ID   RIBR_BACSU              Reviewed;         230 AA.
AC   P94465;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=RNA-binding riboflavin kinase RibR;
DE            EC=2.7.1.26;
GN   Name=ribR; Synonyms=ytnK; OrderedLocusNames=BSU29300;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SB25;
RA   Gusarov I.;
RL   Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9387221; DOI=10.1099/00221287-143-11-3431;
RA   Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT   "Sequencing and functional annotation of the Bacillus subtilis genes in the
RT   200 kb rrnB-dnaB region.";
RL   Microbiology 143:3431-3441(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=15668000; DOI=10.1016/j.femsle.2004.11.038;
RA   Solovieva I.M., Kreneva R.A., Errais Lopes L., Perumov D.A.;
RT   "The riboflavin kinase encoding gene ribR of Bacillus subtilis is a part of
RT   a 10 kb operon, which is negatively regulated by the yrzC gene product.";
RL   FEMS Microbiol. Lett. 243:51-58(2005).
RN   [5]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=17590224; DOI=10.1111/j.1574-6968.2007.00817.x;
RA   Higashitsuji Y., Angerer A., Berghaus S., Hobl B., Mack M.;
RT   "RibR, a possible regulator of the Bacillus subtilis riboflavin
RT   biosynthetic operon, in vivo interacts with the 5'-untranslated leader of
RT   rib mRNA.";
RL   FEMS Microbiol. Lett. 274:48-54(2007).
CC   -!- FUNCTION: May be directly involved in the regulation of the rib genes.
CC       C-terminal part of RibR specifically binds to RFN of the rib leader of
CC       the riboflavin biosynthetic operon. The RFN element is a sequence
CC       within the rib-leader mRNA reported to serve as a receptor for an FMN-
CC       dependent riboswitch. Possibly, RibR produces the comodulator FMN
CC       through its own N-terminal flavokinase activity. FMN-activated RibR may
CC       stabilize the anti-anti terminator structure of RFN mRNA, causing
CC       transcription termination of the rib genes in trans.
CC       {ECO:0000269|PubMed:15668000, ECO:0000269|PubMed:17590224}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + riboflavin = ADP + FMN + H(+); Xref=Rhea:RHEA:14357,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:57986,
CC         ChEBI:CHEBI:58210, ChEBI:CHEBI:456216; EC=2.7.1.26;
CC   -!- INDUCTION: Repressed by the HTH-type transcriptional regulator CymR.
CC       {ECO:0000269|PubMed:17590224}.
CC   -!- DISRUPTION PHENOTYPE: Mutants lacking this gene are deregulated with
CC       respect to flavin (riboflavin, FMN and FAD) biosynthesis.
CC       {ECO:0000269|PubMed:15668000}.
CC   -!- MISCELLANEOUS: Overexpression of RibR suppresses the riboflavin-
CC       overproducing ribC-defective phenotype, which is the major riboflavin
CC       kinase responsible of FMN biosynthesis by riboflavin phosphorylation
CC       (PubMed:17590224 and PubMed:15668000).
CC   -!- SIMILARITY: Belongs to the RibR family. {ECO:0000305}.
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DR   EMBL; Y09721; CAA70887.1; -; Genomic_DNA.
DR   EMBL; AF008220; AAC00333.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14890.1; -; Genomic_DNA.
DR   PIR; G69692; G69692.
DR   RefSeq; NP_390808.1; NC_000964.3.
DR   RefSeq; WP_003229393.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; P94465; -.
DR   SMR; P94465; -.
DR   STRING; 224308.BSU29300; -.
DR   PaxDb; P94465; -.
DR   PRIDE; P94465; -.
DR   EnsemblBacteria; CAB14890; CAB14890; BSU_29300.
DR   GeneID; 937367; -.
DR   KEGG; bsu:BSU29300; -.
DR   PATRIC; fig|224308.179.peg.3184; -.
DR   eggNOG; COG0196; Bacteria.
DR   InParanoid; P94465; -.
DR   OMA; NIDTQHE; -.
DR   PhylomeDB; P94465; -.
DR   BioCyc; BSUB:BSU29300-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008531; F:riboflavin kinase activity; IBA:GO_Central.
DR   GO; GO:0009398; P:FMN biosynthetic process; IBA:GO_Central.
DR   GO; GO:0009231; P:riboflavin biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0006771; P:riboflavin metabolic process; IBA:GO_Central.
DR   Gene3D; 2.40.30.30; -; 1.
DR   InterPro; IPR023468; Riboflavin_kinase.
DR   InterPro; IPR015865; Riboflavin_kinase_bac/euk.
DR   InterPro; IPR023465; Riboflavin_kinase_dom_sf.
DR   PANTHER; PTHR22749; PTHR22749; 1.
DR   Pfam; PF01687; Flavokinase; 1.
DR   SMART; SM00904; Flavokinase; 1.
DR   SUPFAM; SSF82114; SSF82114; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Flavoprotein; FMN; Nucleotide-binding; Reference proteome;
KW   Riboflavin biosynthesis; Transferase.
FT   CHAIN           1..230
FT                   /note="RNA-binding riboflavin kinase RibR"
FT                   /id="PRO_0000194147"
SQ   SEQUENCE   230 AA;  26229 MW;  593108B81768EA17 CRC64;
     MTIIAGTVVK GKQLGRKLGF PTANVDAKIH GLRNGVYGVL ATVNHQFHLG VMNIGVKPTV
     GSNLEKTLEI FLFDFHRDIY GEKIECSILF KIREERRFDS LESLTKQIKK DISCVAKRFE
     LIGIMAPNKK ESLLSHQELN LPDLCFYKKC NNLYGVNRGV YNVIDNWFFE YGITQVAYRR
     IYILSFLSFL KEDNPKVSSK YIRFGAGGLA DKLNRFISSY VEESEENILG