garcera A., Garcia-Martinez J.,
RA Ruiz-Herrera J., Valentin E., Sentandreu R.;
RT "Genomic response programs of Candida albicans following protoplasting and
RT regeneration.";
RL Fungal Genet. Biol. 43:124-134(2006).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=18712765; DOI=10.1002/pmic.200800110;
RA Castillo L., Calvo E., Martinez A.I., Ruiz-Herrera J., Valentin E.,
RA Lopez J.A., Sentandreu R.;
RT "A study of the Candida albicans cell wall proteome.";
RL Proteomics 8:3871-3881(2008).
RN [9]
RP FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, INDUCTION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=20533408; DOI=10.1002/yea.1790;
RA de Boer A.D., de Groot P.W., Weindl G., Schaller M., Riedel D.,
RA Diez-Orejas R., Klis F.M., de Koster C.G., Dekker H.L., Gross U., Bader O.,
RA Weig M.;
RT "The Candida albicans cell wall protein Rhd3/Pga29 is abundant in the yeast
RT form and contributes to virulence.";
RL Yeast 27:611-624(2010).
RN [10]
RP INDUCTION.
RX PubMed=21622905; DOI=10.1128/ec.05011-11;
RA Sorgo A.G., Heilmann C.J., Dekker H.L., Bekker M., Brul S., de Koster C.G.,
RA de Koning L.J., Klis F.M.;
RT "Effects of fluconazole on the secretome, the wall proteome, and wall
RT integrity of the clinical fungus Candida albicans.";
RL Eukaryot. Cell 10:1071-1081(2011).
RN [11]
RP INDUCTION.
RX PubMed=21602216; DOI=10.1099/mic.0.049395-0;
RA Heilmann C.J., Sorgo A.G., Siliakus A.R., Dekker H.L., Brul S.,
RA de Koster C.G., de Koning L.J., Klis F.M.;
RT "Hyphal induction in the human fungal pathogen Candida albicans reveals a
RT characteristic wall protein profile.";
RL Microbiology 157:2297-2307(2011).
RN [12]
RP INDUCTION, AND FUNCTION.
RX PubMed=24090751; DOI=10.1179/1973947813y.0000000091;
RA Elisabetta V., Giuseppina M., Furio S., Annarita S., Marisa C.,
RA Maurizio S., Bruno M., Angiolella L.;
RT "The cell wall protein Rhd3/Pga29 is over-expressed in Candida albicans
RT upon micafungin treatment.";
RL J. Chemother. 25:332-340(2013).
CC -!- FUNCTION: Component of the cell wall involved in virulence. Does not
CC seem to have a major role in maintaining cell wall integrity but plays
CC a role in the relationship between C.albicans and the host.
CC {ECO:0000269|PubMed:20533408, ECO:0000269|PubMed:24090751}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall. Membrane; Lipid-anchor, GPI-
CC anchor.
CC -!- INDUCTION: Down-regulated during yeast-to-hyphal transition and by
CC fluconazole. Induced during cell wall regeneration following
CC protoplasting and highly overexpressed after treatment with micafungin.
CC {ECO:0000269|PubMed:12388749, ECO:0000269|PubMed:15387822,
CC ECO:0000269|PubMed:16455273, ECO:0000269|PubMed:20533408,
CC ECO:0000269|PubMed:21602216, ECO:0000269|PubMed:21622905,
CC ECO:0000269|PubMed:24090751}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
CC -!- PTM: O-glycosylated by PMT1. {ECO:0000269|PubMed:20533408}.
CC -!- DISRUPTION PHENOTYPE: Leads to a significant reduction of cell wall
CC mannan and to decreased virulence with a diminished induction of host
CC pro-inflammatory cytokines. {ECO:0000269|PubMed:20533408}.
CC -!- SIMILARITY: Belongs to the SRP1/TIP1 family. {ECO:0000305}.
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DR EMBL; CP017626; AOW29155.1; -; Genomic_DNA.
DR RefSeq; XP_717108.1; XM_712015.1.
DR AlphaFoldDB; Q5A5U4; -.
DR STRING; 237561.Q5A5U4; -.
DR GeneID; 3641212; -.
DR KEGG; cal:CAALFM_C404050CA; -.
DR CGD; CAL0000190005; RHD3.
DR VEuPathDB; FungiDB:C4_04050C_A; -.
DR HOGENOM; CLU_083354_0_1_1; -.
DR InParanoid; Q5A5U4; -.
DR OMA; DDENNWI; -.
DR OrthoDB; 1611342at2759; -.
DR PHI-base; PHI:3508; -.
DR PRO; PR:Q5A5U4; -.
DR Proteomes; UP000000559; Chromosome 4.
DR GO; GO:0046658; C:anchored component of plasma membrane; IDA:CGD.
DR GO; GO:0009986; C:cell surface; IDA:CGD.
DR GO; GO:0005576; C:extracellular region; IDA:CGD.
DR GO; GO:0009277; C:fungal-type cell wall; IDA:CGD.
DR GO; GO:0030446; C:hyphal cell wall; IDA:CGD.
DR GO; GO:0030445; C:yeast-form cell wall; IDA:CGD.
DR GO; GO:0031505; P:fungal-type cell wall organization; IMP:CGD.
PE 1: Evidence at protein level;
KW Cell wall; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Secreted; Signal; Virulence.
FT SIGNAL 1..15
FT /evidence="ECO:0000255"
FT CHAIN 16..182
FT /note="Cell wall protein RHD3"
FT /id="PRO_0000424596"
FT PROPEP 183..204
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000424597"
FT LIPID 182
FT /note="GPI-anchor amidated glycine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 204 AA; 21474 MW; 00A2BFD68F7F34EA CRC64;
MKFLAILSLS SSALATISSI QLFAKSDDSK VDGLGLYSKH EGAAIDYLFL GKNGADLKYD
DEKKQIFQEL KTSSITVRQS FTLGGDVYEL GATDNFIPVT INKDGTLSFT GDDKVYASKN
VNDPYRYSES EYAVSNKKTD DSAPITIVAK FSDDKAAETS GVAQAASSSA GPAQASVSNF
EGAAGQNKLS YGVGMAAVVA GLVM
