RHG30_MOUSE
ID RHG30_MOUSE Reviewed; 1101 AA.
AC Q640N3; E9QKX0; Q571I5;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=Rho GTPase-activating protein 30;
DE AltName: Full=Rho-type GTPase-activating protein 30;
GN Name=Arhgap30;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 188-1101 (ISOFORM 2).
RX PubMed=15449545; DOI=10.1093/dnares/11.2.127;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of FLJ genes: the
RT complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified
RT by screening of terminal sequences of cDNA clones randomly sampled from
RT size-fractionated libraries.";
RL DNA Res. 11:127-135(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-875, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-875 AND SER-996, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: GTPase-activating protein (GAP) for RAC1 and RHOA, but not
CC for CDC42. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with RHOU in a GTP-independent manner.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q640N3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q640N3-2; Sequence=VSP_023736;
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DR EMBL; AC087229; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC082573; AAH82573.1; -; mRNA.
DR EMBL; AK220204; BAD90129.1; -; mRNA.
DR CCDS; CCDS15494.1; -. [Q640N3-2]
DR RefSeq; NP_001005508.2; NM_001005508.2.
DR AlphaFoldDB; Q640N3; -.
DR SMR; Q640N3; -.
DR BioGRID; 230542; 50.
DR IntAct; Q640N3; 27.
DR STRING; 10090.ENSMUSP00000059389; -.
DR iPTMnet; Q640N3; -.
DR PhosphoSitePlus; Q640N3; -.
DR EPD; Q640N3; -.
DR jPOST; Q640N3; -.
DR MaxQB; Q640N3; -.
DR PaxDb; Q640N3; -.
DR PRIDE; Q640N3; -.
DR ProteomicsDB; 255334; -. [Q640N3-1]
DR ProteomicsDB; 255335; -. [Q640N3-2]
DR DNASU; 226652; -.
DR GeneID; 226652; -.
DR KEGG; mmu:226652; -.
DR CTD; 257106; -.
DR MGI; MGI:2684948; Arhgap30.
DR eggNOG; KOG1449; Eukaryota.
DR InParanoid; Q640N3; -.
DR OrthoDB; 1300981at2759; -.
DR Reactome; R-MMU-8980692; RHOA GTPase cycle.
DR Reactome; R-MMU-9013148; CDC42 GTPase cycle.
DR Reactome; R-MMU-9013149; RAC1 GTPase cycle.
DR Reactome; R-MMU-9013420; RHOU GTPase cycle.
DR BioGRID-ORCS; 226652; 6 hits in 72 CRISPR screens.
DR ChiTaRS; Arhgap30; mouse.
DR PRO; PR:Q640N3; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q640N3; protein.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IBA:GO_Central.
DR Gene3D; 1.10.555.10; -; 1.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasmic vesicle; GTPase activation;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..1101
FT /note="Rho GTPase-activating protein 30"
FT /id="PRO_0000280479"
FT DOMAIN 20..215
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT REGION 305..397
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 450..499
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 622..848
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 878..901
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 968..987
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1044..1076
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 305..323
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 344..367
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 651..848
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 578
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q7Z6I6"
FT MOD_RES 875
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 996
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 458..465
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15449545"
FT /id="VSP_023736"
FT CONFLICT 455
FT /note="S -> C (in Ref. 3; BAD90129)"
FT /evidence="ECO:0000305"
FT CONFLICT 458
FT /note="L -> S (in Ref. 3; BAD90129)"
FT /evidence="ECO:0000305"
FT CONFLICT 463..471
FT /note="PGSVGPLAS -> GPGLGPGPP (in Ref. 3; BAD90129)"
FT /evidence="ECO:0000305"
FT CONFLICT 684
FT /note="E -> V (in Ref. 3; BAD90129)"
FT /evidence="ECO:0000305"
FT CONFLICT 799
FT /note="S -> G (in Ref. 3; BAD90129)"
FT /evidence="ECO:0000305"
FT CONFLICT 801
FT /note="T -> I (in Ref. 3; BAD90129)"
FT /evidence="ECO:0000305"
FT CONFLICT 1016
FT /note="L -> R (in Ref. 3; BAD90129)"
FT /evidence="ECO:0000305"
FT CONFLICT 1030
FT /note="S -> N (in Ref. 3; BAD90129)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1101 AA; 120114 MW; F91D1F2B5B67EB9E CRC64;
MKSRQKGKKK GSSKERVFGC DLREHLQHSG QEVPQVLRSC AEFVQEYGVV DGIYRLSGVS
SNIQKLRQEF ETERKPDLRR DVYLQDIHCV SSLCKAYFRE LPDPLLTYRL YDKFAEAVAV
QLEPERLVKI LEVLQELPIQ NYRTLEFLMR HLVHMASFSA QTNMHARNLA IVWAPNLLRS
KDIEASGFNG TAAFMEVRVQ SIVVEFILTH VDQLFRGDSL SAGVDLESGW KSLPGARASG
SSEDLMPTSL PYHLPSILQA GDGPPQIRPY HTIIEIAEHK RKGSLKVRKW RSIFNLGRSG
HETKRKLPLR VEDREEKSSK GTLRPAKSMD SLSAAAGASD EPEGLVGSSS SQPSSLMPES
LESNSMEGEQ EPEAEAPGSA NSEPGTPRAG RSAVRALGSS RAERCAGVHI SDPYNVNLPL
HITSILSVPP NIISNVSLVR LTRGLECPAL QPRPSPALGP GPPGSVGPLA SDEKSEARSV
PGPLDDSSPA AMTPALEDSL SQEVQDSFSF LEDLSSSEPE WVGVEEREVA KAEAAGAAGA
AAFSLGEDDP GMGYLEELLR VGPQVEEFSV EPPLDDLSLD DTQYVLAPNC CSLDSAVSTP
DVEEDYGEEV FLSAYDDLSP LLGPKPINWE GVGSLEEEAA GCGKQPPTQD EEEQACSETR
QEKEAKPRST SDNREEAEAT PETEMEAGKA DAEGGEAERS QKVMDSFKEG SREELEAKEE
NSEGREVESI KETKDVEKII GEPGKDEERE IGREEGAEKG DDTPVDSDMD PEHVFQEDLV
LEESWEVVHK HEAEKGRESE TKELRRKSDL KSREDQGHSE DSGSPEEGDD RKEGVFSKEQ
KSIDVETEVM RGVGDHLEEG ALSEGPGVEL LRVDSTEEIN EQTSEMKQAP LQPSEPEGME
AEGQLNPETC DLYSCPCGSA GGVGMRLAST LVQVRQVRSV PVVPPKPQFA KMPSAMCSKI
HVAPASPCPR PGRLDGTPGE KAWGSRASWR NGGSLSFDAA VALARERQRT ESQGVLRTQT
CTGGGDYSLS SRTPPCSMIL AHSSRPLSCL ERPPEGTEGS EPRSRLSLPP RELHPVVPLV
APQRQTYAFE TQTNHGKDEG V
