RHG20_RAT
ID RHG20_RAT Reviewed; 1182 AA.
AC Q6REY9;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Rho GTPase-activating protein 20;
DE AltName: Full=RA and RhoGAP domain-containing protein;
DE Short=RARhoGAP;
DE AltName: Full=Rho-type GTPase-activating protein 20;
GN Name=Arhgap20;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=Wistar; TISSUE=Testis;
RX PubMed=15234003; DOI=10.1016/j.ygeno.2004.03.004;
RA Curry B.J., Su H., Law E.G., McLaughlin E.A., Nixon B., Aitken R.J.;
RT "Identification of RARhoGAP, a novel putative RhoGAP gene expressed in male
RT germ cells.";
RL Genomics 84:406-418(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: GTPase activator for the Rho-type GTPases by converting them
CC to an inactive GDP-bound state. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6REY9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6REY9-2; Sequence=VSP_024302;
CC -!- TISSUE SPECIFICITY: Highest expression is found in testis. Ubiquitously
CC expressed in extragonadal tissues. {ECO:0000269|PubMed:15234003}.
CC -!- DEVELOPMENTAL STAGE: Found to be expressed in developing spermatocytes,
CC but not in terminally differentiated spermatozoa.
CC {ECO:0000269|PubMed:15234003}.
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DR EMBL; AY501475; AAS77204.1; -; mRNA.
DR EMBL; AABR03065952; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03062969; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03064628; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AABR03062444; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_998794.1; NM_213629.1. [Q6REY9-2]
DR RefSeq; XP_006243109.1; XM_006243047.3. [Q6REY9-1]
DR AlphaFoldDB; Q6REY9; -.
DR SMR; Q6REY9; -.
DR STRING; 10116.ENSRNOP00000034071; -.
DR iPTMnet; Q6REY9; -.
DR PhosphoSitePlus; Q6REY9; -.
DR PaxDb; Q6REY9; -.
DR PRIDE; Q6REY9; -.
DR Ensembl; ENSRNOT00000035989; ENSRNOP00000034071; ENSRNOG00000025624. [Q6REY9-1]
DR Ensembl; ENSRNOT00000059078; ENSRNOP00000055856; ENSRNOG00000025624. [Q6REY9-2]
DR GeneID; 367085; -.
DR KEGG; rno:367085; -.
DR UCSC; RGD:1303160; rat. [Q6REY9-1]
DR CTD; 57569; -.
DR RGD; 1303160; Arhgap20.
DR eggNOG; KOG4724; Eukaryota.
DR GeneTree; ENSGT00940000154633; -.
DR HOGENOM; CLU_008526_0_0_1; -.
DR InParanoid; Q6REY9; -.
DR OMA; IDDQHCK; -.
DR OrthoDB; 148399at2759; -.
DR PhylomeDB; Q6REY9; -.
DR TreeFam; TF331062; -.
DR Reactome; R-RNO-8980692; RHOA GTPase cycle.
DR Reactome; R-RNO-9013148; CDC42 GTPase cycle.
DR Reactome; R-RNO-9013149; RAC1 GTPase cycle.
DR PRO; PR:Q6REY9; -.
DR Proteomes; UP000002494; Chromosome 8.
DR Bgee; ENSRNOG00000025624; Expressed in testis and 17 other tissues.
DR Genevisible; Q6REY9; RN.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0031267; F:small GTPase binding; TAS:RGD.
