RHG20_MOUSE
ID RHG20_MOUSE Reviewed; 1182 AA.
AC Q6IFT4; Q6ZPS4; Q8BLZ7; Q8BXI4;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Rho GTPase-activating protein 20;
DE AltName: Full=RA and RhoGAP domain-containing protein;
DE Short=RARhoGAP;
DE AltName: Full=Rho-type GTPase-activating protein 20;
GN Name=Arhgap20; Synonyms=Kiaa1391;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1-388 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Aorta, Cerebellum, and Vein;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 120-1182 (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [3]
RP IDENTIFICATION.
RX PubMed=15234003; DOI=10.1016/j.ygeno.2004.03.004;
RA Curry B.J., Su H., Law E.G., McLaughlin E.A., Nixon B., Aitken R.J.;
RT "Identification of RARhoGAP, a novel putative RhoGAP gene expressed in male
RT germ cells.";
RL Genomics 84:406-418(2004).
RN [4]
RP IDENTIFICATION.
RX PubMed=14532992;
RA Katoh M., Katoh M.;
RT "Identification and characterization of human KIAA1391 and mouse Kiaa1391
RT genes encoding novel RhoGAP family proteins with RA domain and ANXL
RT repeats.";
RL Int. J. Oncol. 23:1471-1476(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46; SER-704 AND SER-730, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: GTPase activator for the Rho-type GTPases by converting them
CC to an inactive GDP-bound state. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6IFT4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6IFT4-2; Sequence=VSP_024300, VSP_024301;
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DR EMBL; AK040756; BAC30694.1; -; mRNA.
DR EMBL; AK046916; BAC32919.1; -; mRNA.
DR EMBL; AK129345; BAC98155.1; -; mRNA.
DR EMBL; BK004077; DAA04568.1; -; mRNA.
DR CCDS; CCDS23177.1; -. [Q6IFT4-1]
DR RefSeq; NP_780744.2; NM_175535.3. [Q6IFT4-1]
DR AlphaFoldDB; Q6IFT4; -.
DR SMR; Q6IFT4; -.
DR STRING; 10090.ENSMUSP00000065633; -.
DR iPTMnet; Q6IFT4; -.
DR PhosphoSitePlus; Q6IFT4; -.
DR MaxQB; Q6IFT4; -.
DR PaxDb; Q6IFT4; -.
DR PRIDE; Q6IFT4; -.
DR ProteomicsDB; 255204; -. [Q6IFT4-1]
DR ProteomicsDB; 255205; -. [Q6IFT4-2]
DR Antibodypedia; 32015; 127 antibodies from 27 providers.
DR DNASU; 244867; -.
DR Ensembl; ENSMUST00000065496; ENSMUSP00000065633; ENSMUSG00000053199. [Q6IFT4-1]
DR GeneID; 244867; -.
DR KEGG; mmu:244867; -.
DR UCSC; uc009pli.1; mouse. [Q6IFT4-2]
DR UCSC; uc009plj.1; mouse. [Q6IFT4-1]
DR CTD; 57569; -.
DR MGI; MGI:2445175; Arhgap20.
DR VEuPathDB; HostDB:ENSMUSG00000053199; -.
DR eggNOG; KOG4724; Eukaryota.
DR GeneTree; ENSGT00940000154633; -.
DR HOGENOM; CLU_008526_0_0_1; -.
DR InParanoid; Q6IFT4; -.
DR OMA; IDDQHCK; -.
DR OrthoDB; 148399at2759; -.
DR PhylomeDB; Q6IFT4; -.
DR TreeFam; TF331062; -.
DR Reactome; R-MMU-8980692; RHOA GTPase cycle.
DR Reactome; R-MMU-9013148; CDC42 GTPase cycle.
DR Reactome; R-MMU-9013149; RAC1 GTPase cycle.
DR BioGRID-ORCS; 244867; 1 hit in 56 CRISPR screens.
DR ChiTaRS; Arhgap20; mouse.
DR PRO; PR:Q6IFT4; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q6IFT4; protein.
