RHSB_DICD3
ID RHSB_DICD3 Reviewed; 1436 AA.
AC E0SIS2;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 1.
DT 25-MAY-2022, entry version 50.
DE RecName: Full=Probable deoxyribonuclease RhsB;
DE EC=3.1.-.-;
DE AltName: Full=DNase RhsB;
DE AltName: Full=Toxin RhsB;
GN Name=rhsB; OrderedLocusNames=Dda3937_02773;
OS Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Pectobacteriaceae; Dickeya.
OX NCBI_TaxID=198628;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3937;
RX PubMed=21217001; DOI=10.1128/jb.01513-10;
RA Glasner J.D., Yang C.H., Reverchon S., Hugouvieux-Cotte-Pattat N.,
RA Condemine G., Bohin J.P., Van Gijsegem F., Yang S., Franza T., Expert D.,
RA Plunkett G. III, San Francisco M.J., Charkowski A.O., Py B., Bell K.,
RA Rauscher L., Rodriguez-Palenzuela P., Toussaint A., Holeva M.C., He S.Y.,
RA Douet V., Boccara M., Blanco C., Toth I., Anderson B.D., Biehl B.S.,
RA Mau B., Flynn S.M., Barras F., Lindeberg M., Birch P.R., Tsuyumu S.,
RA Shi X., Hibbing M., Yap M.N., Carpentier M., Dassa E., Umehara M.,
RA Kim J.F., Rusch M., Soni P., Mayhew G.F., Fouts D.E., Gill S.R.,
RA Blattner F.R., Keen N.T., Perna N.T.;
RT "Genome sequence of the plant-pathogenic bacterium Dickeya dadantii 3937.";
RL J. Bacteriol. 193:2076-2077(2011).
RN [2]
RP PROBABLE FUNCTION AS A DNASE, FUNCTION AS A TOXIN, EXPRESSION IN E.COLI,
RP AND DISRUPTION PHENOTYPE.
RC STRAIN=3937;
RX PubMed=23572593; DOI=10.1073/pnas.1300627110;
RA Koskiniemi S., Lamoureux J.G., Nikolakakis K.C., t'Kint de Roodenbeke C.,
RA Kaplan M.D., Low D.A., Hayes C.S.;
RT "Rhs proteins from diverse bacteria mediate intercellular competition.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:7032-7037(2013).
CC -!- FUNCTION: Toxic component of a toxin-immunity protein module, which
CC functions as a cellular contact-dependent growth inhibition (CDI)
CC system. This protein may be a nuclease that is specifically inhibited
CC by its cognate immunity protein RhsBI. Upon expression of the C-
CC terminus (residues 1284-1436) in E.coli growth is inhibited, cells
CC elongate, nucleoids condense and plasmid DNA is degraded; these effects
CC are blocked specifically by cognate immunity protein RshIB. Cell
CC contact is necessary for growth inhibition.
CC {ECO:0000269|PubMed:23572593}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: A double rhsB-rhsIB deletion is outcompeted by
CC wild-type cells, restoration of rhsIB restores normal growth in
CC competition experiments. Restoration of growth requires the RhsB-
CC specific immunity protein, rhsIA does not restore growth.
CC {ECO:0000269|PubMed:23572593}.
CC -!- SIMILARITY: Belongs to the RHS/WapA nuclease family. {ECO:0000305}.
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DR EMBL; CP002038; ADM99131.1; -; Genomic_DNA.
DR AlphaFoldDB; E0SIS2; -.
DR SMR; E0SIS2; -.
DR STRING; 198628.Dda3937_02773; -.
DR PRIDE; E0SIS2; -.
DR EnsemblBacteria; ADM99131; ADM99131; Dda3937_02773.
DR KEGG; ddd:Dda3937_02773; -.
DR eggNOG; COG3209; Bacteria.
DR eggNOG; COG4104; Bacteria.
DR HOGENOM; CLU_001218_1_8_6; -.
DR OMA; EGRTWRY; -.
DR Proteomes; UP000006859; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR CDD; cd00085; HNHc; 1.
DR InterPro; IPR045351; DUF6531.
DR InterPro; IPR003615; HNH_nuc.
DR InterPro; IPR008727; PAAR_motif.
DR InterPro; IPR022385; Rhs_assc_core.
DR InterPro; IPR031325; RHS_repeat.
DR InterPro; IPR006530; YD.
DR Pfam; PF20148; DUF6531; 1.
DR Pfam; PF05488; PAAR_motif; 1.
DR Pfam; PF05593; RHS_repeat; 6.