DR GO; GO:0035023; P:regulation of Rho protein signal transduction; TAS:RGD.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.555.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR000159; RA_dom.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR Pfam; PF00788; RA; 1.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50200; RA; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; GTPase activation; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..1182
FT /note="Rho GTPase-activating protein 20"
FT /id="PRO_0000283088"
FT DOMAIN 86..187
FT /note="PH"
FT DOMAIN 194..283
FT /note="Ras-associating"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00166"
FT DOMAIN 365..551
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 744..791
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 932..953
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 981..1009
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1140..1182
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..26
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 749..771
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 990..1009
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 46
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 704
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6IFT4"
FT MOD_RES 730
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6IFT4"
FT VAR_SEQ 1..36
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15234003"
FT /id="VSP_024302"
FT CONFLICT 284
FT /note="S -> F (in Ref. 1; AAS77204)"
FT /evidence="ECO:0000305"
FT CONFLICT 615
FT /note="S -> P (in Ref. 1; AAS77204)"
FT /evidence="ECO:0000305"
FT CONFLICT 637..638
FT /note="NF -> SL (in Ref. 1; AAS77204)"
FT /evidence="ECO:0000305"
FT CONFLICT 788
FT /note="I -> N (in Ref. 1; AAS77204)"
FT /evidence="ECO:0000305"
FT CONFLICT 960
FT /note="S -> C (in Ref. 1; AAS77204)"
FT /evidence="ECO:0000305"
FT CONFLICT 1005
FT /note="T -> M (in Ref. 1; AAS77204)"
FT /evidence="ECO:0000305"
FT CONFLICT 1099
FT /note="G -> E (in Ref. 1; AAS77204)"
FT /evidence="ECO:0000305"
FT CONFLICT 1104
FT /note="A -> T (in Ref. 1; AAS77204)"
FT /evidence="ECO:0000305"
FT CONFLICT 1134
FT /note="M -> I (in Ref. 1; AAS77204)"
FT /evidence="ECO:0000305"
FT CONFLICT 1156
FT /note="P -> T (in Ref. 1; AAS77204)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1182 AA; 131138 MW; 6D3EB56A11EB023E CRC64;
MEAMSPQQDA LGAQPGRSSS LTGMSRIAGG PGTKKKMKTL AERRRSAPSL ILDKALQKRP
STRDSHSASI DTCAFLSSFM CSSRTLLIDG PVELKRGLQR QERHLFLFND LFVSAKIKYN
NNFKIKNKVK LADMWTASCV DEVGEGNTNA LKSFVLGWPT VNFVATFSSP EQKDKWLSLL
QRYIALEKEK DYPKSIPLKI FAKDIGNCAY FKTISVMNSD TASEVINMSL QMLGITGSER
DYQLWVNSGK EAAPYPLIGH EYPYGIKMSH LRDTALLTQG SKDSASPSQL QEPFLMEQLP
REMQCQFILK PTRLATAQQL SDSSHKTYKR RRSIINWAFW RGSSTHLDNL PVSPTSPMPG
QLFGVSLPDI CENDNLPKPI LDMLSFLNQK GPLTKGIFRQ SANMKSCREL KEKLNSGIEV
HLDCESIFVI ASVLKDFLRN IPESIFSSDL YDHWVCVMDQ GNDEEKINTI QRLLDQLPRA
NVVFLRYLFG VLHNIEQHSL SNQMTAFNLA VCIAPSILWP PASSSPELEN EFTKKVSLLI
QFLIENCCRI FGEEITSLLG ELSERSDREH TPDTSCFQLN DSSYDSLENE LNEDADAPCS
DLVKRLGQGS RSMDSVLTLS DYDLEPPEAE GLLTLSNFDL DQSKEEHIRT KQPLETKPVS
VFVAYRKVSL GEHTRAPDGP GTPSCLPATA SDARKVFRRH RRSSEPSIDY LDAKLSYLRE
FYQKKLRKSS CDAVLSRKDE DYLKQTQPQK KGDQRCFKQS SVTGTDVSKR NTANENIKKK
SLSGHEGIQE TPFTKSKPVA ISVASYMSSQ DHSWEQPFEA DACRFSPPHI ADAQKSSRAH
RRCSEPSIDD QNYKLSYLRG VYSKKQSKTS CEAGLLHGEE DYLKRHKSLQ MEGQKLINQS
LVMGIEVGKS SSSTNQSTEK VLPPRLNLCP RASYSSLSSP GTSPSGSSVS SQDSAFSQIS
EHSVFTPTET SSPIDCTFRA QRKQEELSSD CDSPSLVSGM PGPSTGQASS HLAYLRKGTT
EQLPQMHSVT LHPSTWLRSG LVTLKNWSLK KKTKAARPED RKDCSLKEPL ELPACAAGTP
EADSLQESQE DIHLGVDEGA GQTACGLSSY ACQDSEQHAS SPFCLAGSRL TLGMKLHEGE
ESGGQYPCDN PWEGAPSGLE TSEDAANTGV EPATVCNDGD RH
