RHDF1_DROME
ID RHDF1_DROME Reviewed; 1429 AA.
AC Q76NQ1; E1NZB2;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Inactive rhomboid protein 1;
DE Short=iRhom;
DE AltName: Full=Rhomboid family protein rhomboid-5;
GN Name=rho-5; Synonyms=iRhom; ORFNames=CG33304;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 162-1429.
RA Carlson J., Booth B., Frise E., Sandler J., Wan K., Yu C., Celniker S.E.;
RL Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE,
RP AND TISSUE SPECIFICITY.
RX PubMed=21439629; DOI=10.1016/j.cell.2011.02.047;
RA Zettl M., Adrain C., Strisovsky K., Lastun V., Freeman M.;
RT "Rhomboid family pseudoproteases use the ER quality control machinery to
RT regulate intercellular signaling.";
RL Cell 145:79-91(2011).
CC -!- FUNCTION: Rhomboid protease-like protein which has no protease activity
CC but regulates the secretion of several ligands of the epidermal growth
CC factor receptor. Indirectly activates the epidermal growth factor
CC receptor signaling pathway and may thereby regulate sleep, cell
CC survival, proliferation and migration. {ECO:0000269|PubMed:21439629}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:21439629}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:21439629}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in the nervous system and in
CC brain. {ECO:0000269|PubMed:21439629}.
CC -!- DEVELOPMENTAL STAGE: Expressed exclusively in the central nervous
CC system of embryos. In the third instar larva, expression is also
CC restricted to neural tissue with specific expression behind the
CC morphogenetic furrow in the developing eye and in the optic lobes
CC within brain. {ECO:0000269|PubMed:21439629}.
CC -!- DISRUPTION PHENOTYPE: Flies display a severe decrease in daytime
CC activity pattern and an increase in sleeping periods.
CC {ECO:0000269|PubMed:21439629}.
CC -!- SIMILARITY: Belongs to the peptidase S54 family. {ECO:0000305}.
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DR EMBL; AE014134; AAS64674.1; -; Genomic_DNA.
DR EMBL; BT125661; ADN43898.1; -; mRNA.
DR RefSeq; NP_995679.1; NM_205957.3.
DR AlphaFoldDB; Q76NQ1; -.
DR BioGRID; 77793; 6.
DR STRING; 7227.FBpp0088796; -.
DR PaxDb; Q76NQ1; -.
DR EnsemblMetazoa; FBtr0089855; FBpp0088796; FBgn0041723.
DR GeneID; 2768944; -.
DR KEGG; dme:Dmel_CG33304; -.
DR UCSC; CG33304-RA; d. melanogaster.
DR CTD; 2768944; -.
DR FlyBase; FBgn0041723; rho-5.
DR VEuPathDB; VectorBase:FBgn0041723; -.
DR eggNOG; KOG2290; Eukaryota.
DR eggNOG; KOG2291; Eukaryota.
DR GeneTree; ENSGT00940000168598; -.
DR HOGENOM; CLU_004028_0_0_1; -.
DR InParanoid; Q76NQ1; -.
DR OMA; HERETAC; -.
DR OrthoDB; 65171at2759; -.
DR PhylomeDB; Q76NQ1; -.
DR BioGRID-ORCS; 2768944; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 2768944; -.
DR PRO; PR:Q76NQ1; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0041723; Expressed in insect adult head and 9 other tissues.
DR Genevisible; Q76NQ1; DM.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:FlyBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042059; P:negative regulation of epidermal growth factor receptor signaling pathway; IMP:UniProtKB.
DR GO; GO:0010841; P:positive regulation of circadian sleep/wake cycle, wakefulness; IMP:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0042058; P:regulation of epidermal growth factor receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0050708; P:regulation of protein secretion; IBA:GO_Central.
DR Gene3D; 1.20.1540.10; -; 1.
DR InterPro; IPR022764; Peptidase_S54_rhomboid_dom.
DR InterPro; IPR035952; Rhomboid-like_sf.
DR Pfam; PF01694; Rhomboid; 1.
