RHD6_ARATH
ID RHD6_ARATH Reviewed; 298 AA.
AC Q9C707;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Transcription factor RHD6 {ECO:0000305};
DE AltName: Full=Basic helix-loop-helix protein 83 {ECO:0000303|PubMed:12679534};
DE Short=AtbHLH83 {ECO:0000303|PubMed:12679534};
DE Short=bHLH 83 {ECO:0000303|PubMed:12679534};
DE AltName: Full=Protein ROOT HAIR DEFECTIVE 6 {ECO:0000303|PubMed:17556585};
DE Short=AtRHD6 {ECO:0000303|PubMed:17556585};
DE AltName: Full=Transcription factor EN 112 {ECO:0000305};
DE AltName: Full=Transcription factor bHLH83 {ECO:0000305};
DE AltName: Full=bHLH transcription factor bHLH083 {ECO:0000303|PubMed:12679534};
GN Name=RHD6 {ECO:0000303|PubMed:17556585};
GN Synonyms=BHLH83 {ECO:0000303|PubMed:12679534}, EN112 {ECO:0000305};
GN OrderedLocusNames=At1g66470 {ECO:0000312|PROSITE:PS50888};
GN ORFNames=F28G11.9 {ECO:0000312|EMBL:AAG51154.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 22-298, TISSUE SPECIFICITY, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [5]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=17556585; DOI=10.1126/science.1142618;
RA Menand B., Yi K., Jouannic S., Hoffmann L., Ryan E., Linstead P.,
RA Schaefer D.G., Dolan L.;
RT "An ancient mechanism controls the development of cells with a rooting
RT function in land plants.";
RL Science 316:1477-1480(2007).
RN [8]
RP FUNCTION.
RX PubMed=20139979; DOI=10.1038/ng.529;
RA Yi K., Menand B., Bell E., Dolan L.;
RT "A basic helix-loop-helix transcription factor controls cell growth and
RT size in root hairs.";
RL Nat. Genet. 42:264-267(2010).
RN [9]
RP FUNCTION, INTERACTION WITH RSL1; TIFY10B/JAZ2; TIFY6A/JAZ4; TIFY5A/JAZ8;
RP TIFY7/JAZ9 AND TIFY9/JAZ10, SUBCELLULAR LOCATION, AND INDUCTION BY
RP JASMONATE.
RX PubMed=31988260; DOI=10.1105/tpc.19.00617;
RA Han X., Zhang M., Yang M., Hu Y.;
RT "Arabidopsis JAZ proteins interact with and suppress RHD6 transcription
RT factor to regulate jasmonate-stimulated root hair development.";
RL Plant Cell 32:1049-1062(2020).
CC -!- FUNCTION: Transcription factor that is specifically required for the
CC development of root hairs (PubMed:17556585). Acts with RSL1 to
CC positively regulate root hair development (PubMed:17556585). Acts
CC downstream of genes that regulate epidermal pattern formation, such as
CC GL2 (PubMed:17556585). Targets directly RSL4, another transcription
CC factor involved in the regulation of root hair elongation
CC (PubMed:20139979). Acts with RSL1 as transcription factor that
CC integrates a jasmonate (JA) signaling pathway that stimulates root hair
CC growth (PubMed:31988260). {ECO:0000269|PubMed:17556585,
CC ECO:0000269|PubMed:20139979, ECO:0000269|PubMed:31988260}.
CC -!- SUBUNIT: Homodimer (Probable). Forms heterodimers with RSL1
CC (PubMed:31988260). Interacts with TIFY10B/JAZ2, TIFY6A/JAZ4,
CC TIFY5A/JAZ8, TIFY7/JAZ9 and TIFY9/JAZ10 (PubMed:31988260).
CC {ECO:0000269|PubMed:31988260, ECO:0000305}.
CC -!- INTERACTION:
CC Q9C707; Q5XVH0: BHLH109; NbExp=3; IntAct=EBI-4424338, EBI-15193531;
CC Q9C707; Q93Y00: BHLH7; NbExp=3; IntAct=EBI-4424338, EBI-4442198;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:17556585, ECO:0000269|PubMed:31988260}.
CC -!- TISSUE SPECIFICITY: Expressed constitutively in flowers
CC (PubMed:12679534). Expressed in root epidermal hair cells
CC (PubMed:17556585). {ECO:0000269|PubMed:12679534,
CC ECO:0000269|PubMed:17556585}.
CC -!- INDUCTION: Induced by jasmonate (JA) treatment.
CC {ECO:0000269|PubMed:31988260}.
CC -!- DISRUPTION PHENOTYPE: Strong reduction in root hair number and density
CC in seedlings (PubMed:17556585). The double mutant seedlings rhd6-3 and
CC rsl1-1 do not develop root hairs (PubMed:17556585).
CC {ECO:0000269|PubMed:17556585}.
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DR EMBL; AC074025; AAG51154.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34512.1; -; Genomic_DNA.
DR EMBL; AY128310; AAM91513.1; -; mRNA.
DR EMBL; BT006320; AAP13428.1; -; mRNA.
DR EMBL; AF488615; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; C96690; C96690.
DR RefSeq; NP_176820.1; NM_105318.3.
DR AlphaFoldDB; Q9C707; -.
DR SMR; Q9C707; -.
DR BioGRID; 28186; 12.
DR IntAct; Q9C707; 12.
DR STRING; 3702.AT1G66470.1; -.
DR PaxDb; Q9C707; -.
DR PRIDE; Q9C707; -.
DR ProteomicsDB; 240766; -.
DR EnsemblPlants; AT1G66470.1; AT1G66470.1; AT1G66470.
DR GeneID; 842965; -.
DR Gramene; AT1G66470.1; AT1G66470.1; AT1G66470.
DR KEGG; ath:AT1G66470; -.
DR Araport; AT1G66470; -.
DR TAIR; locus:2028957; AT1G66470.
DR eggNOG; ENOG502QT4N; Eukaryota.
DR HOGENOM; CLU_072935_0_0_1; -.
DR InParanoid; Q9C707; -.
DR OMA; PPAISCG; -.
DR OrthoDB; 1411594at2759; -.
DR PhylomeDB; Q9C707; -.
DR PRO; PR:Q9C707; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C707; baseline and differential.
DR Genevisible; Q9C707; AT.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048766; P:root hair initiation; IMP:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..298
FT /note="Transcription factor RHD6"
FT /id="PRO_0000358775"
FT DOMAIN 201..250
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..58
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 157..213
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 201..214
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 215..250
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT COMPBIAS 8..28
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..58
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 157..207
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 298 AA; 32088 MW; 08858EA7C5215F70 CRC64;
MALVNDHPNE TNYLSKQNSS SSEDLSSPGL DQPDAAYAGG GGGGGSASSS STMNSDHQQH
QGFVFYPSGE DHHNSLMDFN GSSFLNFDHH ESFPPPAISC GGSSGGGGFS FLEGNNMSYG
FTNWNHQHHM DIISPRSTET PQGQKDWLYS DSTVVTTGSR NESLSPKSAG NKRSHTGEST
QPSKKLSSGV TGKTKPKPTT SPKDPQSLAA KNRRERISER LKILQELVPN GTKVDLVTML
EKAISYVKFL QVQVKVLATD EFWPAQGGKA PDISQVKDAI DAILSSSQRD RNSNLITN
