ID   RHPN2_DANRE             Reviewed;         683 AA.
AC   Q6TNR1; Q803B1;
DT   01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Rhophilin-2;
DE   AltName: Full=GTP-Rho-binding protein 2;
GN   Name=rhpn2;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney marrow;
RX   PubMed=15520368; DOI=10.1073/pnas.0407241101;
RA   Song H.-D., Sun X.-J., Deng M., Zhang G.-W., Zhou Y., Wu X.-Y., Sheng Y.,
RA   Chen Y., Ruan Z., Jiang C.-L., Fan H.-Y., Zon L.I., Kanki J.P., Liu T.X.,
RA   Look A.T., Chen Z.;
RT   "Hematopoietic gene expression profile in zebrafish kidney marrow.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AB;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Binds specifically to GTP-Rho. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with RhoA. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RHPN family. {ECO:0000305}.
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DR   EMBL; AY391456; AAQ91268.1; -; mRNA.
DR   EMBL; BC044556; AAH44556.1; -; mRNA.
DR   RefSeq; NP_957472.1; NM_201178.1.
DR   AlphaFoldDB; Q6TNR1; -.
DR   SMR; Q6TNR1; -.
DR   STRING; 7955.ENSDARP00000024226; -.
DR   PaxDb; Q6TNR1; -.
DR   PRIDE; Q6TNR1; -.
DR   GeneID; 321330; -.
DR   KEGG; dre:321330; -.
DR   CTD; 85415; -.
DR   ZFIN; ZDB-GENE-030131-9927; rhpn2.
DR   eggNOG; KOG2220; Eukaryota.
DR   InParanoid; Q6TNR1; -.
DR   OrthoDB; 641122at2759; -.
DR   PhylomeDB; Q6TNR1; -.
DR   Reactome; R-DRE-5666185; RHO GTPases Activate Rhotekin and Rhophilins.
DR   Reactome; R-DRE-8980692; RHOA GTPase cycle.
DR   PRO; PR:Q6TNR1; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051497; P:negative regulation of stress fiber assembly; IBA:GO_Central.
DR   GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR   Gene3D; 1.25.40.280; -; 1.
DR   Gene3D; 2.30.42.10; -; 1.
DR   InterPro; IPR004328; BRO1_dom.
DR   InterPro; IPR038499; BRO1_sf.
DR   InterPro; IPR011072; HR1_rho-bd.
DR   InterPro; IPR036274; HR1_rpt_sf.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR041489; PDZ_6.
DR   InterPro; IPR036034; PDZ_sf.
DR   Pfam; PF03097; BRO1; 1.
DR   Pfam; PF02185; HR1; 1.
DR   Pfam; PF17820; PDZ_6; 1.
DR   SMART; SM01041; BRO1; 1.
DR   SMART; SM00742; Hr1; 1.
DR   SMART; SM00228; PDZ; 1.
DR   SUPFAM; SSF46585; SSF46585; 1.
DR   SUPFAM; SSF50156; SSF50156; 1.
DR   PROSITE; PS51180; BRO1; 1.
DR   PROSITE; PS50106; PDZ; 1.
DR   PROSITE; PS51860; REM_1; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Cytoplasm; Reference proteome.
FT   CHAIN           1..683
FT                   /note="Rhophilin-2"
FT                   /id="PRO_0000218900"
FT   DOMAIN          26..100
FT                   /note="REM-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01207"
FT   DOMAIN          111..460
FT                   /note="BRO1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00526"
FT   DOMAIN          515..593
FT                   /note="PDZ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT   REGION          649..683
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        254
FT                   /note="R -> G (in Ref. 2; AAH44556)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        258
FT                   /note="F -> L (in Ref. 2; AAH44556)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        280
FT                   /note="L -> F (in Ref. 2; AAH44556)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        288
FT                   /note="H -> Q (in Ref. 2; AAH44556)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        430
FT                   /note="C -> R (in Ref. 2; AAH44556)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   683 AA;  77057 MW;  9B459E94D9F380AB CRC64;
     MTDTLIPNGC KSNGPVENGY FKKGCNPFAQ TGRSKLQNKR AVLNQKIIKQ MRMRAGAENL
     LKATSNNKVR EQVVLELSYV NSDLQLLMEQ LEGLNSSVEV YQNVQESSSI PLIPLGLKET
     KDVDFSVPLK DFILEHYSED GSNFQNQIDD LMDLRQACRT PSRNNSGVDL IANYFSQLSF
     LETRFFSPTR QIGIFFTWYD SFTGMPVCQN NISLEKASML FNMAALYSQI GTRADRQTLA
     GLEDAIAAFQ KSARVLHFLK ETFTHTPSYD MSPAMLSMLL RLMLAQAHEC LFEQITLPGI
     RNEFFCLLKM AQEAAKVAET YAQVHQSMIE TPITKNVPFF WTTMSQLKIN HYNSLAHYFV
     STALLDHQLN PSDDEDKQEK ALSQLYDAMP EGRSPLDILK NKDERRRIGK AHLQRSIMGH
     EEAIRTHCRC RHLQKLDILS DILRASLNRS LTKFEQNDKE DEFTDYMLAP DIISKTEKKA
     EMEIPAATKV KVTDLFQRLG PLSVFSAKQR WTAPRTIRLI LQDRDLGFTL KGDAPVQIQS
     LDPLCPAAAG GLKEGDYLVA VGDTDCKWMG VSDVMKLLKD VDEEGINIRV VSMMDSSSQP
     MATKSATYCG GLSKTYSMIC LAHDEDDKNP KSRKVAKKPS FLSFGLKNKK SSSTLSLPNA
     DYSRPWNRPS ANYSNSHHES GLY