RHLI_PSEAE
ID RHLI_PSEAE Reviewed; 201 AA.
AC P54291;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Acyl-homoserine-lactone synthase;
DE EC=2.3.1.184;
DE AltName: Full=Autoinducer synthesis protein RhlI;
GN Name=rhlI; Synonyms=vsmI; OrderedLocusNames=PA3476;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DSM 2659 / PG201;
RX PubMed=7604006; DOI=10.1073/pnas.92.14.6424;
RA Ochsner U.A., Reiser J.;
RT "Autoinducer-mediated regulation of rhamnolipid biosurfactant synthesis in
RT Pseudomonas aeruginosa.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:6424-6428(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=8522523; DOI=10.1128/jb.177.24.7155-7163.1995;
RA Brint J.M., Ohman D.E.;
RT "Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the
RT control of RhlR-RhlI, another set of regulators in strain PAO1 with
RT homology to the autoinducer-responsive LuxR-LuxI family.";
RL J. Bacteriol. 177:7155-7163(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=7494482; DOI=10.1111/j.1365-2958.1995.mmi_17020333.x;
RA Latifi A., Winson M.K., Foglino M., Bycroft B.W., Stewart G.S.A.B.,
RA Lazdunski A., Williams P.;
RT "Multiple homologues of LuxR and LuxI control expression of virulence
RT determinants and secondary metabolites through quorum sensing in
RT Pseudomonas aeruginosa PAO1.";
RL Mol. Microbiol. 17:333-343(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Required for the synthesis of BHL (N-butanoyl-L-homoserine
CC lactone), and HHL (N-hexanoyl-L-homoserine lactone) autoinducer
CC molecules which bind to RhlR and thus acts in elastase biosynthesis
CC regulation.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-[ACP] + S-adenosyl-L-methionine = an N-acyl-L-
CC homoserine lactone + H(+) + holo-[ACP] + S-methyl-5'-thioadenosine;
CC Xref=Rhea:RHEA:10096, Rhea:RHEA-COMP:9685, Rhea:RHEA-COMP:14125,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:55474,
CC ChEBI:CHEBI:59789, ChEBI:CHEBI:64479, ChEBI:CHEBI:138651;
CC EC=2.3.1.184;
CC -!- SIMILARITY: Belongs to the autoinducer synthase family.
CC {ECO:0000255|PROSITE-ProRule:PRU00533}.
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DR EMBL; U11811; AAA82725.1; -; Genomic_DNA.
DR EMBL; U40458; AAC44037.1; -; Genomic_DNA.
DR EMBL; U15644; AAA89074.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG06864.1; -; Genomic_DNA.
DR PIR; A83212; A83212.
DR PIR; S70173; S70173.
DR RefSeq; NP_252166.1; NC_002516.2.
DR RefSeq; WP_003113896.1; NZ_QZGE01000039.1.
DR AlphaFoldDB; P54291; -.
DR SMR; P54291; -.
DR STRING; 287.DR97_4445; -.
DR PaxDb; P54291; -.
DR PRIDE; P54291; -.
DR DNASU; 878967; -.
DR EnsemblBacteria; AAG06864; AAG06864; PA3476.
DR GeneID; 878967; -.
DR KEGG; pae:PA3476; -.
DR PATRIC; fig|208964.12.peg.3639; -.
DR PseudoCAP; PA3476; -.
DR HOGENOM; CLU_085711_4_0_6; -.
DR InParanoid; P54291; -.
DR OMA; GQEYDRY; -.
DR PhylomeDB; P54291; -.
DR BioCyc; MetaCyc:MON-14565; -.
DR BioCyc; PAER208964:G1FZ6-3544-MON; -.
DR BRENDA; 2.3.1.184; 5087.
DR PHI-base; PHI:6747; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0061579; F:N-acyl homoserine lactone synthase activity; TAS:PHI-base.
DR GO; GO:0120218; P:host interaction involved in quorum sensing; TAS:PHI-base.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR InterPro; IPR018311; Autoind_synth_CS.
DR InterPro; IPR001690; Autoind_synthase.
DR PANTHER; PTHR39322; PTHR39322; 1.
DR Pfam; PF00765; Autoind_synth; 1.
DR PRINTS; PR01549; AUTOINDCRSYN.
DR SUPFAM; SSF55729; SSF55729; 1.
DR PROSITE; PS00949; AUTOINDUCER_SYNTH_1; 1.
DR PROSITE; PS51187; AUTOINDUCER_SYNTH_2; 1.
PE 3: Inferred from homology;
KW Autoinducer synthesis; Quorum sensing; Reference proteome;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..201
FT /note="Acyl-homoserine-lactone synthase"
FT /id="PRO_0000210894"
FT CONFLICT 4
FT /note="L -> F (in Ref. 3; AAA89074)"
FT /evidence="ECO:0000305"
FT CONFLICT 62
FT /note="S -> G (in Ref. 1 and 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 83
FT /note="D -> E (in Ref. 1 and 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 193..201
FT /note="QGVPLSMAV -> AEPR (in Ref. 2; AAC44037)"
FT /evidence="ECO:0000305"
FT CONFLICT 194..196
FT /note="GVP -> RT (in Ref. 3; AAA89074)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 201 AA; 22630 MW; 120F95F29D5A2698 CRC64;
MIELLSESLE GLSAAMIAEL GRYRHQVFIE KLGWDVVSTS RVRDQEFDQF DHPQTRYIVA
MSRQGICGCA RLLPTTDAYL LKDVFAYLCS ETPPSDPSVW ELSRYAASAA DDPQLAMKIF
WSSLQCAWYL GASSVVAVTT TAMERYFVRN GVILQRLGPP QKVKGETLVA ISFPAYQERG
LEMLLRYHPE WLQGVPLSMA V
