ID   RIBN_RHIL3              Reviewed;         302 AA.
AC   Q1MIM3;
DT   11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Riboflavin transporter {ECO:0000303|PubMed:23935051};
GN   Name=ribN {ECO:0000303|PubMed:23935051}; OrderedLocusNames=RL1692;
OS   Rhizobium leguminosarum bv. viciae (strain 3841).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=216596;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=3841;
RX   PubMed=16640791; DOI=10.1186/gb-2006-7-4-r34;
RA   Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F.,
RA   Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H.,
RA   East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I.,
RA   Chillingworth T., Clarke K., Cronin A., Davis P., Fraser A., Hance Z.,
RA   Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H.,
RA   Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J.;
RT   "The genome of Rhizobium leguminosarum has recognizable core and accessory
RT   components.";
RL   Genome Biol. 7:R34.1-R34.20(2006).
RN   [2]
RP   FUNCTION AS A TRANSPORTER, SUBCELLULAR LOCATION, INDUCTION, AND GENE NAME.
RC   STRAIN=3841;
RX   PubMed=23935051; DOI=10.1128/jb.00644-13;
RA   Garcia Angulo V.A., Bonomi H.R., Posadas D.M., Serer M.I., Torres A.G.,
RA   Zorreguieta A., Goldbaum F.A.;
RT   "Identification and characterization of RibN, a novel family of riboflavin
RT   transporters from Rhizobium leguminosarum and other Proteobacteria.";
RL   J. Bacteriol. 195:4611-4619(2013).
CC   -!- FUNCTION: Transports riboflavin into the cell. Can also transport FMN
CC       and FAD. Required for normal nodule development during colonization of
CC       pea plant roots. {ECO:0000269|PubMed:23935051}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23935051};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:23935051}.
CC   -!- INDUCTION: Negatively regulated by riboflavin, probably via an FMN
CC       riboswitch. {ECO:0000269|PubMed:23935051}.
CC   -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC       superfamily. 10 TMS drug/metabolite exporter (DME) (TC 2.A.7.3) family.
CC       {ECO:0000305}.
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DR   EMBL; AM236080; CAK07187.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q1MIM3; -.
DR   SMR; Q1MIM3; -.
DR   STRING; 216596.RL1692; -.
DR   TCDB; 2.A.7.3.54; the drug/metabolite transporter (dmt) superfamily.
DR   EnsemblBacteria; CAK07187; CAK07187; RL1692.
DR   KEGG; rle:RL1692; -.
DR   eggNOG; COG0697; Bacteria.
DR   HOGENOM; CLU_032828_0_0_5; -.
DR   OMA; TMKLAFP; -.
DR   Proteomes; UP000006575; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR000620; EamA_dom.
DR   Pfam; PF00892; EamA; 2.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Repeat; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..302
FT                   /note="Riboflavin transporter"
FT                   /id="PRO_0000424626"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        44..64
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        109..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        158..178
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        191..213
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        227..247
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        264..284
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          30..151
FT                   /note="EamA 1"
FT   DOMAIN          170..291
FT                   /note="EamA 2"
SQ   SEQUENCE   302 AA;  32511 MW;  BB527A8D2776865B CRC64;
     MKDMNQTSLA VEPSRAVVGA LWMVLAGIAF SLLNVVTQWL TMKLAFPSAS AAFWQYGFAF
     LFSLPFLKRL GLAAMRTHYP WRHLTRVVLA ALGVEAWVAG LAAVPIWQAI ALVMTSPFFI
     ILGARLFLGE RVGPARWAAT AAGFTGAMII LQPWSDGIGW AALLPVLSAL LWGASSLITK
     SLTGIERPET ITVWLLVLLT PINGGLALAA GFAVPTGATL ALFLLAGLLT AVAQYFLTLA
     YAAADAAYVQ PFDDLKLPLN VLAGWLFFGY APAGYLWLGA ALILSASLFI MRNEMRRERK
     PA