RHTA_ECOLI
ID RHTA_ECOLI Reviewed; 295 AA.
AC P0AA67; P36545; P75784;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Threonine/homoserine exporter RhtA;
GN Name=rhtA; Synonyms=ybiF; OrderedLocusNames=b0813, JW0798;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8021175; DOI=10.1128/jb.176.13.3928-3935.1994;
RA Lomovskaya O.L., Kidwell J.P., Matin A.;
RT "Characterization of the sigma 38-dependent expression of a core
RT Escherichia coli starvation gene, pexB.";
RL J. Bacteriol. 176:3928-3935(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=12648727; DOI=10.1016/s0923-2508(03)00036-6;
RA Livshits V.A., Zakataeva N.P., Aleshin V.V., Vitushkina M.V.;
RT "Identification and characterization of the new gene rhtA involved in
RT threonine and homoserine efflux in Escherichia coli.";
RL Res. Microbiol. 154:123-135(2003).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Involved in the efflux of threonine and homoserine. Can also
CC export other amino acids such as proline, serine, histidine and
CC cysteine. {ECO:0000269|PubMed:12648727}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:12648727}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:12648727}.
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. 10 TMS drug/metabolite exporter (DME) (TC 2.A.7.3) family.
CC {ECO:0000305}.
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DR EMBL; U04242; AAA21854.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73900.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35485.1; -; Genomic_DNA.
DR PIR; E64818; E64818.
DR RefSeq; NP_415334.1; NC_000913.3.
DR RefSeq; WP_001295297.1; NZ_STEB01000019.1.
DR AlphaFoldDB; P0AA67; -.
DR SMR; P0AA67; -.
DR BioGRID; 4259985; 17.
DR STRING; 511145.b0813; -.
DR TCDB; 2.A.7.3.6; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; P0AA67; -.
DR PRIDE; P0AA67; -.
DR EnsemblBacteria; AAC73900; AAC73900; b0813.
DR EnsemblBacteria; BAA35485; BAA35485; BAA35485.
DR GeneID; 947045; -.
DR KEGG; ecj:JW0798; -.
DR KEGG; eco:b0813; -.
DR PATRIC; fig|1411691.4.peg.1465; -.
DR EchoBASE; EB2055; -.
DR eggNOG; COG5006; Bacteria.
DR HOGENOM; CLU_057295_0_1_6; -.
DR InParanoid; P0AA67; -.
DR OMA; MWAAYIV; -.
DR PhylomeDB; P0AA67; -.
DR BioCyc; EcoCyc:EG12134-MON; -.
DR BioCyc; MetaCyc:EG12134-MON; -.
DR PRO; PR:P0AA67; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0042970; F:homoserine transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0015291; F:secondary active transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0015565; F:threonine efflux transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0042968; P:homoserine transport; IDA:EcoCyc.
DR GO; GO:0015826; P:threonine transport; IDA:EcoCyc.
DR InterPro; IPR000620; EamA_dom.
DR Pfam; PF00892; EamA; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..295
FT /note="Threonine/homoserine exporter RhtA"
FT /id="PRO_0000108162"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..38
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 60..71
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 93
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 115..118
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 119..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 140..146
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 168..175
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..200
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 222..237
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..258
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 259..262
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 263..283
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 284..295
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 30..135
FT /note="EamA 1"
FT DOMAIN 159..278
FT /note="EamA 2"
FT CONFLICT 268
FT /note="A -> R (in Ref. 1; AAA21854)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 295 AA; 31168 MW; EC830029E71BAD5F CRC64;
MPGSLRKMPV WLPIVILLVA MASIQGGASL AKSLFPLVGA PGVTALRLAL GTLILIAFFK
PWRLRFAKEQ RLPLLFYGVS LGGMNYLFYL SIQTVPLGIA VALEFTGPLA VALFSSRRPV
DFVWVVLAVL GLWFLLPLGQ DVSHVDLTGC ALALGAGACW AIYILSGQRA GAEHGPATVA
IGSLIAALIF VPIGALQAGE ALWHWSVIPL GLAVAILSTA LPYSLEMIAL TRLPTRTFGT
LMSMEPALAA VSGMIFLGET LTPIQLLALG AIIAASMGST LTVRKESKIK ELDIN