DR Bgee; ENSMUSG00000053199; Expressed in animal zygote and 188 other tissues.
DR ExpressionAtlas; Q6IFT4; baseline and differential.
DR Genevisible; Q6IFT4; MM.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.555.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR000159; RA_dom.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR Pfam; PF00788; RA; 1.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50200; RA; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; GTPase activation; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..1182
FT /note="Rho GTPase-activating protein 20"
FT /id="PRO_0000283087"
FT DOMAIN 85..185
FT /note="PH"
FT DOMAIN 194..283
FT /note="Ras-associating"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00166"
FT DOMAIN 365..551
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 745..772
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 803..839
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 935..955
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 982..1011
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1074..1101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1142..1182
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..26
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 752..771
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 993..1007
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1081..1097
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1151..1182
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 46
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 704
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 730
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 184..217
FT /note="IALEKEKDYPKSIPLKIFAKDIGNCAYFKTITVM -> VPVHTVLMLSLTCL
FT NQTCYCQICPRGAPFFLNGN (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024300"
FT VAR_SEQ 218..1182
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024301"
SQ SEQUENCE 1182 AA; 131374 MW; 39B0BC5CC944D50B CRC64;
MEAMSPQQDA LGAQPGRSSS LTGMSRIAGG PGTKKKMKTL AERRRSAPSL ILDKALQKRP
STRDSHSASI DTCAFLSSFM CSSRTLLIDG PVELKRGLQR QERHLFLFND LFVSAKIKYN
NNFKIKNKIR LTDMWTASCV EEVGEGNMNA QKSFVLGWPT VNFVATFSSP EQKDKWLSLL
QRYIALEKEK DYPKSIPLKI FAKDIGNCAY FKTITVMNSD TASEVINMSL QMLGITGSER
DYQLWVNSGK EAAPYPLIGH EYPYGIKMSH LRDTALLTQG SRDSASPSQL QEPFLMEQLP
REMQCQFILK PTRLATAQQL SDSSQKTFKR RRSIINWAFW RGSSTHLDNL PMSPTSPMPG
QLFGVSLPDL CENDNLPKPI LDMLSFLNQK GPLTKGIFRQ SANMKSCREL KEKLNSGIEV
HLDCESIFVI ASVLKDFLRN IPESIFSSDL YDHWVCVMDQ GNDEEKINII QRLLDQLPRA
NVVFLRYLFG VLHNIEQHSL SNQMTAFNLA VCIAPSILWP PASSSPELEN EFTKKVSLLI
QFLIENCCRV FGEEIASLLG ELSERSDREH TPDITCFQMN DSSYDSLENE LNEEADAPCS
DLVKKLGQGS RSMDSVLTLS DYDLEQPEVE GLLTLSNFDL DQSKEEHIPI KPPLEPKPVN
VFVGYRKVSL GEHARAPAGP GTLSCLPVAA ADAPKVLRRH RRSSEPSIDY LDTKLSYLRE
FYQKKLRKSS CDAVLSRKDE DYLKQTQPQK KGDKVCLKQS SVTGTDVSKR NTANENIKKK
SLSGHEGTQV TLFTKSKPVP ISVASYSHGS SQDHPRKQAF DADPCRFSPP HLTDAQKSSR
VQHRRCSEPS IDDQNYKLSY LRGIYSMKQN KASCEAGLLH GEDDYLRRHK SLQIEGQKLI
NQSLVMGIEV GKSSSSHQST EKVLPPRLNL CPRASYSSLS SPGSSPSGSS VSSQDSAFSQ
ISEHSVFTPT ETSSPIDCTF QTQRKQEELS SDFDSPSRLS GMPGPSMGQA SSHLAYLRKG
TTEQPSQMHS VTLHPSAWLR SGLVTLKNWS LKKKTKAARP EDRKVCSLKE PLELPSCASG
TPEADSLQES QDDLQGDEGP GQTACGFSSY ACQDSEQHAG SPFHLAESRL KPCMKLYKGE
ESGGQYPCDN PWEGASSSLE TTEDTANPGA EPTTFAMTGT DI
