RHSC_DICD3
ID RHSC_DICD3 Reviewed; 1658 AA.
AC E0SGL7;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 1.
DT 03-AUG-2022, entry version 50.
DE RecName: Full=Putative deoxyribonuclease RhsC;
DE EC=3.1.-.-;
DE AltName: Full=DNase RhsC;
DE AltName: Full=Toxin RhsC;
GN Name=rhsC; OrderedLocusNames=Dda3937_04312;
OS Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Pectobacteriaceae; Dickeya.
OX NCBI_TaxID=198628;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3937;
RX PubMed=21217001; DOI=10.1128/jb.01513-10;
RA Glasner J.D., Yang C.H., Reverchon S., Hugouvieux-Cotte-Pattat N.,
RA Condemine G., Bohin J.P., Van Gijsegem F., Yang S., Franza T., Expert D.,
RA Plunkett G. III, San Francisco M.J., Charkowski A.O., Py B., Bell K.,
RA Rauscher L., Rodriguez-Palenzuela P., Toussaint A., Holeva M.C., He S.Y.,
RA Douet V., Boccara M., Blanco C., Toth I., Anderson B.D., Biehl B.S.,
RA Mau B., Flynn S.M., Barras F., Lindeberg M., Birch P.R., Tsuyumu S.,
RA Shi X., Hibbing M., Yap M.N., Carpentier M., Dassa E., Umehara M.,
RA Kim J.F., Rusch M., Soni P., Mayhew G.F., Fouts D.E., Gill S.R.,
RA Blattner F.R., Keen N.T., Perna N.T.;
RT "Genome sequence of the plant-pathogenic bacterium Dickeya dadantii 3937.";
RL J. Bacteriol. 193:2076-2077(2011).
RN [2]
RP FUNCTION AS A TOXIN, EXPRESSION IN E.COLI, AND DISRUPTION PHENOTYPE.
RC STRAIN=3937;
RX PubMed=23572593; DOI=10.1073/pnas.1300627110;
RA Koskiniemi S., Lamoureux J.G., Nikolakakis K.C., t'Kint de Roodenbeke C.,
RA Kaplan M.D., Low D.A., Hayes C.S.;
RT "Rhs proteins from diverse bacteria mediate intercellular competition.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:7032-7037(2013).
CC -!- FUNCTION: Putative toxic component of a toxin-immunity protein module,
CC which may function as a cellular contact-dependent growth inhibition
CC (CDI) system. Expression of the C-terminus (residues 1519-1658) in
CC E.coli inhibits growth but does not alter nucleoid structure. Its
CC effect is blocked by cognate immunity protein RhsIC.
CC {ECO:0000269|PubMed:23572593}.
CC -!- DISRUPTION PHENOTYPE: A double rhsC-rhsIC deletion is not outcompeted
CC by wild-type cells. {ECO:0000269|PubMed:23572593}.
CC -!- SIMILARITY: Belongs to the RHS/WapA nuclease family. {ECO:0000305}.
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DR EMBL; CP002038; ADM97666.1; -; Genomic_DNA.
DR AlphaFoldDB; E0SGL7; -.
DR SMR; E0SGL7; -.
DR STRING; 198628.Dda3937_04312; -.
DR EnsemblBacteria; ADM97666; ADM97666; Dda3937_04312.
DR KEGG; ddd:Dda3937_04312; -.
DR eggNOG; COG3209; Bacteria.
DR HOGENOM; CLU_001218_1_2_6; -.
DR OMA; WDMSLEL; -.
DR Proteomes; UP000006859; Chromosome.
DR GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR045351; DUF6531.
DR InterPro; IPR001826; RHS.
DR InterPro; IPR022385; Rhs_assc_core.
DR InterPro; IPR031325; RHS_repeat.
DR InterPro; IPR006530; YD.
DR Pfam; PF20148; DUF6531; 1.
DR Pfam; PF03527; RHS; 1.
DR Pfam; PF05593; RHS_repeat; 6.
DR TIGRFAMs; TIGR03696; Rhs_assc_core; 1.
DR TIGRFAMs; TIGR01643; YD_repeat_2x; 8.
PE 1: Evidence at protein level;
KW Hydrolase; Nuclease; Reference proteome; Repeat; Toxin; Virulence.