DR TIGRFAMs; TIGR03696; Rhs_assc_core; 1.
DR TIGRFAMs; TIGR01643; YD_repeat_2x; 8.
PE 1: Evidence at protein level;
KW Hydrolase; Membrane; Nuclease; Reference proteome; Repeat; Toxin;
KW Transmembrane; Transmembrane helix; Virulence.
FT CHAIN 1..1436
FT /note="Probable deoxyribonuclease RhsB"
FT /id="PRO_0000423973"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 486..521
FT /note="YD 1"
FT /evidence="ECO:0000255"
FT REPEAT 569..605
FT /note="YD 2"
FT /evidence="ECO:0000255"
FT REPEAT 612..647
FT /note="YD 3"
FT /evidence="ECO:0000255"
FT REPEAT 766..799
FT /note="YD 4"
FT /evidence="ECO:0000255"
FT REPEAT 847..879
FT /note="YD 5"
FT /evidence="ECO:0000255"
FT REGION 16..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1436 AA; 161673 MW; 72D014EAC5944262 CRC64;
MLNDILSRVA RVGAMHAGNR PNPPADRPQP CQGKPPTSPG KTIKHKSFLG ALAGAVAGAL
VAAAVAAAAV FLVGVTGGLA VAAVGALAVF AAGDLISAVT NKVSAVVDSA SPAFGPVASG
SGNVFVEKQP VARATKDTVA CTKHNSPQLI AQGSESVFVN DAPAARIDDK TVCGATLKEG
ASTVFFGSGQ GTYLEIADEF SWWEKALLIA VEFLVPPSRG MLKGLGKLFT RNGLKSVLKG
AKAGALFITK VPGKMGCAAR AFKANKGMAR FKEAAKAFKK DPVYLASGEV IESRTDIELG
QTLPLVFERT YRSASAHTGL LGRGWHDSWS EVATVTHDGL NTHVVITLAQ GYDIDFTFHQ
DVQAVYCPHY PEFTLHRRGD GFSLWHRDQQ TWRDFSVVQG ERRLLSAIHD SHDNRIELVR
DPKGYLRQLR HSDGVTLLLV WQGEYLHQIQ RIDGGQKTLL AEYRQDEQGR LVEANATHAY
HLYYEYNTAH RLTRWHDNDQ TWARYEYDAQ GRCVYTTCAD GFLTARFDYL PDRVVMTDGL
GQRSEFGFND LHLMSWEQSP LGHITRYEYD EVGNLLREIS PAGRVVEFTY LDDTGRVSTF
TDGSGHQWQY DYDDAQRLCG VTDPLGREWG WVYDAEGNPE RLTGPDASEV RFTWNRYGLL
TQVSDAAGEV QARLQYDHRQ RLLSATDAES RTRQLRYDRQ DRVVQWQRAD GARFRLGYRR
ASWTLPEQLI RPDDKEEQRQ YDRHNNLLSY VDGNGALWRQ TFGPFDLLTA RTDAEGRTWR
YEYDRESQQL IAVTAPDGSR WQWWLDADGR VIRERDMTGT ETHYGYDEDG LCIRVRNGEG
DTRHFLYDAR GLLLRETAPD DTLHYRYDAA GRLTEVSSAT AHVQLDYDLR DRVVREWHNG
TLLTRQYDDA ARTVTRTLTW DGDADDTTGT LAPLTSLFHY TRTGELRQVQ LPDGADLTLT
HDAAGRESLR TGGSGFVQQR EYDVMGWLTR EQSGAQHDGR LQPAQTREYR YDGAGNLTGV
RHNRDAEGYR LDATGRVQEM LSGGAGKPVD TTARFHYTRT GLPQEAGRLT EWQAGRLVQH
DDTHYQYDRA GRLIRKQVVQ PGYRPQVWQY RWDSRNQLRV VDTPNGERWL YRYDPFGRRV
GKRCDQKAEE TRYLWDGDQI AEIRHYRHGQ LIQRRHWVYN GWELVVQQRQ HTGGDWETDF
VTSSQNGTPQ ALFTPDGTLR WQVPKATLWG QRQTEKSESP DPGLAFAGQL RDSESGLCYN
RFRYYDPAGG CYVSPDPIGI AGGESNYGYV QNPNTRVDPL GLAGCAMGEI LADADKWSLA
KIGDRQKGMI KDKLSTVKER SKALNTKMRE HFNANEQKII SEWEKQTGMN WPTLSSGSRA
TPHHVIPIKN GGSNEWWNII PVQHPHTGTI HGTGSALRTH LPYQKDGGKL WNLLGY
