ID   RIB73_ECOLX             Reviewed;         316 AA.
AC   P0DV87;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Retron Ec73 putative ribosyltransferase/DNA-binding protein {ECO:0000303|Ref.2};
DE   AltName: Full=Orf316 {ECO:0000303|PubMed:1712012};
GN   ORFNames=Ga0175965_1549, RG59_11965 {ECO:0000303|Ref.2};
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1] {ECO:0000312|EMBL:M64113}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC   STRAIN=C1-23;
RX   PubMed=1712012; DOI=10.1128/jb.173.13.4171-4181.1991;
RA   Sun J., Inouye M., Inouye S.;
RT   "Association of a retroelement with a P4-like cryptic prophage (retronphage
RT   phi R73) integrated into the selenocystyl tRNA gene of Escherichia coli.";
RL   J. Bacteriol. 173:4171-4181(1991).
RN   [2] {ECO:0000312|EMBL:APL18793.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=M10;
RA   Jiang J., Xu Z., Zhou Y., Zhao H., Leung F.C.C.;
RT   "Vertical and Horizontal Evolutionary Story implied from E. coli complete
RT   genomes.";
RL   Submitted (NOV-2014) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, AND PROPHAGE EXCISION AND REINFECTION.
RC   STRAIN=C1-23;
RX   PubMed=1709758; DOI=10.1126/science.1709758;
RA   Inouye S., Sunshine M.G., Six E.W., Inouye M.;
RT   "Retronphage phi R73: an E. coli phage that contains a retroelement and
RT   integrates into a tRNA gene.";
RL   Science 252:969-971(1991).
RN   [4]
RP   FUNCTION IN ANTIVIRAL DEFENSE, IDENTIFICATION AS A RETRON, AND MUTAGENESIS
RP   OF GLU-117.
RC   STRAIN=M10;
RX   PubMed=33157039; DOI=10.1016/j.cell.2020.09.065;
RA   Millman A., Bernheim A., Stokar-Avihail A., Fedorenko T., Voichek M.,
RA   Leavitt A., Oppenheimer-Shaanan Y., Sorek R.;
RT   "Bacterial Retrons Function In Anti-Phage Defense.";
RL   Cell 183:1551-1561(2020).
CC   -!- FUNCTION: Possible ribosyltransferase/DNA-binding component of
CC       antiviral defense system retron Ec73, composed of a non-coding RNA
CC       (ncRNA) followed by this protein then a reverse transcriptase (RT).
CC       Expression of this retron confers protection against bacteriophages
CC       SECphi4, SECphi6, SECphi27 and P1. At multiplicity of infection (MOI)
CC       of 0.02 cultures grow normally when infected with SECphi4 without
CC       collapsing, at MOI 2 cultures enter growth stasis.
CC       {ECO:0000269|PubMed:33157039}.
CC   -!- DISRUPTION PHENOTYPE: Not required for msDNA production.
CC       {ECO:0000269|PubMed:1712012}.
CC   -!- MISCELLANEOUS: Part of pro-retronphage phiR73, which is homologous to
CC       bacteriophage P4 (PubMed:1712012). With the help of P2 bacteriophage,
CC       is excised and packaged into an infectious virion. Retronphage phiR73
CC       can lysogenize a new host strain, reintegrating its genome into the
CC       selC gene of the host chromosome and enabling the newly formed lysogens
CC       to produce msDNA-Ec73 (PubMed:1709758). {ECO:0000269|PubMed:1709758,
CC       ECO:0000269|PubMed:1712012}.
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DR   EMBL; M64113; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP010200; APL18793.1; -; Genomic_DNA.
PE   1: Evidence at protein level;
KW   Antiviral defense.
FT   CHAIN           1..316
FT                   /note="Retron Ec73 putative ribosyltransferase/DNA-binding
FT                   protein"
FT                   /id="PRO_0000456032"
FT   MUTAGEN         117
FT                   /note="E->Q: No longer protects against bacteriophage
FT                   SECphi4, SECphi6, SECphi27 or P1 infection."
FT                   /evidence="ECO:0000269|PubMed:33157039"
SQ   SEQUENCE   316 AA;  36478 MW;  1B1DA76F970148A1 CRC64;
     MLTQLKKNGT EVSRATALFS SFVEKNKVKC PGNVKKFVFL CGANKNNGEP SARRLELINF
     SERYLNNCHF FLAELVFKEL STDEESLSDN LLDIEADLSK LADHIIIVLE SYSSFTELGA
     FAYSKQLRKK LIIVNNTKFI NEKSFINMGP IKAITQQSQQ SGHFLHYKMT EGIESIERSD
     GIGEIFDPLY DILSKNDRAI SRTLKKEELD PSSNFNKDSV RFIHDVIFVC GPLQLNELIE
     IITKIFGTES HYKKNLLKHL GILIAIRIIS CTNGIYYSLY KEYYFKYDFD IDNISSMFKV
     FFLKNKPERM RVYENI