RHG28_MOUSE
ID RHG28_MOUSE Reviewed; 729 AA.
AC Q8BN58; Q3TSH7; Q3UR11; Q3UZQ7; Q69ZM1; Q6NY11;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Rho GTPase-activating protein 28;
DE AltName: Full=Rho-type GTPase-activating protein 28;
GN Name=Arhgap28; Synonyms=Kiaa1314;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Eye, Head, Muellerian duct, and Spinal ganglion;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=C57BL/6J; TISSUE=Egg;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 96-729.
RC TISSUE=Embryonic tail;
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-164, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=17525332; DOI=10.1126/science.1140321;
RA Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E.,
RA Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y.,
RA Gygi S.P., Elledge S.J.;
RT "ATM and ATR substrate analysis reveals extensive protein networks
RT responsive to DNA damage.";
RL Science 316:1160-1166(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: GTPase activator for the Rho-type GTPases by converting them
CC to an inactive GDP-bound state. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8BN58-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BN58-2; Sequence=VSP_023729, VSP_023730;
CC Name=3;
CC IsoId=Q8BN58-3; Sequence=VSP_023731;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE36698.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK087523; BAC39911.1; -; mRNA.
DR EMBL; AK133720; BAE21798.1; -; mRNA.
DR EMBL; AK141901; BAE24877.1; -; mRNA.
DR EMBL; AK162051; BAE36698.1; ALT_INIT; mRNA.
DR EMBL; BC066788; AAH66788.1; -; mRNA.
DR EMBL; AK173147; BAD32425.1; -; mRNA.
DR CCDS; CCDS28950.1; -. [Q8BN58-1]
DR CCDS; CCDS84329.1; -. [Q8BN58-2]
DR RefSeq; NP_001334339.1; NM_001347410.1. [Q8BN58-2]
DR RefSeq; NP_766552.3; NM_172964.4. [Q8BN58-1]
DR AlphaFoldDB; Q8BN58; -.
DR SMR; Q8BN58; -.
DR BioGRID; 234587; 16.
DR IntAct; Q8BN58; 6.
DR STRING; 10090.ENSMUSP00000024840; -.
DR iPTMnet; Q8BN58; -.
DR PhosphoSitePlus; Q8BN58; -.
DR MaxQB; Q8BN58; -.
DR PaxDb; Q8BN58; -.
DR PRIDE; Q8BN58; -.
DR ProteomicsDB; 253278; -. [Q8BN58-1]
DR ProteomicsDB; 253279; -. [Q8BN58-2]
DR ProteomicsDB; 253280; -. [Q8BN58-3]
DR Antibodypedia; 21924; 123 antibodies from 23 providers.
DR DNASU; 268970; -.
DR Ensembl; ENSMUST00000024840; ENSMUSP00000024840; ENSMUSG00000024043. [Q8BN58-1]
DR Ensembl; ENSMUST00000163865; ENSMUSP00000130960; ENSMUSG00000024043. [Q8BN58-2]
DR GeneID; 268970; -.
DR KEGG; mmu:268970; -.
DR UCSC; uc008dki.2; mouse. [Q8BN58-2]
DR UCSC; uc008dkj.2; mouse. [Q8BN58-1]
DR CTD; 79822; -.
DR MGI; MGI:2147003; Arhgap28.
DR VEuPathDB; HostDB:ENSMUSG00000024043; -.
DR eggNOG; KOG2200; Eukaryota.
DR GeneTree; ENSGT00940000158929; -.
DR HOGENOM; CLU_023268_2_0_1; -.
DR InParanoid; Q8BN58; -.
DR OMA; PNAESRC; -.
DR OrthoDB; 700544at2759; -.
DR PhylomeDB; Q8BN58; -.
DR TreeFam; TF314044; -.
DR Reactome; R-MMU-8980692; RHOA GTPase cycle.
DR BioGRID-ORCS; 268970; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Arhgap28; mouse.
DR PRO; PR:Q8BN58; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q8BN58; protein.
