RHTB_ECOLI
ID RHTB_ECOLI Reviewed; 206 AA.
AC P0AG34; P27847; Q2M8C9;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Homoserine/homoserine lactone efflux protein;
GN Name=rhtB; Synonyms=yigK; OrderedLocusNames=b3824, JW5585;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=1379743; DOI=10.1126/science.1379743;
RA Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT "Analysis of the Escherichia coli genome: DNA sequence of the region from
RT 84.5 to 86.5 minutes.";
RL Science 257:771-778(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP SEQUENCE REVISION.
RX PubMed=16397293; DOI=10.1093/nar/gkj405;
RA Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R.,
RA Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T.,
RA Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H.,
RA Thomson N.R., Wishart D., Wanner B.L.;
RT "Escherichia coli K-12: a cooperatively developed annotation snapshot
RT -- 2005.";
RL Nucleic Acids Res. 34:1-9(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION AS A TRANSPORTER.
RX PubMed=10386596; DOI=10.1016/s0014-5793(99)00625-0;
RA Zakataeva N.P., Aleshin V.V., Tokmakova I.L., Troshin P.V., Livshits V.A.;
RT "The novel transmembrane Escherichia coli proteins involved in the amino
RT acid efflux.";
RL FEBS Lett. 452:228-232(1999).
CC -!- FUNCTION: Conducts the efflux of homoserine and homoserine lactone.
CC {ECO:0000269|PubMed:10386596}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Rht family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA67620.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M87049; AAA67620.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U00096; AAT48223.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77477.1; -; Genomic_DNA.
DR RefSeq; WP_000171710.1; NZ_STEB01000021.1.
DR RefSeq; YP_026265.1; NC_000913.3.
DR AlphaFoldDB; P0AG34; -.
DR BioGRID; 4259612; 20.
DR STRING; 511145.b3824; -.
DR TCDB; 2.A.76.1.1; the resistance to homoserine/threonine (rhtb) family.
DR PaxDb; P0AG34; -.
DR PRIDE; P0AG34; -.
DR EnsemblBacteria; AAT48223; AAT48223; b3824.
DR EnsemblBacteria; BAE77477; BAE77477; BAE77477.
DR GeneID; 66672268; -.
DR GeneID; 948316; -.
DR KEGG; ecj:JW5585; -.
DR KEGG; eco:b3824; -.
DR PATRIC; fig|1411691.4.peg.2883; -.
DR EchoBASE; EB1437; -.
DR eggNOG; COG1280; Bacteria.
DR HOGENOM; CLU_079569_2_1_6; -.
DR InParanoid; P0AG34; -.
DR OMA; PQFINPH; -.
DR PhylomeDB; P0AG34; -.
DR BioCyc; EcoCyc:RHTB-MON; -.
DR BioCyc; MetaCyc:RHTB-MON; -.
DR PRO; PR:P0AG34; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0042970; F:homoserine transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0015565; F:threonine efflux transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0006865; P:amino acid transport; IBA:GO_Central.
DR GO; GO:0042968; P:homoserine transport; IDA:EcoCyc.
DR GO; GO:0015826; P:threonine transport; IDA:EcoCyc.
DR InterPro; IPR004778; Homoserine/Threonine_efflux.
DR InterPro; IPR001123; LeuE-type.
DR PANTHER; PTHR30086; PTHR30086; 1.
DR Pfam; PF01810; LysE; 1.
DR PIRSF; PIRSF006324; LeuE; 1.
DR TIGRFAMs; TIGR00949; 2A76; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..206
FT /note="Homoserine/homoserine lactone efflux protein"
FT /id="PRO_0000094730"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 206 AA; 22427 MW; 1164F17738509C8C CRC64;
MTLEWWFAYL LTSIILSLSP GSGAINTMTT SLNHGYRGAV ASIAGLQTGL AIHIVLVGVG
LGTLFSRSVI AFEVLKWAGA AYLIWLGIQQ WRAAGAIDLK SLASTQSRRH LFQRAVFVNL
TNPKSIVFLA ALFPQFIMPQ QPQLMQYIVL GVTTIVVDII VMIGYATLAQ RIALWIKGPK
QMKALNKIFG SLFMLVGALL ASARHA
