ID   RIB86_ECOLX             Reviewed;         307 AA.
AC   P0DV88;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Retron Ec86 putative ribosyltransferase/DNA-binding protein {ECO:0000303|PubMed:2466573};
DE   AltName: Full=ORF223 {ECO:0000303|PubMed:1722556};
GN   ORFNames=LM2_00875;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1] {ECO:0000312|EMBL:X60206}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=B / AC2514;
RX   PubMed=1722556; DOI=10.1111/j.1365-2958.1991.tb00810.x;
RA   Lim D.;
RT   "Structure of two retrons of Escherichia coli and their common chromosomal
RT   insertion site.";
RL   Mol. Microbiol. 5:1863-1872(1991).
RN   [2] {ECO:0000312|EMBL:M24408}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 181-307.
RC   STRAIN=B / AC2514;
RX   PubMed=2466573; DOI=10.1016/0092-8674(89)90693-4;
RA   Lim D., Maas W.K.;
RT   "Reverse transcriptase-dependent synthesis of a covalently linked, branched
RT   DNA-RNA compound in E. coli B.";
RL   Cell 56:891-904(1989).
RN   [3]
RP   FUNCTION IN ANTIVIRAL DEFENSE, AND IDENTIFICATION AS A RETRON.
RC   STRAIN=BL21 (DE3);
RX   PubMed=33157039; DOI=10.1016/j.cell.2020.09.065;
RA   Millman A., Bernheim A., Stokar-Avihail A., Fedorenko T., Voichek M.,
RA   Leavitt A., Oppenheimer-Shaanan Y., Sorek R.;
RT   "Bacterial Retrons Function In Anti-Phage Defense.";
RL   Cell 183:1551-1561(2020).
CC   -!- FUNCTION: Possible ribosyltransferase/DNA-binding component of
CC       antiviral defense system retron Ec86, composed of a non-coding RNA
CC       (ncRNA), a ribosyltransferase/DNA-binding protein and a reverse
CC       transcriptase (RT). Expression of the 3-gene retron confers protection
CC       against bacteriophages T5. At multiplicity of infection (MOI) of 0.02
CC       cultures grow normally when infected with T5 without collapsing, at MOI
CC       2 cultures enter growth stasis. {ECO:0000269|PubMed:33157039}.
CC   -!- CAUTION: DNA from strain B / AC2514 has genes in the order
CC       ribosyltransferase-ncRNA-RT (PubMed:2466573, PubMed:1722556). The data
CC       presented in Millman et al says genes are encoded in the order ncRNA-
CC       ribosyltransferase-RT. {ECO:0000269|PubMed:1722556,
CC       ECO:0000269|PubMed:2466573, ECO:0000305|PubMed:33157039}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=M24408; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=X60206; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; M24408; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; X60206; -; NOT_ANNOTATED_CDS; Genomic_DNA.
PE   1: Evidence at protein level;
KW   Antiviral defense.
FT   CHAIN           1..307
FT                   /note="Retron Ec86 putative ribosyltransferase/DNA-binding
FT                   protein"
FT                   /id="PRO_0000456033"
SQ   SEQUENCE   307 AA;  35483 MW;  13C336A40C4841A1 CRC64;
     MNKKFTDEQQ QQLIGHLTKK GFYRGANIKI TIFLCGGDVA NHQSWRHQLS QFLAKFSDVD
     IFYPEDLFDD LLAGQGQHSL LSLENILAEA VDVIILFPES PGSFTELGAF SNNENLRRKL
     ICIQDAKFKS KRSFINYGPV RLLRKFNSKS VLRCSSNELK EMCDSSIDVA RKLRLYKKLM
     ASIKKVRKEN KVSKDIGNIL YAERFLLPCI YLLDSVNYRT LCELAFKAIK QDDVLSKIIV
     RSVVSRLINE RKILQMTDGY QVTALGASYV RSVFDRKTLD RLRLEIMNFE NRRKSTFNYD
     KIPYAHP