FT CHAIN 1..1658
FT /note="Putative deoxyribonuclease RhsC"
FT /id="PRO_0000423974"
FT REPEAT 821..857
FT /note="YD 1"
FT /evidence="ECO:0000255"
FT REPEAT 939..966
FT /note="YD 2"
FT /evidence="ECO:0000255"
FT REPEAT 1014..1050
FT /note="YD 3"
FT /evidence="ECO:0000255"
FT REPEAT 1326..1355
FT /note="YD 4"
FT /evidence="ECO:0000255"
FT REGION 393..494
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 419..434
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 436..452
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 466..484
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1658 AA; 184883 MW; 564F2FD489BA9DDF CRC64;
MSQDKKATLL SSEDAANQNF ATDNQISGGC AKCGCEVLIH YHYDSGKPVP NAPFILIDSN
KTEIHGKTDA NGLCKIYDMG CGTFELMLDE GSDDFKPRET VENNPVLQSN PAYATLAGEY
FTLFLLLRKQ GLVTYDADDS SDRHVDVDGA GIFTSIPKEY RKSYDRFWEL DKRINRGSRQ
LKQAINKIHH SLAAEVADKG GDDNAALMLF CEIALGFIPV VGQAMDVYSI GEWSWQSYQE
PARLEDPLHI AEGALCAIGV IPGLGDALKV SGRAIIRALK VGTPKELQFA IRTIRSLSDG
NLVKGLTKLR AELRNYGAQA KALLLKIHAA LKQVLAESKL KNNWIVSLMK DSFSAMITSL
EKLIAKYDSA LAYIESKFNE FIGKVITRVS GSARPKGSIA KAAEAPKAPA HAESQAAKPA
TPKSDNSITK ADDGKPTNAV SEKKRRTSSK ETEDISAGSN KGEQPDHAPQ QKKAGEEDNA
CKAGSDKCQG EGEPVDMATG YVVDWRTDVE LTGLLPLSMK RYYRSGGERK PGLLGALWRT
NWDMSLELEN GIATLTDGEL NQAVFVLPDE GAFSRAPSSP QWRLARQQGQ LVLQHVDGLR
YRFEHALGLQ LCLSAIEDRA GNRIALLWDR AELCWIVLPD GRLVHVETQQ RRIVKLTLCD
EHRQPLKTLS SYQYDAQGHL LSVRAGEGRN FDYQYSPQGW LLRWSDLAHT WVEHEYDAAG
RALRDRTAEG FWPGAFRYDD DAFTSHYHSG FGGVTTYVRD ARNNILLRRE PDGGEHRFEW
VDNQLAAEID PLGGRTEYQR NDWGQVTAVT LPDGSVHRYD YDDDGQLLAY TDPLGNAWHY
SRNAQGLVET ASDPEGRSWH HAYTTQGLLS AVNGPDGCEQ RYHYNRRGLL ERLEPGEAPA
VTFFYDGHDR LTARHIAHEQ GVQVRRWDYE GGRNTPSKVV YEDGSETRFG YDGEGNLTSV
TDALGQRYLF RYGAFDNLLE TTDPLGATVR YHYNAESEFA GVTNSQGQMW QYRFDTAGRL
SEERHYDGRV YRYDYDVAGQ LTRRTAPDGS RLEYGYDVGG RLSEIQACRA DGASEGATTF
TYDLSGRLLK AASPDAVVEY AYNRAGQVVS ERVNGEEVRT GYDDGGQRSV VEGLLSSLSL
GWQGGRLTTL SIGSHQPLTF SHTASGYEQR RSNGEGFALR HEWSATGLLS GQALDGVNGV
LERRYQYDVL DCLTGITDSH WGEQAFRLNG AGQVTAERRE QGRQRQARLF GYDSEQNLCE
VSAIAPDGAG RLSAKNAAVQ LSSGYDEAGR VTQRGGRQYQ YDACGRLVSR RESRPGFRPQ
ETRFEWDAQD RLVRVSLPDG ARWRYGYDAF GRRVSKVREG QVPSAQAVAR VAYRWDGDQL
SGQTQYRVDG SVTRAVQWVY EPGSFRPLAQ VEEQAGQTRL HYIVADLTGT ARELCSETGE
VHWRGEQGLW GPHREEKIPI PLRRYLGDAA NEEVYCELRY QGQVYDAETG LYYNRHRYYD
PELGQYISAD PIGLAGGLRP QGYVHNPMEW VDPFGLVGCP LKDSPLGKNG VELERTVSKK
GNVKVDTLFE NSNDAKNWAA EKLGPGKTRM YDSNGKWIGW QNKTGDSVYW GHNDWGKGVG
KSTYPHLNIN INGEKGHLFL RDKIINRGQW DDFSNAFK