DR SUPFAM; SSF144091; SSF144091; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Membrane; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1429
FT /note="Inactive rhomboid protein 1"
FT /id="PRO_0000408514"
FT TOPO_DOM 1..843
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 844..864
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 865..1099
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 1100..1120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1121..1131
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1132..1152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1153..1156
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 1157..1177
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1178..1186
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1187..1207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1208..1210
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 1211..1231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1232..1245
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1246..1266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1267..1429
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 560..579
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 740..766
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..36
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 560..574
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1429 AA; 157302 MW; 20C570E02BCFB68B CRC64;
MSSNGSDLGH NNRRNEKGNP DSVHSSMRGS MSSTRRKSIN FQNQLQLALE SNECDVMYCS
LSSGRFRAPN GENFPPHGGK LKLSPLEKYD DANRGVPPPS PAPNSDCFAT CVANQLPSQS
CSVGSAKPET SVMQKHGMAG NGMVGSGMSG NGMAANGTNY HQRGVSPNSR YRLERYRESQ
STPQSKLMDN MGGMPSAVPS VSATRLLLPS NVPLPLAQCE NYNAYLGSTV HTPVKRYVPT
PPPAMELYSD LSISSSPAAN LPTPAAAVAS SASTSASAMQ SQYANMPYNY RSKCCHSEAS
HGSLSRTSQT YASCSPACTS PSPAPSTSMS MVFSAASSCS PIMTATSSAR SGNHRGDDSD
SVIVVPPGGG AMAQIEAENP SGTCLHCNTV RRTTGVHQTT QTTGPISPVP ISLPVPMAMP
VMSGLNEQMP SQSLESSMVS VQAKRLEGGG NMAVPPGSNQ HQLYRSQMAS NPRLQHIHHQ
TQQHQSLQVL PQPQNHHLSC KKRIGMYMRR TTSQFFGVEP STEAADCALW QGRHRRLAIR
CFGMFDTELE YHMQQAIGGG NEDAGQSNGT NGNYAPDRPD ILPVQDAIGM DMTMSGDSRR
QKCYTNRDFL AGEFVERKAS VGYMFVAMVS YLVHMFNKRR PIQMHRVRCP WQWSRSFAPI
HVQSHSNQQT DADGCLTDGL EAIIDDEVFF DSPCEITTSA VNDESSDIGR QAAKPRPCAD
QSGVGVSVYM AERQQNGWRT SALNSGGNAT SDINLTGDQS SHQPGAAHIP LCSSVSSQMQ
PAMRSSHSTT STCNRGNRIT AQLLDGVLEN SRRPPLRCIK YFSVNDLDDR TDHRPFFTYW
INTVQVVVLI LSIICYGIAP IGIGSEQKTG QVLVTSLSLQ TVQHVEQRNL WIGPRNIDLV
HMGAKFAACM RRDIKITEVV TKTRRHERET ACCIRNDDSG CVQSSQAECS IRGLYPTKSI
STWKKWSPSE SGPGGRISGS VCGLDPKFCD APASIAPYEW PDDITKWPIC RKTNSFTQRY
RYKDHTSEHM VCEVIGHPCC TGLYGECRIT TREYCDFIKG YFHEEASLCS QISCLNNVCG
MFPFISVETP DQLYRLLTSL CMHAGILHLA ITLIFQHLFL ADLERLIGTV RTAIVYIMSG
FAGNLTSAIL VPHRPEVGPS ASLSGVVASL IALLVWMHWK YLHKPHIALF KLLLLCSVLV
GIGTLPYQLN FLGLLAGVIC GCLLTMSLVP FTTFSKYGRK KKINLIWTCV LFHVVVYTAM
IVTFYIHPSE FHSISFVDMF SNSNGYDNFT NADHHGVDVV SSNTRYSQTQ NSQYYYHHHS
DDIIRKSVTF TEKALVSHIL YPTAPRKTSA QQWQEVEYSR SFNHLSNYSD RIKKSIGNIS
KLKQVFTSPI RFSNKNNHSN LMTELTSVHS ENKQKYLGYI NNNTEFNVL