DR Bgee; ENSMUSG00000024043; Expressed in lumbar dorsal root ganglion and 145 other tissues.
DR ExpressionAtlas; Q8BN58; baseline and differential.
DR Genevisible; Q8BN58; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005096; F:GTPase activator activity; ISS:MGI.
DR GO; GO:1904425; P:negative regulation of GTP binding; IDA:MGI.
DR GO; GO:0051497; P:negative regulation of stress fiber assembly; IDA:MGI.
DR GO; GO:0030833; P:regulation of actin filament polymerization; IBA:GO_Central.
DR GO; GO:0051056; P:regulation of small GTPase mediated signal transduction; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.555.10; -; 1.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; GTPase activation; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..729
FT /note="Rho GTPase-activating protein 28"
FT /id="PRO_0000280477"
FT DOMAIN 384..581
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 1..78
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 180..234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 54..75
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 195..209
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 216..232
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 70
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 164
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17525332"
FT VAR_SEQ 1..50
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_023729"
FT VAR_SEQ 435
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_023730"
FT VAR_SEQ 679..729
FT /note="ENSHGSSEHIKMQNQRLYEVGGNIGQHCLDPDAYILDVYHINPHAEWVIKP
FT -> KNPLATKHVLRGFLCVPVLDRFLDCPVGQ (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023731"
FT CONFLICT 101
FT /note="T -> TAAA (in Ref. 3; BAD32425)"
FT /evidence="ECO:0000305"
FT CONFLICT 258
FT /note="H -> R (in Ref. 2; AAH66788)"
FT /evidence="ECO:0000305"
FT CONFLICT 334
FT /note="T -> A (in Ref. 2; AAH66788)"
FT /evidence="ECO:0000305"
FT CONFLICT 361
FT /note="A -> T (in Ref. 2; AAH66788)"
FT /evidence="ECO:0000305"
FT CONFLICT 473
FT /note="S -> Y (in Ref. 1; BAE21798)"
FT /evidence="ECO:0000305"
FT CONFLICT 534
FT /note="M -> V (in Ref. 1; BAE24877)"
FT /evidence="ECO:0000305"
FT CONFLICT 672
FT /note="I -> V (in Ref. 2; AAH66788)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 729 AA; 81795 MW; 8284CB8B0BFF559C CRC64;
MEVEDSGGVV LTAYHSHARS QPQGAEPRCA SRASHPLSRK SIPRCRRINR MLSNESLHPP
SFSRSNSQAS VDSSASMEEF LREIESIKES SVGASQEQPP TAAAAAAEVK PVDEGELEAE
WLQDVGLSTL ISGNEEEDGK ALLSTLTRTQ AAAVKKRYNT YTQTLRKKNK QPVRDVRDIF
GVSESPPSDS CEHATQLDGT KEEKDLPGVT KTSRPLPDDA SLSSTTLSNG AQDEEGGFVA
LQSGSVSILE AIPDIAVHTN GSADAEQSVQ STLSDDDYHG KNVPAEAEEL SFEVSYSEMV
TEMPDRNKWK KSDIKKEDYV LTKFIIQKTR FGLTETGDLS VEDMKKIRHL SLIELTAFFD
AFGIQLKRNK TERVRGRDNG IFGVPLTVLL DNDRKKDPAV KVPLVLQKFF QKVEESGLES
EGIFRLSGCT AKVKQYREEL DARFNADKFK WDKMCHREAA VMLKAFFREL PTSLFPVEYI
PAFISLMERG PDIKVQFQAL HLMVMALPDA NRDTAQALMA FFNKVIANES KNRMNLWNIS
TVMAPNLFFS RSKHSDCEEL LLANTATHII RLMLKYQKIL WKVPSFLITQ VRRMNEATML
LKKQLPSMKK LLRRKTLDRE VSILKTSKVP QKSPSSRRMS DVPEGVIRVH APLLSKVSMA
IQLNSQTKAK DILAKFQYEN SHGSSEHIKM QNQRLYEVGG NIGQHCLDPD AYILDVYHIN
PHAEWVIKP
