RHOB_BOVIN
ID RHOB_BOVIN Reviewed; 196 AA.
AC Q3ZBW5;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Rho-related GTP-binding protein RhoB;
DE Flags: Precursor;
GN Name=RHOB;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Fetal liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Mediates apoptosis in neoplastically transformed cells after
CC DNA damage. Not essential for development but affects cell adhesion and
CC growth factor signaling in transformed cells. Plays a negative role in
CC tumorigenesis as deletion causes tumor formation. Involved in
CC intracellular protein trafficking of a number of proteins. Targets PKN1
CC to endosomes and is involved in trafficking of the EGF receptor from
CC late endosomes to lysosomes. Also required for stability and nuclear
CC trafficking of AKT1/AKT which promotes endothelial cell survival during
CC vascular development. Serves as a microtubule-dependent signal that is
CC required for the myosin contractile ring formation during cell cycle
CC cytokinesis. Required for genotoxic stress-induced cell death in breast
CC cancer cells (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with
CC ARGGEF3. Interacts with RTKN. Interacts with AKAP13. Interacts with
CC RIPOR1. {ECO:0000250|UniProtKB:P62745, ECO:0000250|UniProtKB:P62746,
CC ECO:0000250|UniProtKB:P62747}.
CC -!- SUBCELLULAR LOCATION: Late endosome membrane {ECO:0000250}; Lipid-
CC anchor {ECO:0000250}. Cell membrane {ECO:0000250}; Lipid-anchor
CC {ECO:0000250}. Nucleus {ECO:0000250}. Cleavage furrow {ECO:0000250}.
CC Note=Late endosomal membrane (geranylgeranylated form). Plasma membrane
CC (farnesylated form). Also detected at the nuclear margin and in the
CC nucleus. Translocates to the equatorial region before furrow formation
CC in a ECT2-dependent manner (By similarity). {ECO:0000250}.
CC -!- PTM: Prenylation specifies the subcellular location of RHOB. The
CC farnesylated form is localized to the plasma membrane while the
CC geranylgeranylated form is localized to the endosome (By similarity).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rho family.
CC {ECO:0000305}.
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DR EMBL; BC103067; AAI03068.1; -; mRNA.
DR RefSeq; NP_001071390.1; NM_001077922.1.
DR AlphaFoldDB; Q3ZBW5; -.
DR SMR; Q3ZBW5; -.
DR STRING; 9913.ENSBTAP00000054785; -.
DR PaxDb; Q3ZBW5; -.
DR PeptideAtlas; Q3ZBW5; -.
DR PRIDE; Q3ZBW5; -.
DR GeneID; 515118; -.
DR KEGG; bta:515118; -.
DR CTD; 388; -.
DR eggNOG; KOG0393; Eukaryota.
DR HOGENOM; CLU_041217_21_2_1; -.
DR InParanoid; Q3ZBW5; -.
DR OrthoDB; 1166960at2759; -.
DR TreeFam; TF300837; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0032154; C:cleavage furrow; ISS:UniProtKB.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0070301; P:cellular response to hydrogen peroxide; ISS:UniProtKB.
DR GO; GO:0071479; P:cellular response to ionizing radiation; ISS:UniProtKB.
DR GO; GO:0000281; P:mitotic cytokinesis; ISS:UniProtKB.
DR GO; GO:0043065; P:positive regulation of apoptotic process; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR003578; Small_GTPase_Rho.
DR PANTHER; PTHR24072; PTHR24072; 1.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51420; RHO; 1.
PE 2: Evidence at transcript level;
KW Angiogenesis; Apoptosis; Cell adhesion; Cell membrane;
KW Developmental protein; Differentiation; Endosome; GTP-binding; Lipoprotein;
KW Membrane; Methylation; Nucleotide-binding; Nucleus; Palmitate;
KW Phosphoprotein; Prenylation; Protein transport; Reference proteome;
KW Transport; Tumor suppressor.
FT CHAIN 1..193
FT /note="Rho-related GTP-binding protein RhoB"
FT /id="PRO_0000284908"
FT PROPEP 194..196
FT /note="Removed in mature form"
FT /evidence="ECO:0000250"
FT /id="PRO_0000284909"
FT MOTIF 34..42
FT /note="Effector region"
FT /evidence="ECO:0000255"
FT BINDING 12..19
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 59..63
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 117..120
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT MOD_RES 154
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:P62746"
FT MOD_RES 193
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250"
FT LIPID 189
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250"
FT LIPID 192
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250"
FT LIPID 193
FT /note="S-farnesyl cysteine; in plasma membrane form"
FT /evidence="ECO:0000250"
FT LIPID 193
FT /note="S-geranylgeranyl cysteine; in endosomal form"
FT /evidence="ECO:0000250"
SQ SEQUENCE 196 AA; 22123 MW; CCE6FD53AE00CD83 CRC64;
MAAIRKKLVV VGDGACGKTC LLIVFSKDEF PEVYVPTVFE NYVADIEVDG KQVELALWDT
AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWV PEVKHFCPNV PIILVANKKD
LRSDEHVRTE LARMKQEPVR TDDGRAMAVR IQAYDYLECS AKTKEGVREV FETATRAALQ
KRYGSQNGCI NCCKVL
