ID   RHTC_ECOLI              Reviewed;         206 AA.
AC   P0AG38; P27846; Q2M8C8;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Threonine efflux protein;
GN   Name=rhtC; Synonyms=yigJ; OrderedLocusNames=b3823, JW5586;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=1379743; DOI=10.1126/science.1379743;
RA   Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT   "Analysis of the Escherichia coli genome: DNA sequence of the region from
RT   84.5 to 86.5 minutes.";
RL   Science 257:771-778(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   SEQUENCE REVISION.
RX   PubMed=16397293; DOI=10.1093/nar/gkj405;
RA   Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R.,
RA   Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T.,
RA   Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H.,
RA   Thomson N.R., Wishart D., Wanner B.L.;
RT   "Escherichia coli K-12: a cooperatively developed annotation snapshot
RT   -- 2005.";
RL   Nucleic Acids Res. 34:1-9(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-107.
RC   STRAIN=K12;
RX   PubMed=3027506; DOI=10.1007/bf00430442;
RA   Irino N., Nakayama K., Nakayama H.;
RT   "The recQ gene of Escherichia coli K12: primary structure and evidence for
RT   SOS regulation.";
RL   Mol. Gen. Genet. 205:298-304(1986).
RN   [6]
RP   FUNCTION AS A TRANSPORTER.
RX   PubMed=10386596; DOI=10.1016/s0014-5793(99)00625-0;
RA   Zakataeva N.P., Aleshin V.V., Tokmakova I.L., Troshin P.V., Livshits V.A.;
RT   "The novel transmembrane Escherichia coli proteins involved in the amino
RT   acid efflux.";
RL   FEBS Lett. 452:228-232(1999).
RN   [7]
RP   TOPOLOGY [LARGE SCALE ANALYSIS], AND SUBCELLULAR LOCATION.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- FUNCTION: Conducts the efflux of threonine.
CC       {ECO:0000269|PubMed:10386596}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:15919996}.
CC   -!- SIMILARITY: Belongs to the Rht family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA67619.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=M30198; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; M87049; AAA67619.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; U00096; AAT48222.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77478.1; -; Genomic_DNA.
DR   EMBL; M30198; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; WP_000928824.1; NZ_SSZK01000046.1.
DR   RefSeq; YP_026264.1; NC_000913.3.
DR   AlphaFoldDB; P0AG38; -.
DR   BioGRID; 4263363; 8.
DR   STRING; 511145.b3823; -.
DR   TCDB; 2.A.76.1.2; the resistance to homoserine/threonine (rhtb) family.
DR   PaxDb; P0AG38; -.
DR   PRIDE; P0AG38; -.
DR   EnsemblBacteria; AAT48222; AAT48222; b3823.
DR   EnsemblBacteria; BAE77478; BAE77478; BAE77478.
DR   GeneID; 66672269; -.
DR   GeneID; 948317; -.
DR   KEGG; ecj:JW5586; -.
DR   KEGG; eco:b3823; -.
DR   PATRIC; fig|1411691.4.peg.2884; -.
DR   EchoBASE; EB1436; -.
DR   eggNOG; COG1280; Bacteria.
DR   HOGENOM; CLU_079569_0_1_6; -.
DR   InParanoid; P0AG38; -.
DR   OMA; MAWLHNI; -.
DR   PhylomeDB; P0AG38; -.
DR   BioCyc; EcoCyc:RHTC-MON; -.
DR   BioCyc; MetaCyc:RHTC-MON; -.
DR   PRO; PR:P0AG38; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR   GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015565; F:threonine efflux transmembrane transporter activity; IDA:EcoCyc.
DR   GO; GO:0006865; P:amino acid transport; IBA:GO_Central.
DR   GO; GO:0015826; P:threonine transport; IDA:EcoCyc.
DR   InterPro; IPR004778; Homoserine/Threonine_efflux.
DR   InterPro; IPR001123; LeuE-type.
DR   PANTHER; PTHR30086; PTHR30086; 1.
DR   Pfam; PF01810; LysE; 1.
DR   PIRSF; PIRSF006324; LeuE; 1.
DR   TIGRFAMs; TIGR00949; 2A76; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..206
FT                   /note="Threonine efflux protein"
FT                   /id="PRO_0000094736"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        22..43
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        44..64
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        65..66
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        67..87
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        88..149
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        150..173
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        174..206
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   206 AA;  22474 MW;  F64017878CC6D50D CRC64;
     MLMLFLTVAM VHIVALMSPG PDFFFVSQTA VSRSRKEAMM GVLGITCGVM VWAGIALLGL
     HLIIEKMAWL HTLIMVGGGL YLCWMGYQML RGALKKEAVS APAPQVELAK SGRSFLKGLL
     TNLANPKAII YFGSVFSLFV GDNVGTTARW GIFALIIVET LAWFTVVASL FALPQMRRGY
     QRLAKWIDGF AGALFAGFGI HLIISR