RHSA_DICD3
ID RHSA_DICD3 Reviewed; 1434 AA.
AC E0SAK8;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 1.
DT 03-AUG-2022, entry version 55.
DE RecName: Full=Probable deoxyribonuclease RhsA;
DE EC=3.1.-.-;
DE AltName: Full=DNase RhsA;
DE AltName: Full=Toxin RhsA;
GN Name=rhsA; OrderedLocusNames=Dda3937_01758;
OS Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Pectobacteriaceae; Dickeya.
OX NCBI_TaxID=198628;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3937;
RX PubMed=21217001; DOI=10.1128/jb.01513-10;
RA Glasner J.D., Yang C.H., Reverchon S., Hugouvieux-Cotte-Pattat N.,
RA Condemine G., Bohin J.P., Van Gijsegem F., Yang S., Franza T., Expert D.,
RA Plunkett G. III, San Francisco M.J., Charkowski A.O., Py B., Bell K.,
RA Rauscher L., Rodriguez-Palenzuela P., Toussaint A., Holeva M.C., He S.Y.,
RA Douet V., Boccara M., Blanco C., Toth I., Anderson B.D., Biehl B.S.,
RA Mau B., Flynn S.M., Barras F., Lindeberg M., Birch P.R., Tsuyumu S.,
RA Shi X., Hibbing M., Yap M.N., Carpentier M., Dassa E., Umehara M.,
RA Kim J.F., Rusch M., Soni P., Mayhew G.F., Fouts D.E., Gill S.R.,
RA Blattner F.R., Keen N.T., Perna N.T.;
RT "Genome sequence of the plant-pathogenic bacterium Dickeya dadantii 3937.";
RL J. Bacteriol. 193:2076-2077(2011).
RN [2]
RP PROBABLE FUNCTION AS A DNASE, FUNCTION AS A TOXIN, EXPRESSION IN E.COLI,
RP AND DISRUPTION PHENOTYPE.
RC STRAIN=3937;
RX PubMed=23572593; DOI=10.1073/pnas.1300627110;
RA Koskiniemi S., Lamoureux J.G., Nikolakakis K.C., t'Kint de Roodenbeke C.,
RA Kaplan M.D., Low D.A., Hayes C.S.;
RT "Rhs proteins from diverse bacteria mediate intercellular competition.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:7032-7037(2013).
CC -!- FUNCTION: Toxic component of a toxin-immunity protein module, which
CC functions as a cellular contact-dependent growth inhibition (CDI)
CC system. This protein may be a nuclease that is specifically inhibited
CC by its cognate immunity protein RhsAI. Upon expression of the C-
CC terminus (residues 1284-1434) in E.coli growth is inhibited, cells
CC elongate, nucleoids condense and plasmid DNA is degraded; these effects
CC are blocked specifically by cognate immunity protein RshIA. Cell
CC contact is necessary for growth inhibition.
CC {ECO:0000269|PubMed:23572593}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: A double rhsA-rhsIA deletion is outcompeted by
CC wild-type cells, restoration of rhsIA restores normal growth in
CC competition experiments. Restoration of growth requires the RhsA-
CC specific immunity protein, rhsIB does not restore growth.
CC {ECO:0000269|PubMed:23572593}.
CC -!- SIMILARITY: Belongs to the RHS/WapA nuclease family. {ECO:0000305}.
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DR EMBL; CP002038; ADM97066.1; -; Genomic_DNA.
DR RefSeq; WP_013316542.1; NC_014500.1.
DR AlphaFoldDB; E0SAK8; -.
DR SMR; E0SAK8; -.
DR STRING; 198628.Dda3937_01758; -.
DR EnsemblBacteria; ADM97066; ADM97066; Dda3937_01758.
DR GeneID; 9732267; -.
DR KEGG; ddd:Dda3937_01758; -.
DR eggNOG; COG3209; Bacteria.
DR eggNOG; COG4104; Bacteria.
DR HOGENOM; CLU_001218_1_8_6; -.
DR OMA; DGKGTHR; -.
DR OrthoDB; 1639867at2; -.
DR BioCyc; DDAD198628:DDA3937_RS04095-MON; -.
DR Proteomes; UP000006859; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR045351; DUF6531.
DR InterPro; IPR044927; Endonuclea_NS_2.
DR InterPro; IPR008727; PAAR_motif.
DR InterPro; IPR022385; Rhs_assc_core.
DR InterPro; IPR031325; RHS_repeat.
DR InterPro; IPR006530; YD.
DR Pfam; PF20148; DUF6531; 1.
