RIBM_STRDJ
ID RIBM_STRDJ Reviewed; 213 AA.
AC K4REQ6; Q2VCE9;
DT 28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2013, sequence version 1.
DT 25-MAY-2022, entry version 41.
DE RecName: Full=Riboflavin/roseoflavin transporter RibM {ECO:0000305};
GN Name=ribM {ECO:0000303|PubMed:17541777};
GN ORFNames=BN159_7145 {ECO:0000312|EMBL:CCK31524.1};
OS Streptomyces davaonensis (strain DSM 101723 / JCM 4913 / KCC S-0913 / 768).
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces.
OX NCBI_TaxID=1214101;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=DSM 101723 / JCM 4913 / KCC S-0913 / 768;
RX PubMed=17541777; DOI=10.1007/s00203-007-0258-1;
RA Grill S., Yamaguchi H., Wagner H., Zwahlen L., Kusch U., Mack M.;
RT "Identification and characterization of two Streptomyces davawensis
RT riboflavin biosynthesis gene clusters.";
RL Arch. Microbiol. 188:377-387(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 101723 / JCM 4913 / KCC S-0913 / 768;
RX PubMed=23043000; DOI=10.1128/jb.01592-12;
RA Jankowitsch F., Schwarz J., Ruckert C., Gust B., Szczepanowski R., Blom J.,
RA Pelzer S., Kalinowski J., Mack M.;
RT "Genome sequence of the bacterium Streptomyces davawensis JCM 4913 and
RT heterologous production of the unique antibiotic roseoflavin.";
RL J. Bacteriol. 194:6818-6827(2012).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND BIOTECHNOLOGY.
RX PubMed=22136195; DOI=10.1186/1472-6750-11-119;
RA Hemberger S., Pedrolli D.B., Stolz J., Vogl C., Lehmann M., Mack M.;
RT "RibM from Streptomyces davawensis is a riboflavin/roseoflavin transporter
RT and may be useful for the optimization of riboflavin production strains.";
RL BMC Biotechnol. 11:119-119(2011).
RN [4]
RP FUNCTION.
RX PubMed=28406895; DOI=10.1371/journal.pntd.0005513;
RA Balcazar D.E., Vanrell M.C., Romano P.S., Pereira C.A., Goldbaum F.A.,
RA Bonomi H.R., Carrillo C.;
RT "The superfamily keeps growing: Identification in trypanosomatids of RibJ,
RT the first riboflavin transporter family in protists.";
RL PLoS Negl. Trop. Dis. 11:E0005513-E0005513(2017).
CC -!- FUNCTION: Transports riboflavin and roseoflavin (PubMed:17541777,
CC PubMed:22136195, PubMed:28406895). Can also transport FMN and FAD
CC (PubMed:28406895). May confer roseoflavin resistance to S.davawensis,
CC which naturally produces this antibiotic during stationary growth phase
CC (PubMed:22136195). {ECO:0000269|PubMed:17541777,
CC ECO:0000269|PubMed:22136195, ECO:0000269|PubMed:28406895}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:22136195};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- BIOTECHNOLOGY: Introduction of ribM into a high performance B.subtilis
CC riboflavin production strain may improve riboflavin production process.
CC {ECO:0000269|PubMed:22136195}.
CC -!- SIMILARITY: Belongs to the nicotinamide ribonucleoside (NR) uptake
CC permease (TC 4.B.1) family. {ECO:0000305}.
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DR EMBL; DQ248961; ABB90841.1; -; Genomic_DNA.
DR EMBL; HE971709; CCK31524.1; -; Genomic_DNA.
DR RefSeq; WP_015661853.1; NC_020504.1.
DR AlphaFoldDB; K4REQ6; -.
DR SMR; K4REQ6; -.
DR STRING; 1214101.BN159_7145; -.
DR EnsemblBacteria; CCK31524; CCK31524; BN159_7145.
DR KEGG; sdv:BN159_7145; -.
DR PATRIC; fig|1214101.3.peg.7237; -.
DR eggNOG; COG3201; Bacteria.
DR HOGENOM; CLU_076589_0_0_11; -.
DR OMA; WPDAYIF; -.
DR OrthoDB; 1301128at2; -.
DR Proteomes; UP000008043; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0034257; F:nicotinamide riboside transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR006419; NMN_transpt_PnuC.
DR PANTHER; PTHR36122; PTHR36122; 1.
DR Pfam; PF04973; NMN_transporter; 1.
DR TIGRFAMs; TIGR01528; NMN_trans_PnuC; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..213
FT /note="Riboflavin/roseoflavin transporter RibM"
FT /id="PRO_0000443722"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..193
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 213 AA; 23749 MW; E83762FD7C4F0F12 CRC64;
MNWLNSEAFV LFDQHIIWSD MVGNILGLIT LALGFRRSLW TWPVQFLSGL VLFGAFYGHL
TGSAGKQAVV MAVALYGWYQ WNRGTDKAAD GKVSVRFATW AERGAMIAAA AVGTVAVALL
FKAYPSLSWD PWPDAYIFVG TIVAMYAQAR GMVEFWFAWL LVDLVGVPLN FANGYAFSGF
VYVIYGALVL WGMRDWWLRS RRDSRPVLEG APA