RHT1_WHEAT
ID RHT1_WHEAT Reviewed; 623 AA.
AC Q9ST59;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=DELLA protein RHT-1;
DE AltName: Full=Protein Rht-B1/Rht-D1;
DE AltName: Full=Reduced height protein 1;
GN Name=RHT1;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS GLN-61--GLU-64 DEL AND
RP GLN-62--GLU-64 DEL.
RC STRAIN=cv. Rht-B1b, and cv. Rht-D1b;
RX PubMed=10421366; DOI=10.1038/22307;
RA Peng J., Richards D.E., Hartley N.M., Murphy G.P., Devos K.M.,
RA Flintham J.E., Beales J., Fish L.J., Worland A.J., Pelica F., Sudhakar D.,
RA Christou P., Snape J.W., Gale M.D., Harberd N.P.;
RT "'Green revolution' genes encode mutant gibberellin response modulators.";
RL Nature 400:256-261(1999).
CC -!- FUNCTION: Probable transcriptional regulator that acts as a repressor
CC of the gibberellin (GA) signaling pathway. Probably acts by
CC participating in large multiprotein complexes that repress
CC transcription of GA-inducible genes. Upon GA application, it is
CC degraded by the proteasome, allowing the GA signaling pathway.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: The DELLA motif is required for its GA-induced degradation.
CC {ECO:0000305}.
CC -!- PTM: Phosphorylated. {ECO:0000250}.
CC -!- PTM: Ubiquitinated. Upon GA application it is ubiquitinated, leading to
CC its subsequent degradation (Probable). {ECO:0000305}.
CC -!- BIOTECHNOLOGY: This gene, also known as the 'green revolution gene' has
CC been introduced by conventional breeding procedures into several
CC cultivars. It produces shorter plants, increase grain yield at the
CC expense of straw biomass, and are more resistant to damage by wind and
CC rain. These wheats are short because they respond abnormally to GA.
CC -!- SIMILARITY: Belongs to the GRAS family. DELLA subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=All things dwarfed and
CC beautiful - Issue 70 of May 2006;
CC URL="https://web.expasy.org/spotlight/back_issues/070";
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ242531; CAB51555.1; -; Genomic_DNA.
DR AlphaFoldDB; Q9ST59; -.
DR SMR; Q9ST59; -.
DR STRING; 4565.Traes_4BS_2EE4988CD.1; -.
DR EnsemblPlants; TraesCAD_scaffold_027774_01G000100.1; TraesCAD_scaffold_027774_01G000100.1; TraesCAD_scaffold_027774_01G000100.
DR EnsemblPlants; TraesCS4D02G040400.1; TraesCS4D02G040400.1.cds1; TraesCS4D02G040400.
DR EnsemblPlants; TraesPAR_scaffold_021593_01G000100.1; TraesPAR_scaffold_021593_01G000100.1; TraesPAR_scaffold_021593_01G000100.
DR EnsemblPlants; TraesROB_scaffold_023654_01G000100.1; TraesROB_scaffold_023654_01G000100.1; TraesROB_scaffold_023654_01G000100.
DR EnsemblPlants; TraesWEE_scaffold_064278_01G000100.1; TraesWEE_scaffold_064278_01G000100.1; TraesWEE_scaffold_064278_01G000100.
DR Gramene; TraesCAD_scaffold_027774_01G000100.1; TraesCAD_scaffold_027774_01G000100.1; TraesCAD_scaffold_027774_01G000100.
DR Gramene; TraesCS4D02G040400.1; TraesCS4D02G040400.1.cds1; TraesCS4D02G040400.
DR Gramene; TraesPAR_scaffold_021593_01G000100.1; TraesPAR_scaffold_021593_01G000100.1; TraesPAR_scaffold_021593_01G000100.
DR Gramene; TraesROB_scaffold_023654_01G000100.1; TraesROB_scaffold_023654_01G000100.1; TraesROB_scaffold_023654_01G000100.
DR Gramene; TraesWEE_scaffold_064278_01G000100.1; TraesWEE_scaffold_064278_01G000100.1; TraesWEE_scaffold_064278_01G000100.
DR eggNOG; ENOG502QPMG; Eukaryota.
DR Proteomes; UP000019116; Unplaced.
DR ExpressionAtlas; Q9ST59; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.10.1290; -; 1.
DR InterPro; IPR038088; DELLA_N_sf.
DR InterPro; IPR030006; TF_DELLA.
DR InterPro; IPR021914; TF_DELLA_N.
DR InterPro; IPR005202; TF_GRAS.
DR PANTHER; PTHR31636; PTHR31636; 1.
DR PANTHER; PTHR31636:SF47; PTHR31636:SF47; 1.
DR Pfam; PF12041; DELLA; 1.
DR Pfam; PF03514; GRAS; 1.
DR PROSITE; PS50985; GRAS; 1.
PE 1: Evidence at protein level;
KW Gibberellin signaling pathway; Nucleus; Phosphoprotein; Reference proteome;
KW Repressor; Transcription; Transcription regulation; Ubl conjugation.
FT CHAIN 1..623
FT /note="DELLA protein RHT-1"
FT /id="PRO_0000132249"
FT DOMAIN 225..619
FT /note="GRAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 109..138
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 159..201
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 232..288
FT /note="Leucine repeat I (LRI)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 307..372
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 386..425
FT /note="Leucine repeat II (LRII)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 435..540
FT /note="PFYRE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 543..619
FT /note="SAW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 38..42
FT /note="DELLA motif"
FT MOTIF 239..243
FT /note="LxCxE motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 338..342
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT COMPBIAS 122..138
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 178..199
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 61..64
FT /note="Missing (in strain: cv. Rht-D1b; induces a dwarf
FT phenotype)"
FT VARIANT 62..64
FT /note="Missing (in strain: cv. Rht-B1b; induces a dwarf
FT phenotype)"
SQ SEQUENCE 623 AA; 65337 MW; F2BAC34996D0A84F CRC64;
MKREYQDAGG SGGGGGGMGS SEDKMMVSAA AGEGEEVDEL LAALGYKVRA SDMADVAQKL
EQLEMAMGMG GVGAGAAPDD SFATHLATDT VHYNPTDLSS WVESMLSELN APPPPLPPAP
QLNASTSSTV TGSGGYFDLP PSVDSSSSIY ALRPIPSPAG ATAPADLSAD SVRDPKRMRT
GGSSTSSSSS SSSSLGGGAR SSVVEAAPPV AAAANATPAL PVVVVDTQEA GIRLVHALLA
CAEAVQQENL SAAEALVKQI PLLAASQGGA MRKVAAYFGE ALARRVFRFR PQPDSSLLDA
AFADLLHAHF YESCPYLKFA HFTANQAILE AFAGCRRVHV VDFGIKQGMQ WPALLQALAL
RPGGPPSFRL TGVGPPQPDE TDALQQVGWK LAQFAHTIRV DFQYRGLVAA TLADLEPFML
QPEGEEDPNE EPEVIAVNSV FEMHRLLAQP GALEKVLGTV RAVRPRIVTV VEQEANHNSG
TFLDRFTESL HYYSTMFDSL EGGSSGGGPS EVSSGAAAAP AAAGTDQVMS EVYLGRQICN
VVACEGAERT ERHETLGQWR NRLGNAGFET VHLGSNAYKQ ASTLLALFAG GDGYKVEEKE
GCLTLGWHTR PLIATSAWRL AGP