DR Pfam; PF13930; Endonuclea_NS_2; 1.
DR Pfam; PF05488; PAAR_motif; 1.
DR Pfam; PF05593; RHS_repeat; 3.
DR TIGRFAMs; TIGR03696; Rhs_assc_core; 1.
DR TIGRFAMs; TIGR01643; YD_repeat_2x; 9.
PE 1: Evidence at protein level;
KW Hydrolase; Membrane; Nuclease; Reference proteome; Repeat; Toxin;
KW Transmembrane; Transmembrane helix; Virulence.
FT CHAIN 1..1434
FT /note="Probable deoxyribonuclease RhsA"
FT /id="PRO_0000423972"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 486..521
FT /note="YD 1"
FT /evidence="ECO:0000255"
FT REPEAT 592..628
FT /note="YD 2"
FT /evidence="ECO:0000255"
FT REPEAT 847..876
FT /note="YD 3"
FT /evidence="ECO:0000255"
FT REGION 14..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1434 AA; 160780 MW; C91E5148697E8391 CRC64;
MLNDILSRVA RVGAMHAGNR PNPPDDRPQP CRGKPPTSPG KTIKHKSFLG ALAGAVAGAL
VAAAVAAAAV FLVGVTGGLA VAAVGALAVF AAGDLISAVT NKVSAMVDSA SPAFGPVASG
SGNVFVEKQP VARATKDTVA CTKHNSPQLI AQGSESVFVN DAPAARIDDK TVCGATVKEG
ASTVFFGSGQ GTYLDIADEF SWWEKALLIA VEFLVPPSRG MLKGLGKLFI RGPKAVLRGS
RAGAKWIAGR LADKSSCASK AFKASSGLTR AKAAVKAFLK DPVYIASGEV IESRTDIELG
QTLPLAFERT YRSASVHIGL LGRGWHDSWS EVATVTRDGL NTHVVITLAQ GYDIDFTFHQ
DVQAVYCPHY PEFTLHRRGD GFSLWHRDQQ TWRDFSVVQG ERRLLSAIHD SHDNRIELVR
DPKGYLRQVR HSDGVTLLLV WQGEFLHQIQ RIDGGQKTLL AEYRQDEQGR LVEANATQAY
HLYYDYDAAH RLTRWHDNDQ TWARYEYDAQ GRCVYTTCAD GFLTARFDYL PDRVVMTDGL
GQCSEFGFND LFLMSWEKSP LGHVTRYEYD DYGNLLREIS PAGRVVEFTY LDDTGRVSTF
TDASGHQWQY DYDAAQRLCG VTDPLGREWG WMYDAEGNPE RLTGPDASEV RFTWNRYGLL
TQVSDAAGEV QARLQYDHRQ RLLSATDAES RTRQLRYDGQ DRVVQWQRAD GARFRLGYRR
ASWTLPEQLI RPDDKEEQRQ YDRHNNLLSY VDGNGALWRQ TFGPFDLLTA RTDAEGRTWH
YAYDKESQQL TTVIAPDGSH WQWWLDADGR VIRERDMTGT ETHYDYDEDG LCIRVRNGEG
DTRHFLYDAR GLLLRETAPD DTLHYRYDAV GRLTEVSSST AHVQLEYDLR DRMVREWHNG
TLLTRQVDDA ARTVTRTLTW DGDADDTINA LAPLTSLFHY TRTGELRQVQ LPDGADLTLT
HDAAGRESHR TGGSGFVQQR EYDVMGWLTR EMSGAQHDGH LLATQTREYR YDGAGNLTGV
RHNRDAEGYR LDATGRVQEI LSGGAGKPVD TTARFLYTRT GLPQEAGRLT EWQAGRLVQH
DDTHYQYDRA GRLIRKQVVQ PGYRPQVWQY RWDSRNQLRV VDTPNGERWL YRYDPFGRRV
GKRCDQKAEE IRYLWDGDQI AEIRHYRHGQ LIQRRHWVYN GWELVVQQRQ HTGGDWETDF
VTSSQNGTPQ ALFTPDGTLR WQAPKATLWG QRQAEKSESP DPGLAFAGQL RDSESGLCYN
RFRYYDPAGG CYVSPDPIGI AGGESNYGYV SNPMCWVDPF GLAKCPTLAH GANGEILSAK
ATVSKAELRT GSGTNQSSRD YARSLGNQTD DAGHILGNVL GGQGGKGNVF PQLPAINRGQ
YRDFEKVVKD YIGQHGSVDI EWAFKYGNGG TRPTEIYYDV YQNGQKVFGR IFNN
