RHG08_HUMAN
ID RHG08_HUMAN Reviewed; 464 AA.
AC P85298; A6ZJ79; A6ZJ80; O75983; O95695; Q96RW1; Q96RW2; Q9HA49; Q9HC46;
AC Q9NSG0; Q9NVX8; Q9NXL1; Q9UH20;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Rho GTPase-activating protein 8;
DE AltName: Full=Rho-type GTPase-activating protein 8;
GN Name=ARHGAP8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Mammary gland, and Teratocarcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10591208; DOI=10.1038/990031;
RA Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M.,
RA Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C.,
RA Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E.,
RA Bridgeman A.M., Buck D., Burgess J., Burrill W.D., Burton J., Carder C.,
RA Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G.,
RA Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V.,
RA Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M.,
RA Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A.,
RA Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C.,
RA Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E.,
RA Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F.,
RA Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M.,
RA Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A.,
RA Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D.,
RA Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y.,
RA Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S.,
RA Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E.,
RA Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L.,
RA Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L.,
RA Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N.,
RA Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A.,
RA Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L.,
RA Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P.,
RA Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P.,
RA Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q.,
RA Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J.,
RA Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J.,
RA Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D.,
RA Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T.,
RA Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P.,
RA Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K.,
RA Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R.,
RA Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L.,
RA McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J.,
RA Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E.,
RA Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P.,
RA Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y.,
RA Wright H.;
RT "The DNA sequence of human chromosome 22.";
RL Nature 402:489-495(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP ALTERNATIVE SPLICING (ISOFORMS 1; 2 AND 3), AND TISSUE SPECIFICITY.
RX PubMed=15225876; DOI=10.1016/j.gene.2004.01.025;
RA Johnstone C.N., Castellvi-Bel S., Chang L.M., Bessa X., Nakagawa H.,
RA Harada H., Sung R.K., Pique J.M., Castells A., Rustgi A.K.;
RT "ARHGAP8 is a novel member of the RHOGAP family related to
RT ARHGAP1/CDC42GAP/p50RHOGAP: mutation and expression analyses in colorectal
RT and breast cancers.";
RL Gene 336:59-71(2004).
CC -!- FUNCTION: GTPase activator for the Rho-type GTPases by converting them
CC to an inactive GDP-bound state. {ECO:0000250}.
CC -!- INTERACTION:
CC P85298-4; Q8WWM9: CYGB; NbExp=3; IntAct=EBI-9523517, EBI-6309037;
CC P85298-4; Q13526: PIN1; NbExp=25; IntAct=EBI-9523517, EBI-714158;
CC P85298-4; Q99961: SH3GL1; NbExp=3; IntAct=EBI-9523517, EBI-697911;
CC P85298-4; Q9H0C1: ZMYND12; NbExp=3; IntAct=EBI-9523517, EBI-12030590;
CC P85298-4; Q63932: Map2k2; Xeno; NbExp=5; IntAct=EBI-9523517, EBI-397724;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1;
CC IsoId=P85298-1, Q9NSG0-6;
CC Sequence=Displayed;
CC Name=2;
CC IsoId=P85298-2, Q9NSG0-7;
CC Sequence=VSP_001652, VSP_001656, VSP_001657;
CC Name=3;
CC IsoId=P85298-3; Sequence=VSP_028883;
CC Name=4;
CC IsoId=P85298-4; Sequence=VSP_001652;
CC Name=5;
CC IsoId=P85298-5; Sequence=VSP_001652, VSP_054100, VSP_054101;
CC -!- TISSUE SPECIFICITY: Highly expressed in kidney and placenta. Also
CC expressed in colon, skeletal muscle, small intestine, stomach, and
CC testis. Not detected in brain, liver or spleen. Overexpressed in the
CC majority of colorectal tumors examined. {ECO:0000269|PubMed:15225876}.
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DR EMBL; AK001306; BAA91614.1; -; mRNA.
DR EMBL; AK022305; BAB14008.1; -; mRNA.
DR EMBL; Z83838; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; Z93244; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; Z98743; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC067824; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS14060.2; -. [P85298-4]
DR CCDS; CCDS33664.1; -.
DR CCDS; CCDS56233.1; -. [P85298-5]
DR PIR; B59436; B59436.
DR RefSeq; NP_001017526.1; NM_001017526.1. [P85298-1]
DR RefSeq; NP_001185655.1; NM_001198726.1. [P85298-5]
DR RefSeq; NP_851852.2; NM_181335.2. [P85298-4]
DR AlphaFoldDB; P85298; -.
DR SMR; P85298; -.
DR BioGRID; 117278; 20.
DR CORUM; P85298; -.
DR IntAct; P85298; 11.
DR STRING; 9606.ENSP00000374424; -.
DR iPTMnet; P85298; -.
DR PhosphoSitePlus; P85298; -.
DR BioMuta; ARHGAP8; -.
DR DMDM; 160016276; -.
DR EPD; P85298; -.
DR jPOST; P85298; -.
DR MassIVE; P85298; -.
DR MaxQB; P85298; -.
DR PaxDb; P85298; -.
DR PeptideAtlas; P85298; -.
DR PRIDE; P85298; -.
DR ProteomicsDB; 1781; -.
DR ProteomicsDB; 57764; -.
DR ProteomicsDB; 57765; -. [P85298-2]
DR ProteomicsDB; 57766; -. [P85298-3]
DR ProteomicsDB; 57767; -. [P85298-4]
DR Antibodypedia; 66858; 16 antibodies from 9 providers.
DR DNASU; 23779; -.
