ID   RIA1_USTMD              Reviewed;         381 AA.
AC   A0A0U2WFX7;
DT   18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT   16-MAR-2016, sequence version 1.
DT   25-MAY-2022, entry version 15.
DE   RecName: Full=Regulator of itaconic acid biosynthesis {ECO:0000303|PubMed:26639528};
DE   AltName: Full=Itaconic acid/2-hydroxyparaconate biosynthesis cluster protein RIA1 {ECO:0000305};
GN   Name=RIA1 {ECO:0000303|PubMed:26639528}; ORFNames=UMAG_05080;
OS   Ustilago maydis (Corn smut fungus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC   Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX   NCBI_TaxID=5270;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=MB215;
RX   PubMed=26639528; DOI=10.1111/1751-7915.12329;
RA   Geiser E., Przybilla S.K., Friedrich A., Buckel W., Wierckx N., Blank L.M.,
RA   Boelker M.;
RT   "Ustilago maydis produces itaconic acid via the unusual intermediate trans-
RT   aconitate.";
RL   Microb. Biotechnol. 9:116-126(2016).
RN   [2]
RP   FUNCTION.
RX   PubMed=27750034; DOI=10.1016/j.ymben.2016.10.006;
RA   Geiser E., Przybilla S.K., Engel M., Kleineberg W., Buettner L.,
RA   Sarikaya E., Hartog T.D., Klankermayer J., Leitner W., Boelker M.,
RA   Blank L.M., Wierckx N.;
RT   "Genetic and biochemical insights into the itaconate pathway of Ustilago
RT   maydis enable enhanced production.";
RL   Metab. Eng. 38:427-435(2016).
CC   -!- FUNCTION: Transcription factor that specifically regulates the
CC       expression of the gene cluster that mediates the biosynthesis of
CC       itaconic acid and 2-hydroxyparaconate (PubMed:26639528,).
CC       {ECO:0000269|PubMed:26639528}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC   -!- DISRUPTION PHENOTYPE: Abolishes the production of itaconic acid
CC       (PubMed:26639528). {ECO:0000269|PubMed:26639528}.
CC   -!- CAUTION: Contains a degenerate basic motif not likely to bind DNA.
CC       {ECO:0000255|PROSITE-ProRule:PRU00981}.
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DR   EMBL; KT852988; ALS30795.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0U2WFX7; -.
DR   VEuPathDB; FungiDB:UMAG_05080; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   3: Inferred from homology;
KW   Nucleus; Transcription; Transcription regulation.
FT   CHAIN           1..381
FT                   /note="Regulator of itaconic acid biosynthesis"
FT                   /id="PRO_0000438680"
FT   DOMAIN          90..142
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          81..103
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          90..103
FT                   /note="Basic motif; degenerate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          104..142
FT                   /note="Helix-loop-helix motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          212..337
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          353..381
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        212..226
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        227..261
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        286..300
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        306..337
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   381 AA;  41513 MW;  0B7320E319747E9A CRC64;
     MRFAGMSCDD ERPANMFDLM APQLASTSCN EHYFSTADLG ASTLYATTTD APATIAGILT
     PQPATLAPMY STCPVRFDDQ RSAPASTSVS GKRKHSDVEK DRRRSISNGF AVLQNVLHNE
     SNAKPISKSI LLQQACDEIR ELRKKLDAST TIISRFGLEN LFVPTPSSTH ASPPNASSRI
     YSPINQASDV LADTRRASIS TSATPILYSE EKRKANAKRR HSYDGSWQAS DRGSIDDEAS
     ASASASASAS SSASCSSSSH THSDDTDCDD TDTDIPAESA LKERTKRHKA RSKKERDRTK
     PRYRPKPSTN RSPTPSSCAS STPNSPPTSS NRNRDLQQAI LSLLLELPSH LEDVKNKRRA
     SQPTELADPS SVKSRSKKRH R