DR Ensembl; ENST00000336963.8; ENSP00000337287.4; ENSG00000241484.10. [P85298-5]
DR Ensembl; ENST00000356099.11; ENSP00000348407.6; ENSG00000241484.10. [P85298-4]
DR Ensembl; ENST00000389774.6; ENSP00000374424.2; ENSG00000241484.10. [P85298-1]
DR GeneID; 23779; -.
DR KEGG; hsa:23779; -.
DR MANE-Select; ENST00000356099.11; ENSP00000348407.6; NM_181335.3; NP_851852.2. [P85298-4]
DR UCSC; uc003bfj.3; human.
DR CTD; 23779; -.
DR DisGeNET; 23779; -.
DR GeneCards; ARHGAP8; -.
DR HGNC; HGNC:677; ARHGAP8.
DR HPA; ENSG00000241484; Tissue enhanced (kidney, salivary gland).
DR MIM; 609405; gene.
DR neXtProt; NX_P85298; -.
DR OpenTargets; ENSG00000241484; -.
DR PharmGKB; PA24961; -.
DR VEuPathDB; HostDB:ENSG00000241484; -.
DR eggNOG; KOG4406; Eukaryota.
DR GeneTree; ENSGT00940000160758; -.
DR HOGENOM; CLU_030214_1_1_1; -.
DR InParanoid; P85298; -.
DR OMA; YKEFDRX; -.
DR PhylomeDB; P85298; -.
DR PathwayCommons; P85298; -.
DR Reactome; R-HSA-8980692; RHOA GTPase cycle.
DR SignaLink; P85298; -.
DR SIGNOR; P85298; -.
DR BioGRID-ORCS; 23779; 12 hits in 1062 CRISPR screens.
DR ChiTaRS; ARHGAP8; human.
DR GeneWiki; ARHGAP8; -.
DR GenomeRNAi; 23779; -.
DR Pharos; P85298; Tbio.
DR PRO; PR:P85298; -.
DR Proteomes; UP000005640; Chromosome 22.
DR RNAct; P85298; protein.
DR Bgee; ENSG00000241484; Expressed in right uterine tube and 119 other tissues.
DR ExpressionAtlas; P85298; baseline and differential.
DR Genevisible; P85298; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005096; F:GTPase activator activity; IDA:BHF-UCL.
DR GO; GO:2001136; P:negative regulation of endocytic recycling; IBA:GO_Central.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IDA:BHF-UCL.
DR GO; GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IBA:GO_Central.
DR CDD; cd00170; SEC14; 1.
DR Gene3D; 1.10.555.10; -; 1.
DR Gene3D; 3.40.525.10; -; 1.
DR InterPro; IPR001251; CRAL-TRIO_dom.
DR InterPro; IPR036865; CRAL-TRIO_dom_sf.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR Pfam; PF13716; CRAL_TRIO_2; 1.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SMART; SM00516; SEC14; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR SUPFAM; SSF52087; SSF52087; 2.
DR PROSITE; PS50191; CRAL_TRIO; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; GTPase activation; Reference proteome.
FT CHAIN 1..464
FT /note="Rho GTPase-activating protein 8"
FT /id="PRO_0000308161"
FT DOMAIN 13..199
FT /note="CRAL-TRIO"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00056"
FT DOMAIN 226..412
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 439..464
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 101..131
FT /note="Missing (in isoform 2, isoform 4 and isoform 5)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_001652"
FT VAR_SEQ 127..131
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_028883"
FT VAR_SEQ 325
FT /note="V -> A (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_001656"
FT VAR_SEQ 326..464
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_001657"
FT VAR_SEQ 326..336
FT /note="ESSLRVTGCRQ -> PGEHLQQNEQL (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_054100"
FT VAR_SEQ 337..464
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_054101"
FT VARIANT 172
FT /note="Y -> C (in dbSNP:rs16992915)"
FT /id="VAR_049138"
FT VARIANT 333
FT /note="G -> R (in dbSNP:rs6007344)"
FT /id="VAR_049139"
FT VARIANT 415
FT /note="P -> L (in dbSNP:rs2071762)"
FT /id="VAR_049140"
FT VARIANT 418
FT /note="P -> R (in dbSNP:rs9614957)"
FT /id="VAR_061182"
SQ SEQUENCE 464 AA; 53484 MW; F003A341AE5271D2 CRC64;
MAGQDPALST SHPFYDVARH GILQVAGDDR FGRRVVTFSC CRMPPSHELD HQRLLEYLKY
TLDQYVENDY TIVYFHYGLN SRNKPSLGWL QSAYKEFDRK DGDLTMWPRL VSNSKLKRSS
HLSLPKYWDY RYKKNLKALY VVHPTSFIKV LWNILKPLIS HKFGKKVIYF NYLSELHEHL
KYDQLVIPPE VLRYDEKLQS LHEGRTPPPT KTPPPRPPLP TQQFGVSLQY LKDKNQGELI
PPVLRFTVTY LREKGLRTEG LFRRSASVQT VREIQRLYNQ GKPVNFDDYG DIHIPAVILK
TFLRELPQPL LTFQAYEQIL GITCVESSLR VTGCRQILRS LPEHNYVVLR YLMGFLHAVS
RESIFNKMNS SNLACVFGLN LIWPSQGVSS LSALVPLNMF TELLIEYYEK IFSTPEAPGE
HGLAPWEQGS RAAPLQEAVP RTQATGLTKP TLPPSPLMAA RRRL
